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Differential expression of aldolase, alpha tubulin and thioredoxin peroxidase in peripheral blood mononuclear cells from dengue fever and dengue hemorrhagic fever patients.

Abstract

We determined the differential expression levels of proteins in peripheral blood mononuclear cells of patients with dengue fever (DF) and dengue hemorrhagic fever (DHF). Proteins were subjected to two-dimensional electrophoresis, mass spectrometry and Western blot analysis. We identified 8 proteins that were 2-fold or more up-regulated in patients compared to healthy control, three of which, aldolase, thioredoxin peroxidase and alpha tubulin, were related to dengue infection. Both thioredoxin peroxidase and alpha tubulin were over-expressed 4.9 and 3.3 times respectively in DHF compared to DF patients while aldolase was up-regulated 2.2 times in DF compared to DHF patients. Alpha tubulin and thioredoxin peroxidase have the potential to be utilized as biomarkers for DHF.

Authors+Show Affiliations

Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19323035

Citation

Thayan, Ravindran, et al. "Differential Expression of Aldolase, Alpha Tubulin and Thioredoxin Peroxidase in Peripheral Blood Mononuclear Cells From Dengue Fever and Dengue Hemorrhagic Fever Patients." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 40, no. 1, 2009, pp. 56-65.
Thayan R, Huat TL, See LL, et al. Differential expression of aldolase, alpha tubulin and thioredoxin peroxidase in peripheral blood mononuclear cells from dengue fever and dengue hemorrhagic fever patients. Southeast Asian J Trop Med Public Health. 2009;40(1):56-65.
Thayan, R., Huat, T. L., See, L. L., Khairullah, N. S., Yusof, R., & Devi, S. (2009). Differential expression of aldolase, alpha tubulin and thioredoxin peroxidase in peripheral blood mononuclear cells from dengue fever and dengue hemorrhagic fever patients. The Southeast Asian Journal of Tropical Medicine and Public Health, 40(1), 56-65.
Thayan R, et al. Differential Expression of Aldolase, Alpha Tubulin and Thioredoxin Peroxidase in Peripheral Blood Mononuclear Cells From Dengue Fever and Dengue Hemorrhagic Fever Patients. Southeast Asian J Trop Med Public Health. 2009;40(1):56-65. PubMed PMID: 19323035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of aldolase, alpha tubulin and thioredoxin peroxidase in peripheral blood mononuclear cells from dengue fever and dengue hemorrhagic fever patients. AU - Thayan,Ravindran, AU - Huat,Tan Lian, AU - See,Lucy Lum Chai, AU - Khairullah,Nor Shahidah, AU - Yusof,Rohana, AU - Devi,Shamala, PY - 2009/3/28/entrez PY - 2009/3/28/pubmed PY - 2009/6/27/medline SP - 56 EP - 65 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 40 IS - 1 N2 - We determined the differential expression levels of proteins in peripheral blood mononuclear cells of patients with dengue fever (DF) and dengue hemorrhagic fever (DHF). Proteins were subjected to two-dimensional electrophoresis, mass spectrometry and Western blot analysis. We identified 8 proteins that were 2-fold or more up-regulated in patients compared to healthy control, three of which, aldolase, thioredoxin peroxidase and alpha tubulin, were related to dengue infection. Both thioredoxin peroxidase and alpha tubulin were over-expressed 4.9 and 3.3 times respectively in DHF compared to DF patients while aldolase was up-regulated 2.2 times in DF compared to DHF patients. Alpha tubulin and thioredoxin peroxidase have the potential to be utilized as biomarkers for DHF. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/19323035/Differential_expression_of_aldolase_alpha_tubulin_and_thioredoxin_peroxidase_in_peripheral_blood_mononuclear_cells_from_dengue_fever_and_dengue_hemorrhagic_fever_patients_ L2 - http://www.diseaseinfosearch.org/result/2187 DB - PRIME DP - Unbound Medicine ER -