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Three-minute-long chemiluminescent immunoassay using dually accelerated immunoreaction by infrared heating and passive mixing.
Anal Chem. 2009 May 15; 81(10):4043-7.AC

Abstract

A rapid chemiluminescent (CL) immunoassay method was developed by integrating a newly designed infrared-radiation technique with a pressure-driven fluidic system. The fluidic system combined a three-dimensional helical glass tube for rapid mixing of immunoreagents with two spiral glass tubes for magnetic separation and CL detection, respectively. The mixture passively formed in the helical glass tube could be quickly heated and kept at about 37 degrees C by the infrared radiation. The immunoreaction could be finished within 90 s due to the dual acceleration by the improved mass transport and enhanced reaction kinetics. The horseradish peroxidase-labeled sandwich immunocomplex formed on paramagnetic particles was then separated by a permanent magnet and mixed with CL substrate in a long spiral tube, and the detection mixture was immediately injected through another spiral tube in front of the photomultiplier for collecting the CL signal. Using alpha-fetoprotein as a proof-of-principle analyte, the immunoassay could be completed within 3 min with a linear calibration range of 0.2-90 microg/L. This programmable method showed acceptable detection and fabrication reproducibility and good accuracy, indicating a promise of automated high-throughput clinical application.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education of China, Department of Chemistry, Nanjing University, Nanjing 210093, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19323531

Citation

Liu, Hong, et al. "Three-minute-long Chemiluminescent Immunoassay Using Dually Accelerated Immunoreaction By Infrared Heating and Passive Mixing." Analytical Chemistry, vol. 81, no. 10, 2009, pp. 4043-7.
Liu H, Yang Z, Yan F, et al. Three-minute-long chemiluminescent immunoassay using dually accelerated immunoreaction by infrared heating and passive mixing. Anal Chem. 2009;81(10):4043-7.
Liu, H., Yang, Z., Yan, F., Xu, Y., & Ju, H. (2009). Three-minute-long chemiluminescent immunoassay using dually accelerated immunoreaction by infrared heating and passive mixing. Analytical Chemistry, 81(10), 4043-7. https://doi.org/10.1021/ac900245x
Liu H, et al. Three-minute-long Chemiluminescent Immunoassay Using Dually Accelerated Immunoreaction By Infrared Heating and Passive Mixing. Anal Chem. 2009 May 15;81(10):4043-7. PubMed PMID: 19323531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three-minute-long chemiluminescent immunoassay using dually accelerated immunoreaction by infrared heating and passive mixing. AU - Liu,Hong, AU - Yang,Zhanjun, AU - Yan,Feng, AU - Xu,Yueming, AU - Ju,Huangxian, PY - 2009/3/28/entrez PY - 2009/3/28/pubmed PY - 2009/7/10/medline SP - 4043 EP - 7 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 10 N2 - A rapid chemiluminescent (CL) immunoassay method was developed by integrating a newly designed infrared-radiation technique with a pressure-driven fluidic system. The fluidic system combined a three-dimensional helical glass tube for rapid mixing of immunoreagents with two spiral glass tubes for magnetic separation and CL detection, respectively. The mixture passively formed in the helical glass tube could be quickly heated and kept at about 37 degrees C by the infrared radiation. The immunoreaction could be finished within 90 s due to the dual acceleration by the improved mass transport and enhanced reaction kinetics. The horseradish peroxidase-labeled sandwich immunocomplex formed on paramagnetic particles was then separated by a permanent magnet and mixed with CL substrate in a long spiral tube, and the detection mixture was immediately injected through another spiral tube in front of the photomultiplier for collecting the CL signal. Using alpha-fetoprotein as a proof-of-principle analyte, the immunoassay could be completed within 3 min with a linear calibration range of 0.2-90 microg/L. This programmable method showed acceptable detection and fabrication reproducibility and good accuracy, indicating a promise of automated high-throughput clinical application. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19323531/Three_minute_long_chemiluminescent_immunoassay_using_dually_accelerated_immunoreaction_by_infrared_heating_and_passive_mixing_ L2 - https://doi.org/10.1021/ac900245x DB - PRIME DP - Unbound Medicine ER -