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Immunohistochemical analyses of beta-catenin and cyclin D1 expression in giant cell tumor of bone (GCTB): a possible role of Wnt pathway in GCTB tumorigenesis.
Pathol Res Pract. 2009; 205(9):626-33.PR

Abstract

Giant cell tumor of bone (GCTB) is a benign neoplasm but occasionally shows local recurrence, and histologically consists of osteoclast-like giant cells (GC) and stromal mononuclear cells (SC), which are capable of proliferation and osteoblastic differentiation. Activation of Wnt signaling can induce osteoblast differentiation and osteoclastgenesis during bone resorption process. This study analyzed the profiles of beta-catenin and cyclin D1 expression in GCTB to elucidate an involvement of Wnt pathway in tumorigenesis. We performed immunohistochemistry for beta-catenin, cyclin D1, and Ki-67 in 16 GCTB tumors, including 5 recurrent cases that were surgically resected. All 16 cases of GCTB displayed beta-catenin, cyclin D1, and Ki-67 expression. Immunoreactivity for beta-catenin was observed in nuclei of SC and GC. Cyclin D1 immunoreactivity was found mainly in nuclei of GC, while Ki-67 immunoreactivity was restricted to nuclei of SC. The nuclear beta-catenin labeling index (LI) in both SC (60.6 vs. 41.8%, p=0.074) and GC (41.7 vs. 20.1%, p=0.095) was higher in recurrent tumors than in primary tumors in all the 4 cases. However, Ki-67 LI in SC (18.8 vs. 19.9%, p=0.851) and cyclin D1 LI in GC (55.4 vs. 70.1%, p=0.225) were not higher in recurrent tumors than in primary tumors. Our results suggested activation of Wnt/ beta-catenin pathway in GCTB tumorigenesis. Since cyclin D1 in GC was never associated with the expression of the well-known proliferative marker Ki-67, cyclin D1 expression might play a role in GC formation instead of promoting cell proliferation during GCTB tumorigenesis. Importantly, it was suggested that the nuclear beta-catenin staining level might be associated with tumor recurrence in GCTB.

Authors+Show Affiliations

Department of Orthopedic Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19324500

Citation

Matsubayashi, Shohei, et al. "Immunohistochemical Analyses of Beta-catenin and Cyclin D1 Expression in Giant Cell Tumor of Bone (GCTB): a Possible Role of Wnt Pathway in GCTB Tumorigenesis." Pathology, Research and Practice, vol. 205, no. 9, 2009, pp. 626-33.
Matsubayashi S, Nakashima M, Kumagai K, et al. Immunohistochemical analyses of beta-catenin and cyclin D1 expression in giant cell tumor of bone (GCTB): a possible role of Wnt pathway in GCTB tumorigenesis. Pathol Res Pract. 2009;205(9):626-33.
Matsubayashi, S., Nakashima, M., Kumagai, K., Egashira, M., Naruke, Y., Kondo, H., Hayashi, T., & Shindo, H. (2009). Immunohistochemical analyses of beta-catenin and cyclin D1 expression in giant cell tumor of bone (GCTB): a possible role of Wnt pathway in GCTB tumorigenesis. Pathology, Research and Practice, 205(9), 626-33. https://doi.org/10.1016/j.prp.2009.02.011
Matsubayashi S, et al. Immunohistochemical Analyses of Beta-catenin and Cyclin D1 Expression in Giant Cell Tumor of Bone (GCTB): a Possible Role of Wnt Pathway in GCTB Tumorigenesis. Pathol Res Pract. 2009;205(9):626-33. PubMed PMID: 19324500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical analyses of beta-catenin and cyclin D1 expression in giant cell tumor of bone (GCTB): a possible role of Wnt pathway in GCTB tumorigenesis. AU - Matsubayashi,Shohei, AU - Nakashima,Masahiro, AU - Kumagai,Kenji, AU - Egashira,Masayuki, AU - Naruke,Yuki, AU - Kondo,Hisayoshi, AU - Hayashi,Tomayoshi, AU - Shindo,Hiroyuki, Y1 - 2009/03/25/ PY - 2008/07/18/received PY - 2009/02/15/revised PY - 2009/02/25/accepted PY - 2009/3/28/entrez PY - 2009/3/28/pubmed PY - 2009/10/29/medline SP - 626 EP - 33 JF - Pathology, research and practice JO - Pathol. Res. Pract. VL - 205 IS - 9 N2 - Giant cell tumor of bone (GCTB) is a benign neoplasm but occasionally shows local recurrence, and histologically consists of osteoclast-like giant cells (GC) and stromal mononuclear cells (SC), which are capable of proliferation and osteoblastic differentiation. Activation of Wnt signaling can induce osteoblast differentiation and osteoclastgenesis during bone resorption process. This study analyzed the profiles of beta-catenin and cyclin D1 expression in GCTB to elucidate an involvement of Wnt pathway in tumorigenesis. We performed immunohistochemistry for beta-catenin, cyclin D1, and Ki-67 in 16 GCTB tumors, including 5 recurrent cases that were surgically resected. All 16 cases of GCTB displayed beta-catenin, cyclin D1, and Ki-67 expression. Immunoreactivity for beta-catenin was observed in nuclei of SC and GC. Cyclin D1 immunoreactivity was found mainly in nuclei of GC, while Ki-67 immunoreactivity was restricted to nuclei of SC. The nuclear beta-catenin labeling index (LI) in both SC (60.6 vs. 41.8%, p=0.074) and GC (41.7 vs. 20.1%, p=0.095) was higher in recurrent tumors than in primary tumors in all the 4 cases. However, Ki-67 LI in SC (18.8 vs. 19.9%, p=0.851) and cyclin D1 LI in GC (55.4 vs. 70.1%, p=0.225) were not higher in recurrent tumors than in primary tumors. Our results suggested activation of Wnt/ beta-catenin pathway in GCTB tumorigenesis. Since cyclin D1 in GC was never associated with the expression of the well-known proliferative marker Ki-67, cyclin D1 expression might play a role in GC formation instead of promoting cell proliferation during GCTB tumorigenesis. Importantly, it was suggested that the nuclear beta-catenin staining level might be associated with tumor recurrence in GCTB. SN - 1618-0631 UR - https://www.unboundmedicine.com/medline/citation/19324500/Immunohistochemical_analyses_of_beta_catenin_and_cyclin_D1_expression_in_giant_cell_tumor_of_bone__GCTB_:_a_possible_role_of_Wnt_pathway_in_GCTB_tumorigenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0344-0338(09)00072-7 DB - PRIME DP - Unbound Medicine ER -