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The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings.
Chemosphere. 2009 Jun; 75(11):1468-76.C

Abstract

Zinc (Zn) is a necessary element for plants, but excess Zn can be detrimental. To investigate Zn toxicity, rapeseed (Brassica napus) seedlings were treated with 0.07-1.12 mM Zn for 7d. Inhibition of plant growth along with root damage, chlorosis and decreased chlorophyll (a and b) content in newly expanded leaves (the second and third leaves formed following cotyledons) were found under Zn stress. The Zn content increased in plants under external Zn stress, while concentrations of phosphorus, copper, iron, manganese and magnesium reduced significantly, especially in roots. Meanwhile, increased lipid peroxidation was detected biochemically and histochemically. Compared with controls, NADH oxidase and peroxidase (POD) activity increased in leaves and roots of plants under high Zn, but superoxide dismutase (SOD), catalase and ascorbate peroxidase activities decreased. The changes in glutathione S-transferase activity and in ascorbic acid, dehydroascorbate, non-protein thiols and glutathione contents were also measured under Zn stress. Isoforms of SOD and POD were separated using non-denaturing polyacrylamide gel electrophoresis and their activities were analyzed. Our results suggested that excess Zn exerts its toxicity partially through disturbing nutrient balance and inducing oxidative stress in plants. These data will be helpful for better understanding of toxicity of Zn and the adaptive mechanism in Zn non-hyperaccumulator plants.

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Authors+Show Affiliations

Wang C
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environmental Science and Engineering, Hohai University, Nanjing 210098, China.
Zhang SH
No affiliation info available
Wang PF
No affiliation info available
Hou J
No affiliation info available
Zhang WJ
No affiliation info available
Li W
No affiliation info available
Lin ZP
No affiliation info available

MeSH

AntioxidantsBrassica rapaChlorophyllGlutathione TransferaseLipid PeroxidationMineralsOxidative StressOxidoreductasesPeroxidasesPlant LeavesPlant ProteinsPlant RootsSeedlingsZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19328518

Citation

Wang, Chao, et al. "The Effect of Excess Zn On Mineral Nutrition and Antioxidative Response in Rapeseed Seedlings." Chemosphere, vol. 75, no. 11, 2009, pp. 1468-76.
Wang C, Zhang SH, Wang PF, et al. The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings. Chemosphere. 2009;75(11):1468-76.
Wang, C., Zhang, S. H., Wang, P. F., Hou, J., Zhang, W. J., Li, W., & Lin, Z. P. (2009). The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings. Chemosphere, 75(11), 1468-76. https://doi.org/10.1016/j.chemosphere.2009.02.033
Wang C, et al. The Effect of Excess Zn On Mineral Nutrition and Antioxidative Response in Rapeseed Seedlings. Chemosphere. 2009;75(11):1468-76. PubMed PMID: 19328518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings. AU - Wang,Chao, AU - Zhang,Song He, AU - Wang,Pei Fang, AU - Hou,Jun, AU - Zhang,Wen Jing, AU - Li,Wei, AU - Lin,Zhi Ping, Y1 - 2009/03/27/ PY - 2008/09/13/received PY - 2009/02/09/revised PY - 2009/02/11/accepted PY - 2009/3/31/entrez PY - 2009/3/31/pubmed PY - 2009/6/18/medline SP - 1468 EP - 76 JF - Chemosphere JO - Chemosphere VL - 75 IS - 11 N2 - Zinc (Zn) is a necessary element for plants, but excess Zn can be detrimental. To investigate Zn toxicity, rapeseed (Brassica napus) seedlings were treated with 0.07-1.12 mM Zn for 7d. Inhibition of plant growth along with root damage, chlorosis and decreased chlorophyll (a and b) content in newly expanded leaves (the second and third leaves formed following cotyledons) were found under Zn stress. The Zn content increased in plants under external Zn stress, while concentrations of phosphorus, copper, iron, manganese and magnesium reduced significantly, especially in roots. Meanwhile, increased lipid peroxidation was detected biochemically and histochemically. Compared with controls, NADH oxidase and peroxidase (POD) activity increased in leaves and roots of plants under high Zn, but superoxide dismutase (SOD), catalase and ascorbate peroxidase activities decreased. The changes in glutathione S-transferase activity and in ascorbic acid, dehydroascorbate, non-protein thiols and glutathione contents were also measured under Zn stress. Isoforms of SOD and POD were separated using non-denaturing polyacrylamide gel electrophoresis and their activities were analyzed. Our results suggested that excess Zn exerts its toxicity partially through disturbing nutrient balance and inducing oxidative stress in plants. These data will be helpful for better understanding of toxicity of Zn and the adaptive mechanism in Zn non-hyperaccumulator plants. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/19328518/The_effect_of_excess_Zn_on_mineral_nutrition_and_antioxidative_response_in_rapeseed_seedlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(09)00220-3 DB - PRIME DP - Unbound Medicine ER -