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Influence of salinity on the localization and expression of the CFTR chloride channel in the ionocytes of juvenile Dicentrarchus labrax exposed to seawater and freshwater.

Abstract

The European sea-bass, Dicentrarchus labrax is a euryhaline teleost whose high osmoregulatory abilities allow sea-lagoon migrations. In order to investigate the mechanism underlying the acclimation of juvenile fish to salinity, CFTR was studied in long-term (6 months) freshwater (FW)- and seawater (SW)-exposed fish, and in short-term (from day 0 to day 30) FW-exposed fish. Cellular and molecular approaches were combined to determine the functions of CFTR in the gills, posterior intestine and kidney. In the kidney, the expression of CFTR transcripts and protein is low. After a direct transfer from SW to FW, the CFTR mRNA is down-regulated in the gills within 1 day, followed by a protein decrease over 7 days. In the posterior intestine, first there is a protein decrease within one day and secondly at the mRNA level in 14 days. While in the gills the regulation is transcriptional, in the posterior intestine, there is first a post-transcriptional regulation followed by a transcriptional regulation after 14 days in FW. Over a long-term exposure, there is a transcriptional regulation in both organs. Coupled to other ion transports, CFTR contributes to ion regulation and thus to the adaptation of the European sea-bass to sea-lagoon transitions.

Authors+Show Affiliations

Université Montpellier 2, UMR5119-UM2-CNRS-IFREMER Ecolag, AEO team, cc092, Montpellier Cedex 05, 34095, France. Charlotte.Bodinier@univ-montp2.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19328865

Citation

Bodinier, Charlotte, et al. "Influence of Salinity On the Localization and Expression of the CFTR Chloride Channel in the Ionocytes of Juvenile Dicentrarchus Labrax Exposed to Seawater and Freshwater." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 153, no. 3, 2009, pp. 345-51.
Bodinier C, Lorin-Nebel C, Charmantier G, et al. Influence of salinity on the localization and expression of the CFTR chloride channel in the ionocytes of juvenile Dicentrarchus labrax exposed to seawater and freshwater. Comp Biochem Physiol, Part A Mol Integr Physiol. 2009;153(3):345-51.
Bodinier, C., Lorin-Nebel, C., Charmantier, G., & Boulo, V. (2009). Influence of salinity on the localization and expression of the CFTR chloride channel in the ionocytes of juvenile Dicentrarchus labrax exposed to seawater and freshwater. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 153(3), pp. 345-51. doi:10.1016/j.cbpa.2009.03.011.
Bodinier C, et al. Influence of Salinity On the Localization and Expression of the CFTR Chloride Channel in the Ionocytes of Juvenile Dicentrarchus Labrax Exposed to Seawater and Freshwater. Comp Biochem Physiol, Part A Mol Integr Physiol. 2009;153(3):345-51. PubMed PMID: 19328865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of salinity on the localization and expression of the CFTR chloride channel in the ionocytes of juvenile Dicentrarchus labrax exposed to seawater and freshwater. AU - Bodinier,Charlotte, AU - Lorin-Nebel,Catherine, AU - Charmantier,Guy, AU - Boulo,Viviane, Y1 - 2009/03/27/ PY - 2008/10/31/received PY - 2009/03/17/revised PY - 2009/03/18/accepted PY - 2009/3/31/entrez PY - 2009/3/31/pubmed PY - 2009/8/27/medline SP - 345 EP - 51 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. VL - 153 IS - 3 N2 - The European sea-bass, Dicentrarchus labrax is a euryhaline teleost whose high osmoregulatory abilities allow sea-lagoon migrations. In order to investigate the mechanism underlying the acclimation of juvenile fish to salinity, CFTR was studied in long-term (6 months) freshwater (FW)- and seawater (SW)-exposed fish, and in short-term (from day 0 to day 30) FW-exposed fish. Cellular and molecular approaches were combined to determine the functions of CFTR in the gills, posterior intestine and kidney. In the kidney, the expression of CFTR transcripts and protein is low. After a direct transfer from SW to FW, the CFTR mRNA is down-regulated in the gills within 1 day, followed by a protein decrease over 7 days. In the posterior intestine, first there is a protein decrease within one day and secondly at the mRNA level in 14 days. While in the gills the regulation is transcriptional, in the posterior intestine, there is first a post-transcriptional regulation followed by a transcriptional regulation after 14 days in FW. Over a long-term exposure, there is a transcriptional regulation in both organs. Coupled to other ion transports, CFTR contributes to ion regulation and thus to the adaptation of the European sea-bass to sea-lagoon transitions. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/19328865/Influence_of_salinity_on_the_localization_and_expression_of_the_CFTR_chloride_channel_in_the_ionocytes_of_juvenile_Dicentrarchus_labrax_exposed_to_seawater_and_freshwater_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(09)00084-1 DB - PRIME DP - Unbound Medicine ER -