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Hypothermic Liver Machine Perfusion With EKPS-1 Solution vs Aqix RS-I Solution: In Vivo Feasibility Study in a Pig Transplantation Model.
Transplant Proc. 2009 Mar; 41(2):617-21.TP

Abstract

OBJECTIVE

Hypothermic machine perfusion (HMP) is superior to simple cold storage for kidney preservation. We previous observed in a porcine liver transplantation model increased tumor necrosis factor-alpha (TNF-alpha) production eventually leading to poor recipient survival after HMP using standard kidney perfusion solution (KPS-1) compared with simple cold storage. We compared two solutions for HMP preservation of the liver: enriched KPS-1 (EKPS-1) and Aqix RS-I.

METHODS

Pig livers were obtained after cold flushing with histidine-tryptophan-ketoglutarate solution. Subsequently, the livers were subjected to dual-vessel perfusion with two preservation solutions: EKPS-1 (n = 6) and Aqix RS-I (n = 3). After HMP preservation and transplantation, graft and recipient survival, hepatocellular damage (aspartate aminotransferase concentration), TNF-alpha production, and endothelial cell damage (hyaluronic acid clearance) were recorded.

RESULTS

No primary graft nonfunction was observed. Recipient survival at postoperative day 3 was similar in both groups (33%). Aspartate aminotransferase concentration measured in serum samples after reperfusion was similar in both groups. After reperfusion, TNF-alpha concentration was higher and hyaluronic acid clearance was lower in the EKPS-1 group vs the Aqix RS-I group at 60, 120, and 180 minutes (all P < .05).

CONCLUSION

Hypothermic machine perfusion provided adequate longer term graft survival. After reperfusion, TNF-alpha production seems to be reduced, and endothelial cell dysfunction remains pronounced with Aqix RS-1 solution compared with EKPS-1 solution.

Authors+Show Affiliations

Abdominal Transplant Surgery Section, Department of Surgery, Catholic University of Leuven, Leuven, Belgium. Katrien.vekemans@med.kuleuven.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19328939

Citation

Vekemans, K, et al. "Hypothermic Liver Machine Perfusion With EKPS-1 Solution Vs Aqix RS-I Solution: in Vivo Feasibility Study in a Pig Transplantation Model." Transplantation Proceedings, vol. 41, no. 2, 2009, pp. 617-21.
Vekemans K, Liu Q, Heedfeld V, et al. Hypothermic Liver Machine Perfusion With EKPS-1 Solution vs Aqix RS-I Solution: In Vivo Feasibility Study in a Pig Transplantation Model. Transplant Proc. 2009;41(2):617-21.
Vekemans, K., Liu, Q., Heedfeld, V., Van de Vel, K., Wylin, T., Pirenne, J., & Monbaliu, D. (2009). Hypothermic Liver Machine Perfusion With EKPS-1 Solution vs Aqix RS-I Solution: In Vivo Feasibility Study in a Pig Transplantation Model. Transplantation Proceedings, 41(2), 617-21. https://doi.org/10.1016/j.transproceed.2008.12.022
Vekemans K, et al. Hypothermic Liver Machine Perfusion With EKPS-1 Solution Vs Aqix RS-I Solution: in Vivo Feasibility Study in a Pig Transplantation Model. Transplant Proc. 2009;41(2):617-21. PubMed PMID: 19328939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypothermic Liver Machine Perfusion With EKPS-1 Solution vs Aqix RS-I Solution: In Vivo Feasibility Study in a Pig Transplantation Model. AU - Vekemans,K, AU - Liu,Q, AU - Heedfeld,V, AU - Van de Vel,K, AU - Wylin,T, AU - Pirenne,J, AU - Monbaliu,D, PY - 2009/3/31/entrez PY - 2009/3/31/pubmed PY - 2009/7/30/medline SP - 617 EP - 21 JF - Transplantation proceedings JO - Transplant Proc VL - 41 IS - 2 N2 - OBJECTIVE: Hypothermic machine perfusion (HMP) is superior to simple cold storage for kidney preservation. We previous observed in a porcine liver transplantation model increased tumor necrosis factor-alpha (TNF-alpha) production eventually leading to poor recipient survival after HMP using standard kidney perfusion solution (KPS-1) compared with simple cold storage. We compared two solutions for HMP preservation of the liver: enriched KPS-1 (EKPS-1) and Aqix RS-I. METHODS: Pig livers were obtained after cold flushing with histidine-tryptophan-ketoglutarate solution. Subsequently, the livers were subjected to dual-vessel perfusion with two preservation solutions: EKPS-1 (n = 6) and Aqix RS-I (n = 3). After HMP preservation and transplantation, graft and recipient survival, hepatocellular damage (aspartate aminotransferase concentration), TNF-alpha production, and endothelial cell damage (hyaluronic acid clearance) were recorded. RESULTS: No primary graft nonfunction was observed. Recipient survival at postoperative day 3 was similar in both groups (33%). Aspartate aminotransferase concentration measured in serum samples after reperfusion was similar in both groups. After reperfusion, TNF-alpha concentration was higher and hyaluronic acid clearance was lower in the EKPS-1 group vs the Aqix RS-I group at 60, 120, and 180 minutes (all P < .05). CONCLUSION: Hypothermic machine perfusion provided adequate longer term graft survival. After reperfusion, TNF-alpha production seems to be reduced, and endothelial cell dysfunction remains pronounced with Aqix RS-1 solution compared with EKPS-1 solution. SN - 0041-1345 UR - https://www.unboundmedicine.com/medline/citation/19328939/Hypothermic_Liver_Machine_Perfusion_With_EKPS_1_Solution_vs_Aqix_RS_I_Solution:_In_Vivo_Feasibility_Study_in_a_Pig_Transplantation_Model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(08)01665-5 DB - PRIME DP - Unbound Medicine ER -