Tags

Type your tag names separated by a space and hit enter

13C-breath tests for sucrose digestion in congenital sucrase isomaltase-deficient and sacrosidase-supplemented patients.
J Pediatr Gastroenterol Nutr. 2009 Apr; 48(4):412-8.JP

Abstract

BACKGROUND

Congenital sucrase-isomaltase deficiency (CSID) is characterized by absence or deficiency of the mucosal sucrase-isomaltase enzyme. Specific diagnosis requires upper gastrointestinal biopsy with evidence of low to absent sucrase enzyme activity and normal histology. The hydrogen breath test (BT) is useful, but is not specific for confirmation of CSID. We investigated a more specific 13C-sucrose labeled BT.

OBJECTIVES

Determine whether CSID can be detected with the 13C-sucrose BT without duodenal biopsy sucrase assay, and if the 13C-sucrose BT can document restoration of sucrose digestion by CSID patients after oral supplementation with sacrosidase (Sucraid).

METHODS

Ten CSID patients were diagnosed by low biopsy sucrase activity. Ten controls were children who underwent endoscopy and biopsy because of dyspepsia or chronic diarrhea with normal mucosal enzymes activity and histology. Uniformly labeled 13C-glucose and 13C-sucrose loads were orally administered. 13CO2 breath enrichments were assayed using an infrared spectrophotometer. In CSID patients, the 13C-sucrose load was repeated adding Sucraid. Sucrose digestion and oxidation were calculated as a mean percent coefficient of glucose oxidation averaged between 30 and 90 minutes.

RESULTS

Classification of patients by 13C-sucrose BT percent coefficient of glucose oxidation agreed with biopsy sucrase activity. The breath test also documented the return to normal of sucrose digestion and oxidation after supplementation of CSID patients with Sucraid.

CONCLUSIONS

13C-sucrose BT is an accurate and specific noninvasive confirmatory test for CSID and for enzyme replacement management.

Authors+Show Affiliations

US Department of Agriculture Agricultural Research Service, Children's Nutrition Research Center, Departments of Pediatrics-Nutrition, Baylor College of Medicine, Houston, TX 77030, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19330928

Citation

Robayo-Torres, Claudia C., et al. "13C-breath Tests for Sucrose Digestion in Congenital Sucrase Isomaltase-deficient and Sacrosidase-supplemented Patients." Journal of Pediatric Gastroenterology and Nutrition, vol. 48, no. 4, 2009, pp. 412-8.
Robayo-Torres CC, Opekun AR, Quezada-Calvillo R, et al. 13C-breath tests for sucrose digestion in congenital sucrase isomaltase-deficient and sacrosidase-supplemented patients. J Pediatr Gastroenterol Nutr. 2009;48(4):412-8.
Robayo-Torres, C. C., Opekun, A. R., Quezada-Calvillo, R., Villa, X., Smith, E. O., Navarrete, M., Baker, S. S., & Nichols, B. L. (2009). 13C-breath tests for sucrose digestion in congenital sucrase isomaltase-deficient and sacrosidase-supplemented patients. Journal of Pediatric Gastroenterology and Nutrition, 48(4), 412-8.
Robayo-Torres CC, et al. 13C-breath Tests for Sucrose Digestion in Congenital Sucrase Isomaltase-deficient and Sacrosidase-supplemented Patients. J Pediatr Gastroenterol Nutr. 2009;48(4):412-8. PubMed PMID: 19330928.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 13C-breath tests for sucrose digestion in congenital sucrase isomaltase-deficient and sacrosidase-supplemented patients. AU - Robayo-Torres,Claudia C, AU - Opekun,Antone R, AU - Quezada-Calvillo,Roberto, AU - Villa,Xavier, AU - Smith,E O, AU - Navarrete,Marilyn, AU - Baker,Susan S, AU - Nichols,Buford L, PY - 2009/3/31/entrez PY - 2009/3/31/pubmed PY - 2009/7/29/medline SP - 412 EP - 8 JF - Journal of pediatric gastroenterology and nutrition JO - J. Pediatr. Gastroenterol. Nutr. VL - 48 IS - 4 N2 - BACKGROUND: Congenital sucrase-isomaltase deficiency (CSID) is characterized by absence or deficiency of the mucosal sucrase-isomaltase enzyme. Specific diagnosis requires upper gastrointestinal biopsy with evidence of low to absent sucrase enzyme activity and normal histology. The hydrogen breath test (BT) is useful, but is not specific for confirmation of CSID. We investigated a more specific 13C-sucrose labeled BT. OBJECTIVES: Determine whether CSID can be detected with the 13C-sucrose BT without duodenal biopsy sucrase assay, and if the 13C-sucrose BT can document restoration of sucrose digestion by CSID patients after oral supplementation with sacrosidase (Sucraid). METHODS: Ten CSID patients were diagnosed by low biopsy sucrase activity. Ten controls were children who underwent endoscopy and biopsy because of dyspepsia or chronic diarrhea with normal mucosal enzymes activity and histology. Uniformly labeled 13C-glucose and 13C-sucrose loads were orally administered. 13CO2 breath enrichments were assayed using an infrared spectrophotometer. In CSID patients, the 13C-sucrose load was repeated adding Sucraid. Sucrose digestion and oxidation were calculated as a mean percent coefficient of glucose oxidation averaged between 30 and 90 minutes. RESULTS: Classification of patients by 13C-sucrose BT percent coefficient of glucose oxidation agreed with biopsy sucrase activity. The breath test also documented the return to normal of sucrose digestion and oxidation after supplementation of CSID patients with Sucraid. CONCLUSIONS: 13C-sucrose BT is an accurate and specific noninvasive confirmatory test for CSID and for enzyme replacement management. SN - 1536-4801 UR - https://www.unboundmedicine.com/medline/citation/19330928/13C_breath_tests_for_sucrose_digestion_in_congenital_sucrase_isomaltase_deficient_and_sacrosidase_supplemented_patients_ L2 - http://dx.doi.org/10.1097/mpg.0b013e318180cd09 DB - PRIME DP - Unbound Medicine ER -