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Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells.
Anticancer Res. 2009 Jan; 29(1):283-9.AR

Abstract

BACKGROUND

Grape seed procyanidins (GSP) can inhibit cell proliferation and tumorigenesis, and induce apoptosis in human breast, prostate, skin and colorectal carcinoma cell lines.

MATERIALS AND METHODS

In order to study the mechanism of apoptosis, four colorectal cell lines, HT-29, SW-480, LoVo and Colo 320DM, were used. GSP-treated cells were assessed for viability by trypan blue exclusion, for loss of mitochondrial membrane potential by rhodamine 123 staining, for increased apoptosis by annexin V labeling, and for changes in the levels of proteins involved in apoptosis by immunoblotting.

RESULTS

GSP had no significant pro-apoptotic effect on the Colo 320DM cell line. In HT-29, SW-480 and LoVo cells, GSP (12.5-50 mg/l) inhibited proliferation in a dose-dependent manner. In these three lines, GSP treatment increased the proportion of rhodamine 123-negative cells and annexin V-positive cells, while immunoblotting revealed increased levels of apoptosis activation protein, caspase-3 and the cleavage fragment of PARP (a caspase-3 substrate), but the level of Bcl-2 did not change.

CONCLUSION

GSP inhibited the proliferation of some colorectal carcinoma cell lines and was associated with an apoptotic mechanism involving a loss of mitochondrial membrane potential and caspase-3 activation in these cells.

Authors+Show Affiliations

Department of Medical Technology, Yuanpei University, Hsinchu, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19331163

Citation

Hsu, Chih-Ping, et al. "Mechanisms of Grape Seed Procyanidin-induced Apoptosis in Colorectal Carcinoma Cells." Anticancer Research, vol. 29, no. 1, 2009, pp. 283-9.
Hsu CP, Lin YH, Chou CC, et al. Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells. Anticancer Res. 2009;29(1):283-9.
Hsu, C. P., Lin, Y. H., Chou, C. C., Zhou, S. P., Hsu, Y. C., Liu, C. L., Ku, F. M., & Chung, Y. C. (2009). Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells. Anticancer Research, 29(1), 283-9.
Hsu CP, et al. Mechanisms of Grape Seed Procyanidin-induced Apoptosis in Colorectal Carcinoma Cells. Anticancer Res. 2009;29(1):283-9. PubMed PMID: 19331163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms of grape seed procyanidin-induced apoptosis in colorectal carcinoma cells. AU - Hsu,Chih-Ping, AU - Lin,Ying-His, AU - Chou,Chih-Chung, AU - Zhou,Shi-Ping, AU - Hsu,Ya-chun, AU - Liu,Chia-Ling, AU - Ku,Fu-Man, AU - Chung,Yuan-Chiang, PY - 2009/4/1/entrez PY - 2009/4/1/pubmed PY - 2009/4/17/medline SP - 283 EP - 9 JF - Anticancer research JO - Anticancer Res VL - 29 IS - 1 N2 - BACKGROUND: Grape seed procyanidins (GSP) can inhibit cell proliferation and tumorigenesis, and induce apoptosis in human breast, prostate, skin and colorectal carcinoma cell lines. MATERIALS AND METHODS: In order to study the mechanism of apoptosis, four colorectal cell lines, HT-29, SW-480, LoVo and Colo 320DM, were used. GSP-treated cells were assessed for viability by trypan blue exclusion, for loss of mitochondrial membrane potential by rhodamine 123 staining, for increased apoptosis by annexin V labeling, and for changes in the levels of proteins involved in apoptosis by immunoblotting. RESULTS: GSP had no significant pro-apoptotic effect on the Colo 320DM cell line. In HT-29, SW-480 and LoVo cells, GSP (12.5-50 mg/l) inhibited proliferation in a dose-dependent manner. In these three lines, GSP treatment increased the proportion of rhodamine 123-negative cells and annexin V-positive cells, while immunoblotting revealed increased levels of apoptosis activation protein, caspase-3 and the cleavage fragment of PARP (a caspase-3 substrate), but the level of Bcl-2 did not change. CONCLUSION: GSP inhibited the proliferation of some colorectal carcinoma cell lines and was associated with an apoptotic mechanism involving a loss of mitochondrial membrane potential and caspase-3 activation in these cells. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/19331163/Mechanisms_of_grape_seed_procyanidin_induced_apoptosis_in_colorectal_carcinoma_cells_ L2 - http://ar.iiarjournals.org/cgi/pmidlookup?view=long&pmid=19331163 DB - PRIME DP - Unbound Medicine ER -