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Galleria mellonella as a model system to study Acinetobacter baumannii pathogenesis and therapeutics.
Antimicrob Agents Chemother. 2009 Jun; 53(6):2605-9.AA

Abstract

Nonmammalian model systems of infection such as Galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. In this study, we utilize G. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism Acinetobacter baumannii and determine the utility of this infection model to study antibacterial efficacy. After infecting G. mellonella caterpillars with a reference A. baumannii strain, we observed that the rate of G. mellonella killing was dependent on the infection inoculum and the incubation temperature postinfection, with greater killing at 37 degrees C than at 30 degrees C (P = 0.01). A. baumannii strains caused greater killing than the less-pathogenic species Acinetobacter baylyi and Acinetobacter lwoffii (P < 0.001). Community-acquired A. baumannii caused greater killing than a reference hospital-acquired strain (P < 0.01). Reduced levels of production of the quorum-sensing molecule 3-hydroxy-C(12)-homoserine lactone caused no change in A. baumannii virulence against G. mellonella. Treatment of a lethal A. baumannii infection with antibiotics that had in vitro activity against the infecting A. baumannii strain significantly prolonged the survival of G. mellonella caterpillars compared with treatment with antibiotics to which the bacteria were resistant. G. mellonella is a relatively simple, nonmammalian model system that can be used to facilitate the in vivo study of host-pathogen interactions in A. baumannii and the efficacy of antibacterial agents.

Authors+Show Affiliations

Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA. apeleg@bidmc.harvard.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19332683

Citation

Peleg, Anton Y., et al. "Galleria Mellonella as a Model System to Study Acinetobacter Baumannii Pathogenesis and Therapeutics." Antimicrobial Agents and Chemotherapy, vol. 53, no. 6, 2009, pp. 2605-9.
Peleg AY, Jara S, Monga D, et al. Galleria mellonella as a model system to study Acinetobacter baumannii pathogenesis and therapeutics. Antimicrob Agents Chemother. 2009;53(6):2605-9.
Peleg, A. Y., Jara, S., Monga, D., Eliopoulos, G. M., Moellering, R. C., & Mylonakis, E. (2009). Galleria mellonella as a model system to study Acinetobacter baumannii pathogenesis and therapeutics. Antimicrobial Agents and Chemotherapy, 53(6), 2605-9. https://doi.org/10.1128/AAC.01533-08
Peleg AY, et al. Galleria Mellonella as a Model System to Study Acinetobacter Baumannii Pathogenesis and Therapeutics. Antimicrob Agents Chemother. 2009;53(6):2605-9. PubMed PMID: 19332683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Galleria mellonella as a model system to study Acinetobacter baumannii pathogenesis and therapeutics. AU - Peleg,Anton Y, AU - Jara,Sebastian, AU - Monga,Divya, AU - Eliopoulos,George M, AU - Moellering,Robert C,Jr AU - Mylonakis,Eleftherios, Y1 - 2009/03/30/ PY - 2009/4/1/entrez PY - 2009/4/1/pubmed PY - 2009/6/26/medline SP - 2605 EP - 9 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 53 IS - 6 N2 - Nonmammalian model systems of infection such as Galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. In this study, we utilize G. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism Acinetobacter baumannii and determine the utility of this infection model to study antibacterial efficacy. After infecting G. mellonella caterpillars with a reference A. baumannii strain, we observed that the rate of G. mellonella killing was dependent on the infection inoculum and the incubation temperature postinfection, with greater killing at 37 degrees C than at 30 degrees C (P = 0.01). A. baumannii strains caused greater killing than the less-pathogenic species Acinetobacter baylyi and Acinetobacter lwoffii (P < 0.001). Community-acquired A. baumannii caused greater killing than a reference hospital-acquired strain (P < 0.01). Reduced levels of production of the quorum-sensing molecule 3-hydroxy-C(12)-homoserine lactone caused no change in A. baumannii virulence against G. mellonella. Treatment of a lethal A. baumannii infection with antibiotics that had in vitro activity against the infecting A. baumannii strain significantly prolonged the survival of G. mellonella caterpillars compared with treatment with antibiotics to which the bacteria were resistant. G. mellonella is a relatively simple, nonmammalian model system that can be used to facilitate the in vivo study of host-pathogen interactions in A. baumannii and the efficacy of antibacterial agents. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/19332683/Galleria_mellonella_as_a_model_system_to_study_Acinetobacter_baumannii_pathogenesis_and_therapeutics_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&amp;pmid=19332683 DB - PRIME DP - Unbound Medicine ER -