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Regulation of tight junction permeability by sodium caprate in human keratinocytes and reconstructed epidermis.
Biochem Biophys Res Commun. 2009 Apr 03; 381(2):171-5.BB

Abstract

Tight junctions (TJs) restrict paracellular flux of water and solutes in epithelia and endothelia. In epidermis, the physiological role of TJs is not fully understood. In this study, sodium caprate (C10), which dilates intestinal TJs, was applied to cultured human epidermal keratinocytes and reconstructed human epidermis to investigate the effects of C10 on epidermal TJs. C10 treatment decreased transepithelial electrical resistance and increased paracellular permeability, although Western blots showed that the expression of TJ-related transmembrane proteins was not decreased. The effects of C10 were reversible. Immunofluorescence microscopy and immuno-replica electron microscopy showed that the localization of TJ strands were disintegrated, concomitant with the dispersion and/or disappearance of TJ-related molecules from the cell surface. These findings suggest that C10 impairs barrier function by physically disrupting TJ conformation in the epidermis. Furthermore, these results also show that proper localization of the molecules on the cellular membrane is important for TJ barrier function.

Authors+Show Affiliations

Pola Chemical Industries Inc., 560 Kashio-cho, Totsuka-ku, Yokohama 244-0812, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19338770

Citation

Kurasawa, Masumi, et al. "Regulation of Tight Junction Permeability By Sodium Caprate in Human Keratinocytes and Reconstructed Epidermis." Biochemical and Biophysical Research Communications, vol. 381, no. 2, 2009, pp. 171-5.
Kurasawa M, Kuroda S, Kida N, et al. Regulation of tight junction permeability by sodium caprate in human keratinocytes and reconstructed epidermis. Biochem Biophys Res Commun. 2009;381(2):171-5.
Kurasawa, M., Kuroda, S., Kida, N., Murata, M., Oba, A., Yamamoto, T., & Sasaki, H. (2009). Regulation of tight junction permeability by sodium caprate in human keratinocytes and reconstructed epidermis. Biochemical and Biophysical Research Communications, 381(2), 171-5. https://doi.org/10.1016/j.bbrc.2009.02.005
Kurasawa M, et al. Regulation of Tight Junction Permeability By Sodium Caprate in Human Keratinocytes and Reconstructed Epidermis. Biochem Biophys Res Commun. 2009 Apr 3;381(2):171-5. PubMed PMID: 19338770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of tight junction permeability by sodium caprate in human keratinocytes and reconstructed epidermis. AU - Kurasawa,Masumi, AU - Kuroda,Shohei, AU - Kida,Naoko, AU - Murata,Michiyo, AU - Oba,Ai, AU - Yamamoto,Takuya, AU - Sasaki,Hiroyuki, Y1 - 2009/02/10/ PY - 2009/02/02/received PY - 2009/02/05/accepted PY - 2009/4/3/entrez PY - 2009/4/3/pubmed PY - 2009/4/21/medline SP - 171 EP - 5 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 381 IS - 2 N2 - Tight junctions (TJs) restrict paracellular flux of water and solutes in epithelia and endothelia. In epidermis, the physiological role of TJs is not fully understood. In this study, sodium caprate (C10), which dilates intestinal TJs, was applied to cultured human epidermal keratinocytes and reconstructed human epidermis to investigate the effects of C10 on epidermal TJs. C10 treatment decreased transepithelial electrical resistance and increased paracellular permeability, although Western blots showed that the expression of TJ-related transmembrane proteins was not decreased. The effects of C10 were reversible. Immunofluorescence microscopy and immuno-replica electron microscopy showed that the localization of TJ strands were disintegrated, concomitant with the dispersion and/or disappearance of TJ-related molecules from the cell surface. These findings suggest that C10 impairs barrier function by physically disrupting TJ conformation in the epidermis. Furthermore, these results also show that proper localization of the molecules on the cellular membrane is important for TJ barrier function. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/19338770/Regulation_of_tight_junction_permeability_by_sodium_caprate_in_human_keratinocytes_and_reconstructed_epidermis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(09)00260-5 DB - PRIME DP - Unbound Medicine ER -