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Suppressive effects of ginsan on the development of allergic reaction in murine asthmatic model.
Int Arch Allergy Immunol. 2009; 150(1):32-42.IA

Abstract

BACKGROUND

Asthma is a major health problem worldwide, and the morbidity and mortality caused by asthma are on the rise. Corticosteroid therapies for asthma treatment frequently induce many side effects. Therefore, the development of new medicines that have both high efficacy and fewer side effects has been a scientific challenge. Here we tested the effect of ginsan, a polysaccharide derived from Panax ginseng, against allergic reaction in an ovalbumin (OVA)-induced murine asthmatic model in comparison with dexamethasone, and investigated its underlying mechanism.

METHODS

To induce murine asthma, mice were sensitized and challenged with OVA. Ginsan or dexamethasone was administered by injection 3 times a week. Airway hyperresponsiveness, airway inflammation and lung pathology were assessed in order to evaluate the effect of ginsan against asthma.

RESULTS

Ginsan treatment reduced airway hyperresponsiveness, remodeling and eosinophilia. These effects of ginsan were equivalent to those of dexamethasone. Ginsan treatment decreased the IL-5 level in the supernatant of cultured splenocytes, while IFN-gamma and serum IgE were not altered. To elucidate the mechanism of ginsan, expression of inflammation-related genes were screened. Interestingly, ginsan treatment upregulated cyclooxygenase (COX)-1 and COX-2 mRNA, and expression of their proteins in the lung were also increased. PGE(2) in the bronchoalveolar lavage fluid was also increased by the ginsan treatment. Lastly, ginsan inhibited the allergic reaction aggravated by COX inhibitor (indomethacin).

CONCLUSION

Ginsan has anti-asthmatic effects, which seem to be partially mediated by enhancing the synthesis of COX gene products.

Authors+Show Affiliations

Department of Microbiology, Chonnam National University Medical School, Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University, Gwangju 501-746, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19339800

Citation

Lim, You-Jin, et al. "Suppressive Effects of Ginsan On the Development of Allergic Reaction in Murine Asthmatic Model." International Archives of Allergy and Immunology, vol. 150, no. 1, 2009, pp. 32-42.
Lim YJ, Na HS, Yun YS, et al. Suppressive effects of ginsan on the development of allergic reaction in murine asthmatic model. Int Arch Allergy Immunol. 2009;150(1):32-42.
Lim, Y. J., Na, H. S., Yun, Y. S., Choi, I. S., Oh, J. S., Rhee, J. H., Cho, B. H., & Lee, H. C. (2009). Suppressive effects of ginsan on the development of allergic reaction in murine asthmatic model. International Archives of Allergy and Immunology, 150(1), 32-42. https://doi.org/10.1159/000210378
Lim YJ, et al. Suppressive Effects of Ginsan On the Development of Allergic Reaction in Murine Asthmatic Model. Int Arch Allergy Immunol. 2009;150(1):32-42. PubMed PMID: 19339800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppressive effects of ginsan on the development of allergic reaction in murine asthmatic model. AU - Lim,You-Jin, AU - Na,Hee-Sam, AU - Yun,Yeon-Sook, AU - Choi,Inseon S, AU - Oh,Jong-Suk, AU - Rhee,Joon-Haeng, AU - Cho,Bok-Hee, AU - Lee,Hyun-Chul, Y1 - 2009/04/02/ PY - 2008/01/17/received PY - 2008/12/16/accepted PY - 2009/4/3/entrez PY - 2009/4/3/pubmed PY - 2009/9/5/medline SP - 32 EP - 42 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 150 IS - 1 N2 - BACKGROUND: Asthma is a major health problem worldwide, and the morbidity and mortality caused by asthma are on the rise. Corticosteroid therapies for asthma treatment frequently induce many side effects. Therefore, the development of new medicines that have both high efficacy and fewer side effects has been a scientific challenge. Here we tested the effect of ginsan, a polysaccharide derived from Panax ginseng, against allergic reaction in an ovalbumin (OVA)-induced murine asthmatic model in comparison with dexamethasone, and investigated its underlying mechanism. METHODS: To induce murine asthma, mice were sensitized and challenged with OVA. Ginsan or dexamethasone was administered by injection 3 times a week. Airway hyperresponsiveness, airway inflammation and lung pathology were assessed in order to evaluate the effect of ginsan against asthma. RESULTS: Ginsan treatment reduced airway hyperresponsiveness, remodeling and eosinophilia. These effects of ginsan were equivalent to those of dexamethasone. Ginsan treatment decreased the IL-5 level in the supernatant of cultured splenocytes, while IFN-gamma and serum IgE were not altered. To elucidate the mechanism of ginsan, expression of inflammation-related genes were screened. Interestingly, ginsan treatment upregulated cyclooxygenase (COX)-1 and COX-2 mRNA, and expression of their proteins in the lung were also increased. PGE(2) in the bronchoalveolar lavage fluid was also increased by the ginsan treatment. Lastly, ginsan inhibited the allergic reaction aggravated by COX inhibitor (indomethacin). CONCLUSION: Ginsan has anti-asthmatic effects, which seem to be partially mediated by enhancing the synthesis of COX gene products. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/19339800/Suppressive_effects_of_ginsan_on_the_development_of_allergic_reaction_in_murine_asthmatic_model_ DB - PRIME DP - Unbound Medicine ER -