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Effect of nutritional vitamin A deficiency on lipid metabolism in the rat heart: Its relation to PPAR gene expression.
Nutrition. 2009 Jul-Aug; 25(7-8):828-38.N

Abstract

OBJECTIVE

We studied the effect of dietary vitamin A deprivation on lipid composition and mRNA expression of regulatory enzymes involved in rat heart energetic lipid metabolism and its relation to the expression of peroxisome proliferator-activated receptor (PPAR) and retinoid X receptor (RXR) genes.

METHODS

Male Wistar 21-d-old rats were fed for 3 mo with a vitamin A-free diet (vitamin A-deficient group) and the same diet plus 8 mg of retinol palmitate per kilogram of diet (control group). One group of deficient animals received the control diet 15 d before sacrifice (vitamin A-refed group). Heart ventricular and mitochondrial lipid contents were determined. Lipid synthesis was measured using radioactive precursors and acetyl-coenzyme A carboxylase and mitochondrial carnitine palmitoyltransferase-I (CPT-I) activities using radioactive substrates. Fatty acid composition of mitochondrial phospholipids was analyzed by gas-liquid chromatography. Heart expression of acetyl-coenzyme A carboxylase, CPT-I, PPAR-alpha, PPAR-beta, RXR-alpha, and RXR-beta was assessed by reverse transcriptase polymerase chain reaction, and CPT-I expression was also measured by real-time polymerase chain reaction.

RESULTS

Vitamin A deficiency induced changes in heart ventricular lipid content and synthesis. Mitochondrial cardiolipin decreased and the proportion of phospholipids/saturated fatty acids increased. Heart activity and mRNA levels of CPT-I and expression of PPAR-alpha and PPAR-beta genes were enhanced, whereas acetyl-coenzyme A carboxylase activity diminished. Furthermore, vitamin A deficiency decreased heart mRNA levels of RXRs. Vitamin A refeeding reverted most of the observed changes.

CONCLUSION

Lipid metabolism is significantly modified in hearts of vitamin A-deficient rats. Alteration of mitochondrial energetic processes by modifying the activity and gene expressions of the regulatory enzymes is associated with a high PPAR expression induced by vitamin A deprivation.

Authors+Show Affiliations

Department of Biochemistry and Biological Sciences, National University of San Luis, and IMIBIO-SL, CONICET, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19342198

Citation

Vega, Verónica Analía, et al. "Effect of Nutritional Vitamin a Deficiency On Lipid Metabolism in the Rat Heart: Its Relation to PPAR Gene Expression." Nutrition (Burbank, Los Angeles County, Calif.), vol. 25, no. 7-8, 2009, pp. 828-38.
Vega VA, Anzulovich AC, Varas SM, et al. Effect of nutritional vitamin A deficiency on lipid metabolism in the rat heart: Its relation to PPAR gene expression. Nutrition. 2009;25(7-8):828-38.
Vega, V. A., Anzulovich, A. C., Varas, S. M., Bonomi, M. R., Giménez, M. S., & Oliveros, L. B. (2009). Effect of nutritional vitamin A deficiency on lipid metabolism in the rat heart: Its relation to PPAR gene expression. Nutrition (Burbank, Los Angeles County, Calif.), 25(7-8), 828-38. https://doi.org/10.1016/j.nut.2009.01.008
Vega VA, et al. Effect of Nutritional Vitamin a Deficiency On Lipid Metabolism in the Rat Heart: Its Relation to PPAR Gene Expression. Nutrition. 2009 Jul-Aug;25(7-8):828-38. PubMed PMID: 19342198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of nutritional vitamin A deficiency on lipid metabolism in the rat heart: Its relation to PPAR gene expression. AU - Vega,Verónica Analía, AU - Anzulovich,Ana Cecilia, AU - Varas,Silvia Mabel, AU - Bonomi,Mirtha Ruth, AU - Giménez,María Sofía, AU - Oliveros,Liliana Beatriz, Y1 - 2009/04/01/ PY - 2008/09/29/received PY - 2008/12/27/revised PY - 2009/01/05/accepted PY - 2009/4/4/entrez PY - 2009/4/4/pubmed PY - 2009/10/21/medline SP - 828 EP - 38 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 25 IS - 7-8 N2 - OBJECTIVE: We studied the effect of dietary vitamin A deprivation on lipid composition and mRNA expression of regulatory enzymes involved in rat heart energetic lipid metabolism and its relation to the expression of peroxisome proliferator-activated receptor (PPAR) and retinoid X receptor (RXR) genes. METHODS: Male Wistar 21-d-old rats were fed for 3 mo with a vitamin A-free diet (vitamin A-deficient group) and the same diet plus 8 mg of retinol palmitate per kilogram of diet (control group). One group of deficient animals received the control diet 15 d before sacrifice (vitamin A-refed group). Heart ventricular and mitochondrial lipid contents were determined. Lipid synthesis was measured using radioactive precursors and acetyl-coenzyme A carboxylase and mitochondrial carnitine palmitoyltransferase-I (CPT-I) activities using radioactive substrates. Fatty acid composition of mitochondrial phospholipids was analyzed by gas-liquid chromatography. Heart expression of acetyl-coenzyme A carboxylase, CPT-I, PPAR-alpha, PPAR-beta, RXR-alpha, and RXR-beta was assessed by reverse transcriptase polymerase chain reaction, and CPT-I expression was also measured by real-time polymerase chain reaction. RESULTS: Vitamin A deficiency induced changes in heart ventricular lipid content and synthesis. Mitochondrial cardiolipin decreased and the proportion of phospholipids/saturated fatty acids increased. Heart activity and mRNA levels of CPT-I and expression of PPAR-alpha and PPAR-beta genes were enhanced, whereas acetyl-coenzyme A carboxylase activity diminished. Furthermore, vitamin A deficiency decreased heart mRNA levels of RXRs. Vitamin A refeeding reverted most of the observed changes. CONCLUSION: Lipid metabolism is significantly modified in hearts of vitamin A-deficient rats. Alteration of mitochondrial energetic processes by modifying the activity and gene expressions of the regulatory enzymes is associated with a high PPAR expression induced by vitamin A deprivation. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/19342198/Effect_of_nutritional_vitamin_A_deficiency_on_lipid_metabolism_in_the_rat_heart:_Its_relation_to_PPAR_gene_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(09)00052-5 DB - PRIME DP - Unbound Medicine ER -