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Usefulness of human telomerase reverse transcriptase in pancreatic juice as a biomarker of pancreatic malignancy.
Pancreas. 2009 Jul; 38(5):527-33.P

Abstract

OBJECTIVES

Human telomerase reverse transcriptase (hTERT), one of the subunits of telomerase, is a promising diagnostic marker for pancreatic cancer. In the present study, we did a large-scale analysis of 115 preoperative pancreatic juice specimens to evaluate the feasibility of detection of hTERT expression by immunohistochemistry for preoperative diagnosis of pancreatic malignancy.

METHODS

The expression of hTERT was examined by immunohistochemistry in preoperative pancreatic juice samples.

RESULTS

In pancreatic juice samples, hTERT expression was detectable in 84% of pancreatic ductal adenocarcinomas (PDACs), whereas 62% of PDACs were positive by cytology. In intraductal papillary mucinous neoplasms (IPMNs), hTERT expression was detectable in 88% of malignant IPMNs, whereas only 22% were positive by cytology. The sensitivity, specificity, and overall accuracy of hTERT expression for differentiation between carcinoma and other benign diseases were 85.1%, 82.1%, and 84.3%, respectively, whereas the same values for cytologic accuracy were 47.1%, 89.3%, and 57.4%, respectively. When the results of cytology and hTERT expression were combined, the sensitivity and overall accuracy increased to 92.0% and 87.8%, respectively.

CONCLUSIONS

Our results suggested that the assessment of hTERT expression in preoperative pancreatic juice increased the sensitivity and accuracy of diagnosis of PDACs and malignant IPMNs without using special techniques.

Authors+Show Affiliations

Division of Clinical Medical Science, Department of Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Minami-Ku, Hiroshima, Japan. anakashima@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19342980

Citation

Nakashima, Akira, et al. "Usefulness of Human Telomerase Reverse Transcriptase in Pancreatic Juice as a Biomarker of Pancreatic Malignancy." Pancreas, vol. 38, no. 5, 2009, pp. 527-33.
Nakashima A, Murakami Y, Uemura K, et al. Usefulness of human telomerase reverse transcriptase in pancreatic juice as a biomarker of pancreatic malignancy. Pancreas. 2009;38(5):527-33.
Nakashima, A., Murakami, Y., Uemura, K., Hayashidani, Y., Sudo, T., Hashimoto, Y., Ohge, H., Oda, M., Sueda, T., & Hiyama, E. (2009). Usefulness of human telomerase reverse transcriptase in pancreatic juice as a biomarker of pancreatic malignancy. Pancreas, 38(5), 527-33. https://doi.org/10.1097/MPA.0b013e3181a16d28
Nakashima A, et al. Usefulness of Human Telomerase Reverse Transcriptase in Pancreatic Juice as a Biomarker of Pancreatic Malignancy. Pancreas. 2009;38(5):527-33. PubMed PMID: 19342980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usefulness of human telomerase reverse transcriptase in pancreatic juice as a biomarker of pancreatic malignancy. AU - Nakashima,Akira, AU - Murakami,Yoshiaki, AU - Uemura,Kenichiro, AU - Hayashidani,Yasuo, AU - Sudo,Takeshi, AU - Hashimoto,Yasushi, AU - Ohge,Hiroki, AU - Oda,Miyo, AU - Sueda,Taijiro, AU - Hiyama,Eiso, PY - 2009/4/4/entrez PY - 2009/4/4/pubmed PY - 2009/9/26/medline SP - 527 EP - 33 JF - Pancreas JO - Pancreas VL - 38 IS - 5 N2 - OBJECTIVES: Human telomerase reverse transcriptase (hTERT), one of the subunits of telomerase, is a promising diagnostic marker for pancreatic cancer. In the present study, we did a large-scale analysis of 115 preoperative pancreatic juice specimens to evaluate the feasibility of detection of hTERT expression by immunohistochemistry for preoperative diagnosis of pancreatic malignancy. METHODS: The expression of hTERT was examined by immunohistochemistry in preoperative pancreatic juice samples. RESULTS: In pancreatic juice samples, hTERT expression was detectable in 84% of pancreatic ductal adenocarcinomas (PDACs), whereas 62% of PDACs were positive by cytology. In intraductal papillary mucinous neoplasms (IPMNs), hTERT expression was detectable in 88% of malignant IPMNs, whereas only 22% were positive by cytology. The sensitivity, specificity, and overall accuracy of hTERT expression for differentiation between carcinoma and other benign diseases were 85.1%, 82.1%, and 84.3%, respectively, whereas the same values for cytologic accuracy were 47.1%, 89.3%, and 57.4%, respectively. When the results of cytology and hTERT expression were combined, the sensitivity and overall accuracy increased to 92.0% and 87.8%, respectively. CONCLUSIONS: Our results suggested that the assessment of hTERT expression in preoperative pancreatic juice increased the sensitivity and accuracy of diagnosis of PDACs and malignant IPMNs without using special techniques. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/19342980/Usefulness_of_human_telomerase_reverse_transcriptase_in_pancreatic_juice_as_a_biomarker_of_pancreatic_malignancy_ L2 - http://dx.doi.org/10.1097/MPA.0b013e3181a16d28 DB - PRIME DP - Unbound Medicine ER -