Tags

Type your tag names separated by a space and hit enter

Proteome of plant peroxisomes: new perspectives on the role of these organelles in cell biology.
Proteomics. 2009 May; 9(9):2301-12.P

Abstract

Peroxisomes are cell organelles bounded by a single membrane with a basically oxidative metabolism. Peroxisomes house catalase and H(2)O(2)-producing flavin-oxidases as the main protein constituents. However, since their discovery in early fifties, a number of new enzymes and metabolic pathways have been reported to be also confined to these organelles. Thus, the presence of exo- and endo-peptidases, superoxide dismutases, the enzymes of the plant ascorbate-glutathione cycle plus ascorbate and glutathione, several NADP-dehydrogenases, and also L-arginine-dependent nitric oxide synthase activity has evidenced the relevant role of these organelles in cell physiology. In recent years, the study of new functions of peroxisomes has become a field of intensive research in cell biology, and these organelles have been proposed to be a source of important signal molecules for different transduction pathways. In plants, peroxisomes participate in seed germination, leaf senescence, fruit maturation, response to abiotic and biotic stress, photomorphogenesis, biosynthesis of the plant hormones jasmonic acid and auxin, and in cell signaling by reactive oxygen and nitrogen species (ROS and RNS, respectively). In order to decipher the nature and specific role of the peroxisomal proteins in these processes, several approaches including in vivo and in vitro import assays and generation of mutants have been used. In the last decade, the development of genomics and the report of the first plant genomes provided plant biologists a powerful tool to assign to peroxisomes those proteins which harbored any of the two peroxisomal targeting signals (PTS, either PTS1 or PTS2) described so far. Unfortunately, those molecular approaches could not give any response to those proteins previously localized in plant peroxisomes by classical biochemical and cell biology methods that did not contain any PTS. However, more recently, proteomic studies of highly purified organelles have provided evidence of the presence in peroxisomes of new proteins not previously reported. Thus, the contribution of proteomic approaches to the biology of peroxisomes is essential, not only for elucidation of the mechanisms involved in the import of the PTS1- and PTS2-independent proteins, but also to the understanding of the role of these organelles in the cell physiology of plant growth and development.

Authors+Show Affiliations

Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain. jmpalma@eez.csic.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19343723

Citation

Palma, José M., et al. "Proteome of Plant Peroxisomes: New Perspectives On the Role of These Organelles in Cell Biology." Proteomics, vol. 9, no. 9, 2009, pp. 2301-12.
Palma JM, Corpas FJ, del Río LA. Proteome of plant peroxisomes: new perspectives on the role of these organelles in cell biology. Proteomics. 2009;9(9):2301-12.
Palma, J. M., Corpas, F. J., & del Río, L. A. (2009). Proteome of plant peroxisomes: new perspectives on the role of these organelles in cell biology. Proteomics, 9(9), 2301-12. https://doi.org/10.1002/pmic.200700732
Palma JM, Corpas FJ, del Río LA. Proteome of Plant Peroxisomes: New Perspectives On the Role of These Organelles in Cell Biology. Proteomics. 2009;9(9):2301-12. PubMed PMID: 19343723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteome of plant peroxisomes: new perspectives on the role of these organelles in cell biology. AU - Palma,José M, AU - Corpas,Francisco J, AU - del Río,Luis A, PY - 2009/4/4/entrez PY - 2009/4/4/pubmed PY - 2009/8/28/medline SP - 2301 EP - 12 JF - Proteomics JO - Proteomics VL - 9 IS - 9 N2 - Peroxisomes are cell organelles bounded by a single membrane with a basically oxidative metabolism. Peroxisomes house catalase and H(2)O(2)-producing flavin-oxidases as the main protein constituents. However, since their discovery in early fifties, a number of new enzymes and metabolic pathways have been reported to be also confined to these organelles. Thus, the presence of exo- and endo-peptidases, superoxide dismutases, the enzymes of the plant ascorbate-glutathione cycle plus ascorbate and glutathione, several NADP-dehydrogenases, and also L-arginine-dependent nitric oxide synthase activity has evidenced the relevant role of these organelles in cell physiology. In recent years, the study of new functions of peroxisomes has become a field of intensive research in cell biology, and these organelles have been proposed to be a source of important signal molecules for different transduction pathways. In plants, peroxisomes participate in seed germination, leaf senescence, fruit maturation, response to abiotic and biotic stress, photomorphogenesis, biosynthesis of the plant hormones jasmonic acid and auxin, and in cell signaling by reactive oxygen and nitrogen species (ROS and RNS, respectively). In order to decipher the nature and specific role of the peroxisomal proteins in these processes, several approaches including in vivo and in vitro import assays and generation of mutants have been used. In the last decade, the development of genomics and the report of the first plant genomes provided plant biologists a powerful tool to assign to peroxisomes those proteins which harbored any of the two peroxisomal targeting signals (PTS, either PTS1 or PTS2) described so far. Unfortunately, those molecular approaches could not give any response to those proteins previously localized in plant peroxisomes by classical biochemical and cell biology methods that did not contain any PTS. However, more recently, proteomic studies of highly purified organelles have provided evidence of the presence in peroxisomes of new proteins not previously reported. Thus, the contribution of proteomic approaches to the biology of peroxisomes is essential, not only for elucidation of the mechanisms involved in the import of the PTS1- and PTS2-independent proteins, but also to the understanding of the role of these organelles in the cell physiology of plant growth and development. SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/19343723/Proteome_of_plant_peroxisomes:_new_perspectives_on_the_role_of_these_organelles_in_cell_biology_ L2 - https://doi.org/10.1002/pmic.200700732 DB - PRIME DP - Unbound Medicine ER -