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Infrared spectroscopy studies of mixtures prepared with synthetic ceramides varying in head group architecture: coexistence of liquid and crystalline phases.
Biochim Biophys Acta. 2009 Mar; 1788(3):732-42.BB

Abstract

The barrier function of the skin is provided by the stratum corneum (SC), the outermost layer of the skin.Ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs) are present in SC and form highly ordered crystalline lipid lamellae. These lamellae are crucial for a proper skin barrier function. In the present study,Fourier transform infrared spectroscopy was used to examine the lipid organization of mixtures prepared from synthetic CERs with CHOL and FFAs. The conformational ordering and lateral packing of these mixtures showed great similarities to the lipid organization in SC and lipid mixtures prepared with native CERs.Therefore, mixtures with synthetic CERs serve as an excellent tool for studying the effect of molecular architecture of CER subclasses on the lipid phase behavior. In SC the number of OH-groups in the head groups of CER subclasses varies. Furthermore, acylCERs with a linoleic acid chemically bound to a long acyl chain are also identified. The present study revealed that CER head group architecture affects the lateral packing and conformational ordering of the CER:CHOL:FFA mixtures. Furthermore, while the majority of the lipids form a crystalline packing, the linoleate moiety of the acylCERs participates in a "pseudo fluid" phase.

Authors+Show Affiliations

Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19344626

Citation

Janssens, M, et al. "Infrared Spectroscopy Studies of Mixtures Prepared With Synthetic Ceramides Varying in Head Group Architecture: Coexistence of Liquid and Crystalline Phases." Biochimica Et Biophysica Acta, vol. 1788, no. 3, 2009, pp. 732-42.
Janssens M, Gooris GS, Bouwstra JA. Infrared spectroscopy studies of mixtures prepared with synthetic ceramides varying in head group architecture: coexistence of liquid and crystalline phases. Biochim Biophys Acta. 2009;1788(3):732-42.
Janssens, M., Gooris, G. S., & Bouwstra, J. A. (2009). Infrared spectroscopy studies of mixtures prepared with synthetic ceramides varying in head group architecture: coexistence of liquid and crystalline phases. Biochimica Et Biophysica Acta, 1788(3), 732-42. https://doi.org/10.1016/j.bbamem.2009.01.003
Janssens M, Gooris GS, Bouwstra JA. Infrared Spectroscopy Studies of Mixtures Prepared With Synthetic Ceramides Varying in Head Group Architecture: Coexistence of Liquid and Crystalline Phases. Biochim Biophys Acta. 2009;1788(3):732-42. PubMed PMID: 19344626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Infrared spectroscopy studies of mixtures prepared with synthetic ceramides varying in head group architecture: coexistence of liquid and crystalline phases. AU - Janssens,M, AU - Gooris,G S, AU - Bouwstra,J A, PY - 2008/10/08/received PY - 2008/12/01/revised PY - 2009/01/13/accepted PY - 2009/4/7/entrez PY - 2009/4/7/pubmed PY - 2009/5/15/medline SP - 732 EP - 42 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1788 IS - 3 N2 - The barrier function of the skin is provided by the stratum corneum (SC), the outermost layer of the skin.Ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs) are present in SC and form highly ordered crystalline lipid lamellae. These lamellae are crucial for a proper skin barrier function. In the present study,Fourier transform infrared spectroscopy was used to examine the lipid organization of mixtures prepared from synthetic CERs with CHOL and FFAs. The conformational ordering and lateral packing of these mixtures showed great similarities to the lipid organization in SC and lipid mixtures prepared with native CERs.Therefore, mixtures with synthetic CERs serve as an excellent tool for studying the effect of molecular architecture of CER subclasses on the lipid phase behavior. In SC the number of OH-groups in the head groups of CER subclasses varies. Furthermore, acylCERs with a linoleic acid chemically bound to a long acyl chain are also identified. The present study revealed that CER head group architecture affects the lateral packing and conformational ordering of the CER:CHOL:FFA mixtures. Furthermore, while the majority of the lipids form a crystalline packing, the linoleate moiety of the acylCERs participates in a "pseudo fluid" phase. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/19344626/Infrared_spectroscopy_studies_of_mixtures_prepared_with_synthetic_ceramides_varying_in_head_group_architecture:_coexistence_of_liquid_and_crystalline_phases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0005-2736(09)00008-X DB - PRIME DP - Unbound Medicine ER -