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Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming.
J Proteomics. 2009 Mar 06; 72(2):165-82.JP

Abstract

Snakebite envenoming represents a neglected tropical disease that has a heavy public health impact, particularly in Asia, Africa and Latin America. A global initiative, aimed at increasing antivenom production and accessibility, is being promoted by the World Health Organization and others. This work discusses several aspects of antivenom manufacture and control in which the proteomic analysis of snake venoms, for which the term 'snake venomics' has been coined, might play a relevant supporting role. Snake venomics has already shown its usefulness for generating knowledge at different levels (ontogenetic, individual, and geographic) on inter- and intraspecies venom variability. This information has applications for the quality control of venom preparations used in antivenom manufacture. Moreover, the design of the best venom mixtures for immunization, aimed at increasing the effectiveness of antivenoms, may also be guided by venom proteome analysis, including molecular studies of the cross-reactivity of antivenoms and heterologous venoms through a recently developed methodological approach termed 'antivenomics'. Results generated by proteomic protocols should be complemented by preclinical testing of antivenom efficacy using functional neutralization assays. Snake venomics might be also helpful in designing alternative in vitro tests for the assessment of antivenom efficacy that would eventually substitute current in vivo tests.

Authors+Show Affiliations

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. jgutierr@icp.ucr.ac.crNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19344652

Citation

Gutiérrez, José María, et al. "Snake Venomics and Antivenomics: Proteomic Tools in the Design and Control of Antivenoms for the Treatment of Snakebite Envenoming." Journal of Proteomics, vol. 72, no. 2, 2009, pp. 165-82.
Gutiérrez JM, Lomonte B, León G, et al. Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming. J Proteomics. 2009;72(2):165-82.
Gutiérrez, J. M., Lomonte, B., León, G., Alape-Girón, A., Flores-Díaz, M., Sanz, L., Angulo, Y., & Calvete, J. J. (2009). Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming. Journal of Proteomics, 72(2), 165-82. https://doi.org/10.1016/j.jprot.2009.01.008
Gutiérrez JM, et al. Snake Venomics and Antivenomics: Proteomic Tools in the Design and Control of Antivenoms for the Treatment of Snakebite Envenoming. J Proteomics. 2009 Mar 6;72(2):165-82. PubMed PMID: 19344652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming. AU - Gutiérrez,José María, AU - Lomonte,Bruno, AU - León,Guillermo, AU - Alape-Girón,Alberto, AU - Flores-Díaz,Marietta, AU - Sanz,Libia, AU - Angulo,Yamileth, AU - Calvete,Juan J, Y1 - 2009/01/21/ PY - 2008/12/23/received PY - 2009/01/01/revised PY - 2009/01/07/accepted PY - 2009/4/7/entrez PY - 2009/4/7/pubmed PY - 2009/7/10/medline SP - 165 EP - 82 JF - Journal of proteomics JO - J Proteomics VL - 72 IS - 2 N2 - Snakebite envenoming represents a neglected tropical disease that has a heavy public health impact, particularly in Asia, Africa and Latin America. A global initiative, aimed at increasing antivenom production and accessibility, is being promoted by the World Health Organization and others. This work discusses several aspects of antivenom manufacture and control in which the proteomic analysis of snake venoms, for which the term 'snake venomics' has been coined, might play a relevant supporting role. Snake venomics has already shown its usefulness for generating knowledge at different levels (ontogenetic, individual, and geographic) on inter- and intraspecies venom variability. This information has applications for the quality control of venom preparations used in antivenom manufacture. Moreover, the design of the best venom mixtures for immunization, aimed at increasing the effectiveness of antivenoms, may also be guided by venom proteome analysis, including molecular studies of the cross-reactivity of antivenoms and heterologous venoms through a recently developed methodological approach termed 'antivenomics'. Results generated by proteomic protocols should be complemented by preclinical testing of antivenom efficacy using functional neutralization assays. Snake venomics might be also helpful in designing alternative in vitro tests for the assessment of antivenom efficacy that would eventually substitute current in vivo tests. SN - 1874-3919 UR - https://www.unboundmedicine.com/medline/citation/19344652/Snake_venomics_and_antivenomics:_Proteomic_tools_in_the_design_and_control_of_antivenoms_for_the_treatment_of_snakebite_envenoming_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(09)00012-8 DB - PRIME DP - Unbound Medicine ER -