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Diversity and morphology of members of the phylum "synergistetes" in periodontal health and disease.
Appl Environ Microbiol. 2009 Jun; 75(11):3777-86.AE

Abstract

Members of the phylum "Synergistetes" have frequently been detected in the human oral cavity at sites of dental disease, but they have rarely been detected in studies of oral health. Only two oral "Synergistetes" taxa are cultivable. The aims of this study were to investigate the diversity of "Synergistetes" in the oral cavity, to establish whether "Synergistetes" taxa are more strongly associated with periodontitis than with oral health, and to visualize unculturable "Synergistetes" in situ. Sixty samples (saliva, dental plaque, and mucosal swabs) were collected from five subjects with periodontitis and five periodontally healthy controls. Using phylum-specific 16S rRNA gene primers, "Synergistetes" were identified by PCR, cloning, and sequencing of 48 clones per PCR-positive sample. Subgingival plaque samples were labeled with probes targeting rRNA of unculturable oral "Synergistetes" using fluorescent in situ hybridization (FISH). Analysis of 1,664 clones revealed 12 "Synergistetes" operational taxonomic units (OTUs) at the 99% sequence identity level, 5 of which were novel. "Synergistetes" OTU 4.2 was found in significantly more subjects with periodontitis than controls (P = 0.048) and was more abundant in subgingival plaque at diseased sites than at healthy sites in subjects with periodontitis (P = 0.019) or controls (P = 0.019). FISH analysis revealed that unculturable oral "Synergistetes" cells were large curved bacilli. The human oral cavity harbors a diverse population of "Synergistetes." "Synergistetes" OTU 4.2 is associated with periodontitis and may have a pathogenic role.

Authors+Show Affiliations

Department of Microbiology, King's College London, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19346352

Citation

Vartoukian, S R., et al. "Diversity and Morphology of Members of the Phylum "synergistetes" in Periodontal Health and Disease." Applied and Environmental Microbiology, vol. 75, no. 11, 2009, pp. 3777-86.
Vartoukian SR, Palmer RM, Wade WG. Diversity and morphology of members of the phylum "synergistetes" in periodontal health and disease. Appl Environ Microbiol. 2009;75(11):3777-86.
Vartoukian, S. R., Palmer, R. M., & Wade, W. G. (2009). Diversity and morphology of members of the phylum "synergistetes" in periodontal health and disease. Applied and Environmental Microbiology, 75(11), 3777-86. https://doi.org/10.1128/AEM.02763-08
Vartoukian SR, Palmer RM, Wade WG. Diversity and Morphology of Members of the Phylum "synergistetes" in Periodontal Health and Disease. Appl Environ Microbiol. 2009;75(11):3777-86. PubMed PMID: 19346352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and morphology of members of the phylum "synergistetes" in periodontal health and disease. AU - Vartoukian,S R, AU - Palmer,R M, AU - Wade,W G, Y1 - 2009/04/03/ PY - 2009/4/7/entrez PY - 2009/4/7/pubmed PY - 2009/6/16/medline SP - 3777 EP - 86 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 75 IS - 11 N2 - Members of the phylum "Synergistetes" have frequently been detected in the human oral cavity at sites of dental disease, but they have rarely been detected in studies of oral health. Only two oral "Synergistetes" taxa are cultivable. The aims of this study were to investigate the diversity of "Synergistetes" in the oral cavity, to establish whether "Synergistetes" taxa are more strongly associated with periodontitis than with oral health, and to visualize unculturable "Synergistetes" in situ. Sixty samples (saliva, dental plaque, and mucosal swabs) were collected from five subjects with periodontitis and five periodontally healthy controls. Using phylum-specific 16S rRNA gene primers, "Synergistetes" were identified by PCR, cloning, and sequencing of 48 clones per PCR-positive sample. Subgingival plaque samples were labeled with probes targeting rRNA of unculturable oral "Synergistetes" using fluorescent in situ hybridization (FISH). Analysis of 1,664 clones revealed 12 "Synergistetes" operational taxonomic units (OTUs) at the 99% sequence identity level, 5 of which were novel. "Synergistetes" OTU 4.2 was found in significantly more subjects with periodontitis than controls (P = 0.048) and was more abundant in subgingival plaque at diseased sites than at healthy sites in subjects with periodontitis (P = 0.019) or controls (P = 0.019). FISH analysis revealed that unculturable oral "Synergistetes" cells were large curved bacilli. The human oral cavity harbors a diverse population of "Synergistetes." "Synergistetes" OTU 4.2 is associated with periodontitis and may have a pathogenic role. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/19346352/Diversity_and_morphology_of_members_of_the_phylum_"synergistetes"_in_periodontal_health_and_disease_ L2 - https://journals.asm.org/doi/10.1128/AEM.02763-08?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -