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Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization.
Matrix Biol. 2009 May; 28(4):221-9.MB

Abstract

Dentin sialophosphoprotein (DSPP), a major non-collagenous matrix protein of odontoblasts, is proteolytically cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Our previous studies revealed that DSPP null mice display a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposure. Earlier in vitro studies suggested that DPP, but not DSP, plays a significant role in initiation and maturation of dentin mineralization. However, the precise in vivo roles of DSP and DPP are far from clear. Here we report the generation of DPPcKO mice, in which only DSP is expressed in a DSPP null background, resulting in a conditional DPP knockout. DPPcKO teeth show a partial rescue of the DSPP null phenotype with the restored predentin width, an absence of irregular unmineralized areas in dentin, and less frequent pulp exposure. Micro-computed tomography (micro-CT) analysis of DPPcKO molars further confirmed this partial rescue with a significant recovery in the dentin volume, but not in the dentin mineral density. These results indicate distinct roles of DSP and DPP in dentin mineralization, with DSP regulating initiation of dentin mineralization, and DPP being involved in the maturation of mineralized dentin.

Authors+Show Affiliations

Functional Genomics Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health/DHHS, 30 Convent Drive, Bethesda, MD 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

19348940

Citation

Suzuki, Shigeki, et al. "Dentin Sialoprotein and Dentin Phosphoprotein Have Distinct Roles in Dentin Mineralization." Matrix Biology : Journal of the International Society for Matrix Biology, vol. 28, no. 4, 2009, pp. 221-9.
Suzuki S, Sreenath T, Haruyama N, et al. Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. Matrix Biol. 2009;28(4):221-9.
Suzuki, S., Sreenath, T., Haruyama, N., Honeycutt, C., Terse, A., Cho, A., Kohler, T., Müller, R., Goldberg, M., & Kulkarni, A. B. (2009). Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. Matrix Biology : Journal of the International Society for Matrix Biology, 28(4), 221-9. https://doi.org/10.1016/j.matbio.2009.03.006
Suzuki S, et al. Dentin Sialoprotein and Dentin Phosphoprotein Have Distinct Roles in Dentin Mineralization. Matrix Biol. 2009;28(4):221-9. PubMed PMID: 19348940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. AU - Suzuki,Shigeki, AU - Sreenath,Taduru, AU - Haruyama,Naoto, AU - Honeycutt,Cherlita, AU - Terse,Anita, AU - Cho,Andrew, AU - Kohler,Thomas, AU - Müller,Ralph, AU - Goldberg,Michel, AU - Kulkarni,Ashok B, Y1 - 2009/04/05/ PY - 2009/01/13/received PY - 2009/03/17/revised PY - 2009/03/25/accepted PY - 2009/4/8/entrez PY - 2009/4/8/pubmed PY - 2009/9/29/medline SP - 221 EP - 9 JF - Matrix biology : journal of the International Society for Matrix Biology JO - Matrix Biol VL - 28 IS - 4 N2 - Dentin sialophosphoprotein (DSPP), a major non-collagenous matrix protein of odontoblasts, is proteolytically cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Our previous studies revealed that DSPP null mice display a phenotype similar to human autosomal dominant dentinogenesis imperfecta, in which teeth have widened predentin and irregular dentin mineralization resulting in sporadic unmineralized areas in dentin and frequent pulp exposure. Earlier in vitro studies suggested that DPP, but not DSP, plays a significant role in initiation and maturation of dentin mineralization. However, the precise in vivo roles of DSP and DPP are far from clear. Here we report the generation of DPPcKO mice, in which only DSP is expressed in a DSPP null background, resulting in a conditional DPP knockout. DPPcKO teeth show a partial rescue of the DSPP null phenotype with the restored predentin width, an absence of irregular unmineralized areas in dentin, and less frequent pulp exposure. Micro-computed tomography (micro-CT) analysis of DPPcKO molars further confirmed this partial rescue with a significant recovery in the dentin volume, but not in the dentin mineral density. These results indicate distinct roles of DSP and DPP in dentin mineralization, with DSP regulating initiation of dentin mineralization, and DPP being involved in the maturation of mineralized dentin. SN - 1569-1802 UR - https://www.unboundmedicine.com/medline/citation/19348940/Dentin_sialoprotein_and_dentin_phosphoprotein_have_distinct_roles_in_dentin_mineralization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0945-053X(09)00033-X DB - PRIME DP - Unbound Medicine ER -