Tags

Type your tag names separated by a space and hit enter

Enzymatic detoxification of gluten by germinating wheat proteases: implications for new treatment of celiac disease.
Ann Med. 2009; 41(5):390-400.AM

Abstract

INTRODUCTION

Currently the only treatment for celiac disease is a lifelong gluten-free diet. The diet is, however, often burdensome, and thus new treatment options are warranted. We isolated proteases from germinating wheat grain naturally meant for total digestion of wheat storage proteins and investigated whether these enzymes can diminish toxic effects of gluten in vitro and ex vivo.

METHODS

Pepsin and trypsin digested (PT) gliadin was pretreated with proteases from germinating wheat, whereafter the degradation was analyzed by HPLC-MS (high-performance liquid chromatography and mass spectroscopy) and sodium dodecyl sulphate polyacrylamide gel electrophoresis. The toxicity of cleaved PT-gliadin products was assessed in Caco-2 epithelial cells, celiac patient-derived T cells, and in human small intestinal mucosal organ culture biopsies.

RESULTS

Proteases from germinating wheat degraded gliadin into small peptide fragments, which, unlike unprocessed PT-gliadin, did not increase epithelial permeability, induce cytoskeletal rearrangement or changes in ZO-1 expression in Caco-2 cells. Pretreated gliadin did not stimulate T cell proliferation in vitro or enhance the production of autoantibodies to culture supernatants and the activation of CD25+ lymphocytes in the organ culture to the same extent as unprocessed PT-gliadin.

DISCUSSION

Germinating wheat enzymes reduce the toxicity of wheat gliadin in vitro and ex vivo. Further studies are justified to develop an alternative therapy for celiac disease.

Authors+Show Affiliations

Pediatric Research Center, Medical School, University of Tampere, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19353359

Citation

Stenman, Satumarja M., et al. "Enzymatic Detoxification of Gluten By Germinating Wheat Proteases: Implications for New Treatment of Celiac Disease." Annals of Medicine, vol. 41, no. 5, 2009, pp. 390-400.
Stenman SM, Venäläinen JI, Lindfors K, et al. Enzymatic detoxification of gluten by germinating wheat proteases: implications for new treatment of celiac disease. Ann Med. 2009;41(5):390-400.
Stenman, S. M., Venäläinen, J. I., Lindfors, K., Auriola, S., Mauriala, T., Kaukovirta-Norja, A., Jantunen, A., Laurila, K., Qiao, S. W., Sollid, L. M., Männisto, P. T., Kaukinen, K., & Mäki, M. (2009). Enzymatic detoxification of gluten by germinating wheat proteases: implications for new treatment of celiac disease. Annals of Medicine, 41(5), 390-400. https://doi.org/10.1080/07853890902878138
Stenman SM, et al. Enzymatic Detoxification of Gluten By Germinating Wheat Proteases: Implications for New Treatment of Celiac Disease. Ann Med. 2009;41(5):390-400. PubMed PMID: 19353359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzymatic detoxification of gluten by germinating wheat proteases: implications for new treatment of celiac disease. AU - Stenman,Satumarja M, AU - Venäläinen,Jarkko I, AU - Lindfors,Katri, AU - Auriola,Seppo, AU - Mauriala,Timo, AU - Kaukovirta-Norja,Anu, AU - Jantunen,Anna, AU - Laurila,Kaija, AU - Qiao,Shuo-Wang, AU - Sollid,Ludvig M, AU - Männisto,Pekka T, AU - Kaukinen,Katri, AU - Mäki,Markku, PY - 2009/4/9/entrez PY - 2009/4/9/pubmed PY - 2009/6/19/medline SP - 390 EP - 400 JF - Annals of medicine JO - Ann Med VL - 41 IS - 5 N2 - INTRODUCTION: Currently the only treatment for celiac disease is a lifelong gluten-free diet. The diet is, however, often burdensome, and thus new treatment options are warranted. We isolated proteases from germinating wheat grain naturally meant for total digestion of wheat storage proteins and investigated whether these enzymes can diminish toxic effects of gluten in vitro and ex vivo. METHODS: Pepsin and trypsin digested (PT) gliadin was pretreated with proteases from germinating wheat, whereafter the degradation was analyzed by HPLC-MS (high-performance liquid chromatography and mass spectroscopy) and sodium dodecyl sulphate polyacrylamide gel electrophoresis. The toxicity of cleaved PT-gliadin products was assessed in Caco-2 epithelial cells, celiac patient-derived T cells, and in human small intestinal mucosal organ culture biopsies. RESULTS: Proteases from germinating wheat degraded gliadin into small peptide fragments, which, unlike unprocessed PT-gliadin, did not increase epithelial permeability, induce cytoskeletal rearrangement or changes in ZO-1 expression in Caco-2 cells. Pretreated gliadin did not stimulate T cell proliferation in vitro or enhance the production of autoantibodies to culture supernatants and the activation of CD25+ lymphocytes in the organ culture to the same extent as unprocessed PT-gliadin. DISCUSSION: Germinating wheat enzymes reduce the toxicity of wheat gliadin in vitro and ex vivo. Further studies are justified to develop an alternative therapy for celiac disease. SN - 1365-2060 UR - https://www.unboundmedicine.com/medline/citation/19353359/Enzymatic_detoxification_of_gluten_by_germinating_wheat_proteases:_implications_for_new_treatment_of_celiac_disease_ L2 - https://www.tandfonline.com/doi/full/10.1080/07853890902878138 DB - PRIME DP - Unbound Medicine ER -