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Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples.
Res Microbiol. 2009 Apr; 160(3):193-9.RM

Abstract

Three Bacillus strains isolated from honey samples and bee gut were pre-selected for their in vitro antimicrobial activity against Paenibacillus larvae and Ascosphaera apis, important honeybee pathogens. The analysis of their 16S rRNA sequences revealed that C4, M1 and G2III strains belong to the subtilis species. Surfactin synthesis was verified by IR spectroscopy and HPLC studies. Surfactin inhibited P. larvae but it failed to affect A. apis. Vegetative cells of P. larvae were affected as soon as they came in contact with the surfactin sample; two orders of magnitude less in log scale were recorded. Optimal surfactin production was observed in MEL medium, a broth with molasses as the only carbon source. Bacillus subtilis G2III strain exhibited the highest levels of surfactin synthesis in BHI and MEL broths: 1391AU/ml and 2782AU/ml, respectively. Since only A. apis inhibition was observed when cell suspensions were assayed, we suspect that there may be an antimycotic compound within cells. The co-production of surfactin and a fungicide by these strains might biologically control bee pathogens in apiculture.

Authors+Show Affiliations

Instituto de Investigaciones para la Industria Química (INIQUI), A4402 FDC-Salta, Salta, Argentina.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19358885

Citation

Sabaté, Daniela C., et al. "Inhibition of Paenibacillus Larvae and Ascosphaera Apis By Bacillus Subtilis Isolated From Honeybee Gut and Honey Samples." Research in Microbiology, vol. 160, no. 3, 2009, pp. 193-9.
Sabaté DC, Carrillo L, Audisio MC. Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples. Res Microbiol. 2009;160(3):193-9.
Sabaté, D. C., Carrillo, L., & Audisio, M. C. (2009). Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples. Research in Microbiology, 160(3), 193-9. https://doi.org/10.1016/j.resmic.2009.03.002
Sabaté DC, Carrillo L, Audisio MC. Inhibition of Paenibacillus Larvae and Ascosphaera Apis By Bacillus Subtilis Isolated From Honeybee Gut and Honey Samples. Res Microbiol. 2009;160(3):193-9. PubMed PMID: 19358885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples. AU - Sabaté,Daniela C, AU - Carrillo,Leonor, AU - Audisio,M Carina, Y1 - 2009/04/07/ PY - 2008/12/31/received PY - 2009/03/05/revised PY - 2009/03/06/accepted PY - 2009/4/11/entrez PY - 2009/4/11/pubmed PY - 2009/8/4/medline SP - 193 EP - 9 JF - Research in microbiology JO - Res Microbiol VL - 160 IS - 3 N2 - Three Bacillus strains isolated from honey samples and bee gut were pre-selected for their in vitro antimicrobial activity against Paenibacillus larvae and Ascosphaera apis, important honeybee pathogens. The analysis of their 16S rRNA sequences revealed that C4, M1 and G2III strains belong to the subtilis species. Surfactin synthesis was verified by IR spectroscopy and HPLC studies. Surfactin inhibited P. larvae but it failed to affect A. apis. Vegetative cells of P. larvae were affected as soon as they came in contact with the surfactin sample; two orders of magnitude less in log scale were recorded. Optimal surfactin production was observed in MEL medium, a broth with molasses as the only carbon source. Bacillus subtilis G2III strain exhibited the highest levels of surfactin synthesis in BHI and MEL broths: 1391AU/ml and 2782AU/ml, respectively. Since only A. apis inhibition was observed when cell suspensions were assayed, we suspect that there may be an antimycotic compound within cells. The co-production of surfactin and a fungicide by these strains might biologically control bee pathogens in apiculture. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/19358885/Inhibition_of_Paenibacillus_larvae_and_Ascosphaera_apis_by_Bacillus_subtilis_isolated_from_honeybee_gut_and_honey_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(09)00024-2 DB - PRIME DP - Unbound Medicine ER -