Tags

Type your tag names separated by a space and hit enter

Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography.
J Sep Sci. 2009 May; 32(9):1284-93.JS

Abstract

An environment-friendly method was established for the preparative separation and enrichment of four taxoids, namely 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), cephalomannine and paclitaxel from Taxus chinensis needles extracts. Characteristics of seven widely used macroporous resins for four taxoids were compared, AB-8 resin offered better adsorption and desorption capacities than others. AB-8 resin column chromatography was used to study the desorption process for four taxoids. The optimum parameters for desorption were 30% ethanol 5 RV for removing impurities, following 15 RV for 10-DAB III, after the desorption of impurities with 35% ethanol 10 RV, 45% ethanol 30 RV for 7-xyl-10-DAT, then 65% ethanol 10 RV for cephalomannine and paclitaxel, the flow rate was 6 RV/h. After separation on AB-8 resin column chromatography, the contents of 10-DAB III, 7-xyl-10-DAT, cephalomannine and paclitaxel in the product reached 4.58, 13.17, 1.36 and 3.08%, respectively, which were 7.63-, 3.68-, 6.18- and 6.55-fold to those in T. chinensis needles extracts. The recovery yields were 94.96, 77.32, 88.09 and 95.25%. In general, the AB-8 resin column chromatography has the advantages of lower cost, high efficiency and simple procedure. Therefore, it may provide scientific references for the preparative separation and enrichment of taxoids from other T. species.

Authors+Show Affiliations

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19360728

Citation

Sun, Rui, et al. "Preparative Separation and Enrichment of Four Taxoids From Taxus Chinensis Needles Extracts By Macroporous Resin Column Chromatography." Journal of Separation Science, vol. 32, no. 9, 2009, pp. 1284-93.
Sun R, Fu K, Fu Y, et al. Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography. J Sep Sci. 2009;32(9):1284-93.
Sun, R., Fu, K., Fu, Y., Zu, Y., Wang, Y., Luo, M., Li, S., Luo, H., & Li, Z. (2009). Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography. Journal of Separation Science, 32(9), 1284-93. https://doi.org/10.1002/jssc.200800689
Sun R, et al. Preparative Separation and Enrichment of Four Taxoids From Taxus Chinensis Needles Extracts By Macroporous Resin Column Chromatography. J Sep Sci. 2009;32(9):1284-93. PubMed PMID: 19360728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography. AU - Sun,Rui, AU - Fu,Kusng, AU - Fu,Yujie, AU - Zu,Yuangang, AU - Wang,Ying, AU - Luo,Meng, AU - Li,Shuangming, AU - Luo,Hao, AU - Li,Zhengnan, PY - 2009/4/11/entrez PY - 2009/4/11/pubmed PY - 2009/7/17/medline SP - 1284 EP - 93 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 9 N2 - An environment-friendly method was established for the preparative separation and enrichment of four taxoids, namely 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), cephalomannine and paclitaxel from Taxus chinensis needles extracts. Characteristics of seven widely used macroporous resins for four taxoids were compared, AB-8 resin offered better adsorption and desorption capacities than others. AB-8 resin column chromatography was used to study the desorption process for four taxoids. The optimum parameters for desorption were 30% ethanol 5 RV for removing impurities, following 15 RV for 10-DAB III, after the desorption of impurities with 35% ethanol 10 RV, 45% ethanol 30 RV for 7-xyl-10-DAT, then 65% ethanol 10 RV for cephalomannine and paclitaxel, the flow rate was 6 RV/h. After separation on AB-8 resin column chromatography, the contents of 10-DAB III, 7-xyl-10-DAT, cephalomannine and paclitaxel in the product reached 4.58, 13.17, 1.36 and 3.08%, respectively, which were 7.63-, 3.68-, 6.18- and 6.55-fold to those in T. chinensis needles extracts. The recovery yields were 94.96, 77.32, 88.09 and 95.25%. In general, the AB-8 resin column chromatography has the advantages of lower cost, high efficiency and simple procedure. Therefore, it may provide scientific references for the preparative separation and enrichment of taxoids from other T. species. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19360728/Preparative_separation_and_enrichment_of_four_taxoids_from_Taxus_chinensis_needles_extracts_by_macroporous_resin_column_chromatography_ L2 - https://doi.org/10.1002/jssc.200800689 DB - PRIME DP - Unbound Medicine ER -