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Photoconversion in orange and red fluorescent proteins.
Nat Methods 2009; 6(5):355-8NM

Abstract

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.

Authors+Show Affiliations

Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19363494

Citation

Kremers, Gert-Jan, et al. "Photoconversion in Orange and Red Fluorescent Proteins." Nature Methods, vol. 6, no. 5, 2009, pp. 355-8.
Kremers GJ, Hazelwood KL, Murphy CS, et al. Photoconversion in orange and red fluorescent proteins. Nat Methods. 2009;6(5):355-8.
Kremers, G. J., Hazelwood, K. L., Murphy, C. S., Davidson, M. W., & Piston, D. W. (2009). Photoconversion in orange and red fluorescent proteins. Nature Methods, 6(5), pp. 355-8. doi:10.1038/nmeth.1319.
Kremers GJ, et al. Photoconversion in Orange and Red Fluorescent Proteins. Nat Methods. 2009;6(5):355-8. PubMed PMID: 19363494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photoconversion in orange and red fluorescent proteins. AU - Kremers,Gert-Jan, AU - Hazelwood,Kristin L, AU - Murphy,Christopher S, AU - Davidson,Michael W, AU - Piston,David W, Y1 - 2009/04/12/ PY - 2008/12/24/received PY - 2009/03/12/accepted PY - 2009/4/14/entrez PY - 2009/4/14/pubmed PY - 2009/5/27/medline SP - 355 EP - 8 JF - Nature methods JO - Nat. Methods VL - 6 IS - 5 N2 - We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date. SN - 1548-7105 UR - https://www.unboundmedicine.com/medline/citation/19363494/Photoconversion_in_orange_and_red_fluorescent_proteins_ L2 - http://dx.doi.org/10.1038/nmeth.1319 DB - PRIME DP - Unbound Medicine ER -