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Phylogenetic assessment of the earthworm Aporrectodea caliginosa species complex (Oligochaeta: Lumbricidae) based on mitochondrial and nuclear DNA sequences.
Mol Phylogenet Evol. 2009 Aug; 52(2):293-302.MP

Abstract

The Aporrectodea caliginosa species complex includes the most abundant earthworms in grasslands and agricultural ecosystems of the Paleartic region. Historically this complex consisted of the following taxa: A. caliginosa s.s.Savigny, 1826, A. trapezoides Dugés (1828), A. tuberculata (Eisen, 1874), and A. nocturna Evans (1946). These four taxa are morphologically very similar and difficult to differentiate because of their morphological variability. Consequently, their taxonomic status and their phylogenetic relationships have been a matter of discussion for more than a century. To study these questions, we sequenced the COII (686 bp), 12S (362 bp), 16S (1200 bp), ND1 (917 bp), and tRNAs(Asn-Asp-Val-Leu-Ala-Ser-Leu) (402 bp) mitochondrial and 28S (809 bp) nuclear gene regions for 85 European earthworms from 27 different localities belonging to the A. caliginosa species complex and four outgroup taxa. DNA sequences were analyzed using maximum parsimony, maximum likelihood, and Bayesian approaches of phylogenetic inference. The resulting trees were combined with morphological, ecological, and genomic evidence to test species boundaries (i.e., integrative approach). Our molecular analyses showed that A. caliginosa s.s. and A. tuberculata form a sister clade to A. trapezoides, A. longa, and A. nocturna, which indicates that A. longa is part of the A. caliginosa species complex. We confirm the species status of all these taxa and identify two hitherto unrecognized Aporrectodea species in Corsica (France). Moreover our analyses also showed the presence of highly divergent lineages within A. caliginosa, A. trapezoides, and A. longa, suggesting the existence of cryptic diversity within these taxa.

Authors+Show Affiliations

CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal. mlosada@genoma-llc.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19364539

Citation

Pérez-Losada, Marcos, et al. "Phylogenetic Assessment of the Earthworm Aporrectodea Caliginosa Species Complex (Oligochaeta: Lumbricidae) Based On Mitochondrial and Nuclear DNA Sequences." Molecular Phylogenetics and Evolution, vol. 52, no. 2, 2009, pp. 293-302.
Pérez-Losada M, Ricoy M, Marshall JC, et al. Phylogenetic assessment of the earthworm Aporrectodea caliginosa species complex (Oligochaeta: Lumbricidae) based on mitochondrial and nuclear DNA sequences. Mol Phylogenet Evol. 2009;52(2):293-302.
Pérez-Losada, M., Ricoy, M., Marshall, J. C., & Domínguez, J. (2009). Phylogenetic assessment of the earthworm Aporrectodea caliginosa species complex (Oligochaeta: Lumbricidae) based on mitochondrial and nuclear DNA sequences. Molecular Phylogenetics and Evolution, 52(2), 293-302. https://doi.org/10.1016/j.ympev.2009.04.003
Pérez-Losada M, et al. Phylogenetic Assessment of the Earthworm Aporrectodea Caliginosa Species Complex (Oligochaeta: Lumbricidae) Based On Mitochondrial and Nuclear DNA Sequences. Mol Phylogenet Evol. 2009;52(2):293-302. PubMed PMID: 19364539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phylogenetic assessment of the earthworm Aporrectodea caliginosa species complex (Oligochaeta: Lumbricidae) based on mitochondrial and nuclear DNA sequences. AU - Pérez-Losada,Marcos, AU - Ricoy,Maigualida, AU - Marshall,Jonathon C, AU - Domínguez,Jorge, Y1 - 2009/04/11/ PY - 2008/09/18/received PY - 2009/01/23/revised PY - 2009/04/03/accepted PY - 2009/4/15/entrez PY - 2009/4/15/pubmed PY - 2009/6/16/medline SP - 293 EP - 302 JF - Molecular phylogenetics and evolution JO - Mol Phylogenet Evol VL - 52 IS - 2 N2 - The Aporrectodea caliginosa species complex includes the most abundant earthworms in grasslands and agricultural ecosystems of the Paleartic region. Historically this complex consisted of the following taxa: A. caliginosa s.s.Savigny, 1826, A. trapezoides Dugés (1828), A. tuberculata (Eisen, 1874), and A. nocturna Evans (1946). These four taxa are morphologically very similar and difficult to differentiate because of their morphological variability. Consequently, their taxonomic status and their phylogenetic relationships have been a matter of discussion for more than a century. To study these questions, we sequenced the COII (686 bp), 12S (362 bp), 16S (1200 bp), ND1 (917 bp), and tRNAs(Asn-Asp-Val-Leu-Ala-Ser-Leu) (402 bp) mitochondrial and 28S (809 bp) nuclear gene regions for 85 European earthworms from 27 different localities belonging to the A. caliginosa species complex and four outgroup taxa. DNA sequences were analyzed using maximum parsimony, maximum likelihood, and Bayesian approaches of phylogenetic inference. The resulting trees were combined with morphological, ecological, and genomic evidence to test species boundaries (i.e., integrative approach). Our molecular analyses showed that A. caliginosa s.s. and A. tuberculata form a sister clade to A. trapezoides, A. longa, and A. nocturna, which indicates that A. longa is part of the A. caliginosa species complex. We confirm the species status of all these taxa and identify two hitherto unrecognized Aporrectodea species in Corsica (France). Moreover our analyses also showed the presence of highly divergent lineages within A. caliginosa, A. trapezoides, and A. longa, suggesting the existence of cryptic diversity within these taxa. SN - 1095-9513 UR - https://www.unboundmedicine.com/medline/citation/19364539/Phylogenetic_assessment_of_the_earthworm_Aporrectodea_caliginosa_species_complex__Oligochaeta:_Lumbricidae__based_on_mitochondrial_and_nuclear_DNA_sequences_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1055-7903(09)00128-6 DB - PRIME DP - Unbound Medicine ER -