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Comparison of renal dysfunction in wild-type, IL-6 KO and iNOS KO mice hind limb tourniquet-reperfusion model.
Leg Med (Tokyo). 2009 Apr; 11 Suppl 1:S248-51.LM

Abstract

The release of a tourniquet after hind limb ischemia results in vital organ injury, which progresses to multiple organ failure with a high mortality rate. Many events are involved in ischemia-reperfusion (I/R) injury. The purpose of this study was to determine how IL-6 or iNOS is involved in I/R injury using IL-6 knockout (KO) and iNOS KO mice. Male C57BL/6J wild-type (WT), IL-6 knockout (KO) and iNOS KO mice were anesthetized with pentobarbital, and rubber bands were fastened to the inguinal region of both hind limbs for 3h. Blood and kidney samples were obtained before reperfusion and at 1, 2, 3, and 12h after reperfusion. For the control group, mice were kept for 6h under an anesthetized condition without rubber bands. Blood gases and biochemical parameters were analyzed by i-STAT300F. Real-time PCR analyses were performed to examine the expression levels of IL-6 and iNOS mRNA in kidneys. Metabolic acidosis, hemoconcentration and renal dysfunction were significantly developed after reperfusion regardless of mouse genotype, and progression of this condition was earlier in IL-6 KO and iNOS KO mice than in WT mice. The expression level of kidney IL-6 mRNA increased and that of iNOS mRNA decreased after reperfusion. It is possible that late decrease recovery of iNOS mRNA expression in IL-6 KO mice and early progress of IL-6 mRNA expression in iNOS KO mice after reperfusion induce renal dysfunction.

Authors+Show Affiliations

Department of Legal Medicine, Fukushima Medical University School of Medicine, Hikarigaoka 1, Fukushima 960-1295, Japan. cato@fmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19364673

Citation

Kato, Naho, et al. "Comparison of Renal Dysfunction in Wild-type, IL-6 KO and iNOS KO Mice Hind Limb Tourniquet-reperfusion Model." Legal Medicine (Tokyo, Japan), vol. 11 Suppl 1, 2009, pp. S248-51.
Kato N, Abe S, Suto M, et al. Comparison of renal dysfunction in wild-type, IL-6 KO and iNOS KO mice hind limb tourniquet-reperfusion model. Leg Med (Tokyo). 2009;11 Suppl 1:S248-51.
Kato, N., Abe, S., Suto, M., & Hiraiwa, K. (2009). Comparison of renal dysfunction in wild-type, IL-6 KO and iNOS KO mice hind limb tourniquet-reperfusion model. Legal Medicine (Tokyo, Japan), 11 Suppl 1, S248-51. https://doi.org/10.1016/j.legalmed.2009.03.006
Kato N, et al. Comparison of Renal Dysfunction in Wild-type, IL-6 KO and iNOS KO Mice Hind Limb Tourniquet-reperfusion Model. Leg Med (Tokyo). 2009;11 Suppl 1:S248-51. PubMed PMID: 19364673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of renal dysfunction in wild-type, IL-6 KO and iNOS KO mice hind limb tourniquet-reperfusion model. AU - Kato,Naho, AU - Abe,Sumiko, AU - Suto,Miwako, AU - Hiraiwa,Kouichi, Y1 - 2009/04/11/ PY - 2008/12/16/received PY - 2009/02/24/revised PY - 2009/03/05/accepted PY - 2009/4/15/entrez PY - 2009/4/15/pubmed PY - 2010/1/27/medline SP - S248 EP - 51 JF - Legal medicine (Tokyo, Japan) JO - Leg Med (Tokyo) VL - 11 Suppl 1 N2 - The release of a tourniquet after hind limb ischemia results in vital organ injury, which progresses to multiple organ failure with a high mortality rate. Many events are involved in ischemia-reperfusion (I/R) injury. The purpose of this study was to determine how IL-6 or iNOS is involved in I/R injury using IL-6 knockout (KO) and iNOS KO mice. Male C57BL/6J wild-type (WT), IL-6 knockout (KO) and iNOS KO mice were anesthetized with pentobarbital, and rubber bands were fastened to the inguinal region of both hind limbs for 3h. Blood and kidney samples were obtained before reperfusion and at 1, 2, 3, and 12h after reperfusion. For the control group, mice were kept for 6h under an anesthetized condition without rubber bands. Blood gases and biochemical parameters were analyzed by i-STAT300F. Real-time PCR analyses were performed to examine the expression levels of IL-6 and iNOS mRNA in kidneys. Metabolic acidosis, hemoconcentration and renal dysfunction were significantly developed after reperfusion regardless of mouse genotype, and progression of this condition was earlier in IL-6 KO and iNOS KO mice than in WT mice. The expression level of kidney IL-6 mRNA increased and that of iNOS mRNA decreased after reperfusion. It is possible that late decrease recovery of iNOS mRNA expression in IL-6 KO mice and early progress of IL-6 mRNA expression in iNOS KO mice after reperfusion induce renal dysfunction. SN - 1873-4162 UR - https://www.unboundmedicine.com/medline/citation/19364673/Comparison_of_renal_dysfunction_in_wild_type_IL_6_KO_and_iNOS_KO_mice_hind_limb_tourniquet_reperfusion_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1344-6223(09)00222-3 DB - PRIME DP - Unbound Medicine ER -