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DNA microarray-based detection and identification of Burkholderia mallei, Burkholderia pseudomallei and Burkholderia spp.
Mol Cell Probes. 2009 Jun-Aug; 23(3-4):178-87.MC

Abstract

We developed a rapid oligonucleotide microarray assay based on genetic markers for the accurate identification and differentiation of Burkholderia (B.) mallei and Burkholderia pseudomallei, the agents of glanders and melioidosis, respectively. These two agents were clearly identified using at least 4 independent genetic markers including 16S rRNA gene, fliC, motB and also by novel species-specific target genes, identified by in silico sequence analysis. Specific hybridization signal profiles allowed the detection and differentiation of up to 10 further Burkholderia spp., including the closely related species Burkholderia thailandensis and Burkholderia-like agents, such as Burkholderia cepacia, Burkholderia cenocepacia, Burkholderia vietnamiensis, Burkholderia ambifaria, and Burkholderia gladioli, which are often associated with cystic fibrosis (CF) lung disease. The assay was developed using the easy-to-handle and economical ArrayTube (AT) platform. A representative strain panel comprising 44 B. mallei, 32 B. pseudomallei isolates, and various Burkholderia type strains were examined to validate the test. Assay specificity was determined by examination of 40 non-Burkholderia strains.

Authors+Show Affiliations

Institute of Bacterial Infections and Zoonoses at the Federal Research Institute for Animal Health (Friedrich-Loeffler-Institut), Naumburger Strasse 96a, 07743 Jena, Germany. gernot.schmoock@fli.bund.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19366627

Citation

Schmoock, Gernot, et al. "DNA Microarray-based Detection and Identification of Burkholderia Mallei, Burkholderia Pseudomallei and Burkholderia Spp." Molecular and Cellular Probes, vol. 23, no. 3-4, 2009, pp. 178-87.
Schmoock G, Ehricht R, Melzer F, et al. DNA microarray-based detection and identification of Burkholderia mallei, Burkholderia pseudomallei and Burkholderia spp. Mol Cell Probes. 2009;23(3-4):178-87.
Schmoock, G., Ehricht, R., Melzer, F., Rassbach, A., Scholz, H. C., Neubauer, H., Sachse, K., Mota, R. A., Saqib, M., & Elschner, M. (2009). DNA microarray-based detection and identification of Burkholderia mallei, Burkholderia pseudomallei and Burkholderia spp. Molecular and Cellular Probes, 23(3-4), 178-87. https://doi.org/10.1016/j.mcp.2009.04.001
Schmoock G, et al. DNA Microarray-based Detection and Identification of Burkholderia Mallei, Burkholderia Pseudomallei and Burkholderia Spp. Mol Cell Probes. 2009 Jun-Aug;23(3-4):178-87. PubMed PMID: 19366627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA microarray-based detection and identification of Burkholderia mallei, Burkholderia pseudomallei and Burkholderia spp. AU - Schmoock,Gernot, AU - Ehricht,Ralf, AU - Melzer,Falk, AU - Rassbach,Astrid, AU - Scholz,Holger C, AU - Neubauer,Heinrich, AU - Sachse,Konrad, AU - Mota,Rinaldo Aparecido, AU - Saqib,Muhammad, AU - Elschner,Mandy, Y1 - 2009/04/12/ PY - 2008/12/16/received PY - 2009/04/03/revised PY - 2009/04/06/accepted PY - 2009/4/16/entrez PY - 2009/4/16/pubmed PY - 2009/8/21/medline SP - 178 EP - 87 JF - Molecular and cellular probes JO - Mol Cell Probes VL - 23 IS - 3-4 N2 - We developed a rapid oligonucleotide microarray assay based on genetic markers for the accurate identification and differentiation of Burkholderia (B.) mallei and Burkholderia pseudomallei, the agents of glanders and melioidosis, respectively. These two agents were clearly identified using at least 4 independent genetic markers including 16S rRNA gene, fliC, motB and also by novel species-specific target genes, identified by in silico sequence analysis. Specific hybridization signal profiles allowed the detection and differentiation of up to 10 further Burkholderia spp., including the closely related species Burkholderia thailandensis and Burkholderia-like agents, such as Burkholderia cepacia, Burkholderia cenocepacia, Burkholderia vietnamiensis, Burkholderia ambifaria, and Burkholderia gladioli, which are often associated with cystic fibrosis (CF) lung disease. The assay was developed using the easy-to-handle and economical ArrayTube (AT) platform. A representative strain panel comprising 44 B. mallei, 32 B. pseudomallei isolates, and various Burkholderia type strains were examined to validate the test. Assay specificity was determined by examination of 40 non-Burkholderia strains. SN - 1096-1194 UR - https://www.unboundmedicine.com/medline/citation/19366627/DNA_microarray_based_detection_and_identification_of_Burkholderia_mallei_Burkholderia_pseudomallei_and_Burkholderia_spp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-8508(09)00029-2 DB - PRIME DP - Unbound Medicine ER -