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Quantitative prediction of cell wall polysaccharide composition in grape (Vitis vinifera L.) and apple (Malus domestica) skins from acid hydrolysis monosaccharide profiles.
J Agric Food Chem. 2009 May 13; 57(9):3611-9.JA

Abstract

On the basis of monosaccharide analysis after acid hydrolysis of fruit skin samples of three wine grape cultivars, Vitis vinifera L. Cabernet Sauvignon, Merlot, and Shiraz, and of two types of apple, Malus domestica Red Delicious and Golden Delicious, an iterative calculation method is reported for the quantitative allocation of plant cell wall monomers into relevant structural polysaccharide elements. By this method the relative molar distribution (mol %) of the different polysaccharides in the red wine grape skins was estimated as 57-62 mol % homogalacturonan, 6.0-14 mol % cellulose, 10-11 mol % xyloglucan, 7 mol % arabinan, 4.5-5.0 mol % rhamnogalacturonan I, 3.5-4.0 mol % rhamnogalacturonan II, 3 mol % arabinogalactan, and 0.5-1.0 mol % mannans; the ranges indicate minor variations in the skin composition of the three different cultivars. These cell wall polysaccharides made up approximately 43-47% by weight of the skins (dry matter), the rest mainly being lignin. The predicted relative molar levels of the polysaccharide elements in the apple skins, which made up approximately 49-64% by weight of the skins (dry matter), appeared to be similar to those of the grape skins. The apple skins were estimated to be relatively richer than grape skins in arabinan, total levels 10-13 mol %, and relatively lower in mannan content, total levels </=0.3 mol %. The data also demonstrate the superiority of trifluoroacetic acid to hydrochloric acid for hydrolysis of plant cell wall material to monosaccharides, notably with respect to the galacturonic acid levels and, in turn, in relation to predicting the relative contents of structural pectin elements in the plant cell wall substrates.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Arnous A
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Meyer AS
No affiliation info available

MeSH

Cell WallFruitHexuronic AcidsHydrochloric AcidHydrogen-Ion ConcentrationHydrolysisMalusMonosaccharidesPolysaccharidesTrifluoroacetic AcidVitis

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19371033

Citation

Arnous, Anis, and Anne S. Meyer. "Quantitative Prediction of Cell Wall Polysaccharide Composition in Grape (Vitis Vinifera L.) and Apple (Malus Domestica) Skins From Acid Hydrolysis Monosaccharide Profiles." Journal of Agricultural and Food Chemistry, vol. 57, no. 9, 2009, pp. 3611-9.
Arnous A, Meyer AS. Quantitative prediction of cell wall polysaccharide composition in grape (Vitis vinifera L.) and apple (Malus domestica) skins from acid hydrolysis monosaccharide profiles. J Agric Food Chem. 2009;57(9):3611-9.
Arnous, A., & Meyer, A. S. (2009). Quantitative prediction of cell wall polysaccharide composition in grape (Vitis vinifera L.) and apple (Malus domestica) skins from acid hydrolysis monosaccharide profiles. Journal of Agricultural and Food Chemistry, 57(9), 3611-9. https://doi.org/10.1021/jf900780r
Arnous A, Meyer AS. Quantitative Prediction of Cell Wall Polysaccharide Composition in Grape (Vitis Vinifera L.) and Apple (Malus Domestica) Skins From Acid Hydrolysis Monosaccharide Profiles. J Agric Food Chem. 2009 May 13;57(9):3611-9. PubMed PMID: 19371033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative prediction of cell wall polysaccharide composition in grape (Vitis vinifera L.) and apple (Malus domestica) skins from acid hydrolysis monosaccharide profiles. AU - Arnous,Anis, AU - Meyer,Anne S, PY - 2009/4/18/entrez PY - 2009/4/18/pubmed PY - 2009/7/31/medline SP - 3611 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 9 N2 - On the basis of monosaccharide analysis after acid hydrolysis of fruit skin samples of three wine grape cultivars, Vitis vinifera L. Cabernet Sauvignon, Merlot, and Shiraz, and of two types of apple, Malus domestica Red Delicious and Golden Delicious, an iterative calculation method is reported for the quantitative allocation of plant cell wall monomers into relevant structural polysaccharide elements. By this method the relative molar distribution (mol %) of the different polysaccharides in the red wine grape skins was estimated as 57-62 mol % homogalacturonan, 6.0-14 mol % cellulose, 10-11 mol % xyloglucan, 7 mol % arabinan, 4.5-5.0 mol % rhamnogalacturonan I, 3.5-4.0 mol % rhamnogalacturonan II, 3 mol % arabinogalactan, and 0.5-1.0 mol % mannans; the ranges indicate minor variations in the skin composition of the three different cultivars. These cell wall polysaccharides made up approximately 43-47% by weight of the skins (dry matter), the rest mainly being lignin. The predicted relative molar levels of the polysaccharide elements in the apple skins, which made up approximately 49-64% by weight of the skins (dry matter), appeared to be similar to those of the grape skins. The apple skins were estimated to be relatively richer than grape skins in arabinan, total levels 10-13 mol %, and relatively lower in mannan content, total levels </=0.3 mol %. The data also demonstrate the superiority of trifluoroacetic acid to hydrochloric acid for hydrolysis of plant cell wall material to monosaccharides, notably with respect to the galacturonic acid levels and, in turn, in relation to predicting the relative contents of structural pectin elements in the plant cell wall substrates. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19371033/Quantitative_prediction_of_cell_wall_polysaccharide_composition_in_grape__Vitis_vinifera_L___and_apple__Malus_domestica__skins_from_acid_hydrolysis_monosaccharide_profiles_ L2 - https://doi.org/10.1021/jf900780r DB - PRIME DP - Unbound Medicine ER -