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Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells.
Eur J Pharmacol. 2009 Mar 15; 606(1-3):25-31.EJ

Abstract

Regulator of G protein signalling (RGS) protein expression is altered under growth promoting conditions in vascular smooth muscle cells (VSMCs). Since sphingosine-1-phosphate (S1P) is an important growth stimulatory factor, we investigated whether stimulation of VSMCs with S1P results in alterations in mRNA expression levels of several RGS proteins and which signalling components are involved. VSMCs were stimulated with S1P and mRNA expression levels of RGS2, RGS3, RGS4, RGS5 and RGS16 were measured by real-time polymerase chain reaction. S1P caused a time-dependent up-regulation of RGS2 and RGS16 mRNA expression. FTY720-P, a S1P(1)/S1P(3-5) agonist, did not regulate RGS2 mRNA levels although it did up-regulate RGS16 mRNA expression. Pertussis toxin treatment revealed that the S1P-induced RGS16 expression was G(i/o)-dependent whereas up-regulation of RGS2 mRNA was not. Phosphatidylinositol 3-kinase, protein kinase C and mitogen-activated protein kinase kinase apparently were not involved in the S1P-induced up-regulation of both RGS proteins. The present study demonstrates that S1P induces RGS2 and RGS16 mRNA expression but uses distinct S1P receptor subtypes and signalling pathways to regulate expression of these RGS proteins.

Authors+Show Affiliations

Dept Pharmacology & Pharmacotherapy, Academic Medical Center, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19374869

Citation

Hendriks-Balk, Mariëlle C., et al. "Sphingosine-1-phosphate Regulates RGS2 and RGS16 mRNA Expression in Vascular Smooth Muscle Cells." European Journal of Pharmacology, vol. 606, no. 1-3, 2009, pp. 25-31.
Hendriks-Balk MC, Hajji N, van Loenen PB, et al. Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells. Eur J Pharmacol. 2009;606(1-3):25-31.
Hendriks-Balk, M. C., Hajji, N., van Loenen, P. B., Michel, M. C., Peters, S. L., & Alewijnse, A. E. (2009). Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells. European Journal of Pharmacology, 606(1-3), 25-31. https://doi.org/10.1016/j.ejphar.2009.01.018
Hendriks-Balk MC, et al. Sphingosine-1-phosphate Regulates RGS2 and RGS16 mRNA Expression in Vascular Smooth Muscle Cells. Eur J Pharmacol. 2009 Mar 15;606(1-3):25-31. PubMed PMID: 19374869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells. AU - Hendriks-Balk,Mariëlle C, AU - Hajji,Najat, AU - van Loenen,Pieter B, AU - Michel,Martin C, AU - Peters,Stephan L M, AU - Alewijnse,Astrid E, Y1 - 2009/01/21/ PY - 2008/10/20/received PY - 2008/12/19/revised PY - 2009/01/09/accepted PY - 2009/4/21/entrez PY - 2009/4/21/pubmed PY - 2009/9/2/medline SP - 25 EP - 31 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 606 IS - 1-3 N2 - Regulator of G protein signalling (RGS) protein expression is altered under growth promoting conditions in vascular smooth muscle cells (VSMCs). Since sphingosine-1-phosphate (S1P) is an important growth stimulatory factor, we investigated whether stimulation of VSMCs with S1P results in alterations in mRNA expression levels of several RGS proteins and which signalling components are involved. VSMCs were stimulated with S1P and mRNA expression levels of RGS2, RGS3, RGS4, RGS5 and RGS16 were measured by real-time polymerase chain reaction. S1P caused a time-dependent up-regulation of RGS2 and RGS16 mRNA expression. FTY720-P, a S1P(1)/S1P(3-5) agonist, did not regulate RGS2 mRNA levels although it did up-regulate RGS16 mRNA expression. Pertussis toxin treatment revealed that the S1P-induced RGS16 expression was G(i/o)-dependent whereas up-regulation of RGS2 mRNA was not. Phosphatidylinositol 3-kinase, protein kinase C and mitogen-activated protein kinase kinase apparently were not involved in the S1P-induced up-regulation of both RGS proteins. The present study demonstrates that S1P induces RGS2 and RGS16 mRNA expression but uses distinct S1P receptor subtypes and signalling pathways to regulate expression of these RGS proteins. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/19374869/Sphingosine_1_phosphate_regulates_RGS2_and_RGS16_mRNA_expression_in_vascular_smooth_muscle_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(09)00074-0 DB - PRIME DP - Unbound Medicine ER -