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Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways.
Food Chem Toxicol 2009; 47(7):1610-7FC

Abstract

Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. In this study, we evaluated the anti-inflammatory effects of anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappaB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappaB by blocking the phosphorylation of inhibitory kappa B-alpha (IkappaB-alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway. Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappaB transcriptional activity, possibly via the p38 MAP kinase pathway.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University, Dongdaemun-Ku, Hoegi-Dong, Seoul 130-701, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19375480

Citation

Kim, Ji-Yeon, et al. "Anti-inflammatory Effect of Anemarsaponin B Isolated From the Rhizomes of Anemarrhena Asphodeloides in LPS-induced RAW 264.7 Macrophages Is Mediated By Negative Regulation of the Nuclear factor-kappaB and P38 Pathways." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 47, no. 7, 2009, pp. 1610-7.
Kim JY, Shin JS, Ryu JH, et al. Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways. Food Chem Toxicol. 2009;47(7):1610-7.
Kim, J. Y., Shin, J. S., Ryu, J. H., Kim, S. Y., Cho, Y. W., Choi, J. H., & Lee, K. T. (2009). Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 47(7), pp. 1610-7. doi:10.1016/j.fct.2009.04.009.
Kim JY, et al. Anti-inflammatory Effect of Anemarsaponin B Isolated From the Rhizomes of Anemarrhena Asphodeloides in LPS-induced RAW 264.7 Macrophages Is Mediated By Negative Regulation of the Nuclear factor-kappaB and P38 Pathways. Food Chem Toxicol. 2009;47(7):1610-7. PubMed PMID: 19375480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways. AU - Kim,Ji-Yeon, AU - Shin,Ji-Sun, AU - Ryu,Jong Hoon, AU - Kim,Sun Yeou, AU - Cho,Young-Wuk, AU - Choi,Jung-Hye, AU - Lee,Kyung-Tae, Y1 - 2009/04/16/ PY - 2008/12/31/received PY - 2009/03/31/revised PY - 2009/04/07/accepted PY - 2009/4/21/entrez PY - 2009/4/21/pubmed PY - 2009/8/29/medline SP - 1610 EP - 7 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 47 IS - 7 N2 - Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. In this study, we evaluated the anti-inflammatory effects of anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappaB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappaB by blocking the phosphorylation of inhibitory kappa B-alpha (IkappaB-alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway. Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappaB transcriptional activity, possibly via the p38 MAP kinase pathway. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19375480/Anti_inflammatory_effect_of_anemarsaponin_B_isolated_from_the_rhizomes_of_Anemarrhena_asphodeloides_in_LPS_induced_RAW_264_7_macrophages_is_mediated_by_negative_regulation_of_the_nuclear_factor_kappaB_and_p38_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(09)00163-X DB - PRIME DP - Unbound Medicine ER -