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Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells.
Exp Hematol. 2009 May; 37(5):629-40.EH

Abstract

OBJECTIVE

Mesenchymal stem cells (MSCs) can be isolated from umbilical cord Wharton's jelly (UC-MSC) and UC can be easily obtained, representing a noncontroversial source of MSCs. UC-MSCs are more primitive than other tissue sources. Previous studies showed that UC-MSCs were still viable and were not rejected 4 months after transplantation as xenografts without the need for immune suppression, indicating that they are favorable cell source for transplantation. In this study, UC-MSCs were induced to differentiate into endothelial-like cells and compared with bone marrow (BM)-MSCs for their endothelial differentiation potential.

MATERIALS AND METHODS

UC-MSCs and BM-MSCs were characterized for expression of MSC-specific markers and osteogenic, adipogenic, and chondrogenic differentiation. They were induced to differentiate into endothelial-like cells and analyzed for expression of the endothelial-specific markers and functions.

RESULTS

UC-MSCs and BM-MSCs showed similarities in expression of the MSC-specific markers and osteogenic, adipogenic, and chondrogenic differentiation. They showed similar low-density lipoprotein-uptaking capacity following endothelial differentiation. However, UC-MSCs had higher proliferative potential than BM-MSCs. Both real-time reverse transcription polymerase chain reaction and immunocytochemical analyses demonstrated that UC-MSCs had higher expression of the endothelial-specific markers than BM-MSCs following endothelial differentiation. Both Matrigel and coculture angiogenesis assays showed that UC-MSCs and BM-MSCs after endothelial differentiation were able to form the capillary network and differentiated UC-MSCs had significantly higher total tubule length, diameter, and area than differentiated BM-MSCs.

CONCLUSION

These results showed that UC-MSCs had higher endothelial differentiation potential than BM-MSCs. Therefore, UC-MSCs are more favorable choice than BM-MSCs for neovascularization of engineered tissues.

Authors+Show Affiliations

Multidisciplinary Research Center, Shantou University, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19375653

Citation

Chen, Ming-Yan, et al. "Endothelial Differentiation of Wharton's Jelly-derived Mesenchymal Stem Cells in Comparison With Bone Marrow-derived Mesenchymal Stem Cells." Experimental Hematology, vol. 37, no. 5, 2009, pp. 629-40.
Chen MY, Lie PC, Li ZL, et al. Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells. Exp Hematol. 2009;37(5):629-40.
Chen, M. Y., Lie, P. C., Li, Z. L., & Wei, X. (2009). Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells. Experimental Hematology, 37(5), 629-40. https://doi.org/10.1016/j.exphem.2009.02.003
Chen MY, et al. Endothelial Differentiation of Wharton's Jelly-derived Mesenchymal Stem Cells in Comparison With Bone Marrow-derived Mesenchymal Stem Cells. Exp Hematol. 2009;37(5):629-40. PubMed PMID: 19375653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells. AU - Chen,Ming-Yan, AU - Lie,Pu-Chang, AU - Li,Zhi-Ling, AU - Wei,Xing, PY - 2008/08/21/received PY - 2009/01/22/revised PY - 2009/02/04/accepted PY - 2009/4/21/entrez PY - 2009/4/21/pubmed PY - 2009/5/6/medline SP - 629 EP - 40 JF - Experimental hematology JO - Exp. Hematol. VL - 37 IS - 5 N2 - OBJECTIVE: Mesenchymal stem cells (MSCs) can be isolated from umbilical cord Wharton's jelly (UC-MSC) and UC can be easily obtained, representing a noncontroversial source of MSCs. UC-MSCs are more primitive than other tissue sources. Previous studies showed that UC-MSCs were still viable and were not rejected 4 months after transplantation as xenografts without the need for immune suppression, indicating that they are favorable cell source for transplantation. In this study, UC-MSCs were induced to differentiate into endothelial-like cells and compared with bone marrow (BM)-MSCs for their endothelial differentiation potential. MATERIALS AND METHODS: UC-MSCs and BM-MSCs were characterized for expression of MSC-specific markers and osteogenic, adipogenic, and chondrogenic differentiation. They were induced to differentiate into endothelial-like cells and analyzed for expression of the endothelial-specific markers and functions. RESULTS: UC-MSCs and BM-MSCs showed similarities in expression of the MSC-specific markers and osteogenic, adipogenic, and chondrogenic differentiation. They showed similar low-density lipoprotein-uptaking capacity following endothelial differentiation. However, UC-MSCs had higher proliferative potential than BM-MSCs. Both real-time reverse transcription polymerase chain reaction and immunocytochemical analyses demonstrated that UC-MSCs had higher expression of the endothelial-specific markers than BM-MSCs following endothelial differentiation. Both Matrigel and coculture angiogenesis assays showed that UC-MSCs and BM-MSCs after endothelial differentiation were able to form the capillary network and differentiated UC-MSCs had significantly higher total tubule length, diameter, and area than differentiated BM-MSCs. CONCLUSION: These results showed that UC-MSCs had higher endothelial differentiation potential than BM-MSCs. Therefore, UC-MSCs are more favorable choice than BM-MSCs for neovascularization of engineered tissues. SN - 1873-2399 UR - https://www.unboundmedicine.com/medline/citation/19375653/Endothelial_differentiation_of_Wharton's_jelly_derived_mesenchymal_stem_cells_in_comparison_with_bone_marrow_derived_mesenchymal_stem_cells_ DB - PRIME DP - Unbound Medicine ER -