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Increased interferon-gamma, interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris: host response but not bacterial species is the determinant factor of the disease.
J Dermatol Sci 2009; 55(1):47-52JD

Abstract

BACKGROUND

Acne vulgaris is a multifactorial inflammatory disease of the sebaceous follicles of the face and torso that frequently occurs in adolescence. Initially, acne starts as a non-inflammatory comedo. Subsequently, inflammatory reactions evolve to pustules, granulomas and cystic lesions. Many pathogenic mechanisms have been proposed including sebum excretion, obstruction of hair follicles, impaired keratinization of hair epithelium, bacterial overgrowth and immunological mechanisms; the role of Propionibacterium acnes (P. acnes) is particularly important. Facultative anaerobic gram-positive rods have been implicated in acne pathogenesis. However, the host immune response to P. acnes has not been as yet elucidated.

OBJECTIVES

The aim of the present study is to evaluate the importance of the immune response to P. acnes and the bacteriological factor in the pathogenesis of acne.

METHODS

P. acnes isolated from acne lesions and healthy volunteers skin were cultured. The peripheral blood mononuclear cells (PBMC) from acne patients or healthy volunteers were stimulated with viable P. acnes, and cytokine production was evaluated using RT-PCR and ELISA.

RESULTS

IFN-gamma, IL-12p40, and IL-8 mRNA and protein production were significantly increased in PBMC from acne patients compared to that from normal donors. However, different P. acnes species isolated from acne lesions or normal subjects showed no difference in cytokines production from acne patients and normal subjects PBMC.

CONCLUSIONS

The inflammatory response of acne appears to be attributable to P. acnes-induced host immune response rather than P. acnes strains from normal skin or acne lesions.

Authors+Show Affiliations

Department of Dermatology, Mie University, Graduate School of Medicine, Tsu, Mie, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19375895

Citation

Sugisaki, Hitomi, et al. "Increased Interferon-gamma, Interleukin-12p40 and IL-8 Production in Propionibacterium Acnes-treated Peripheral Blood Mononuclear Cells From Patient With Acne Vulgaris: Host Response but Not Bacterial Species Is the Determinant Factor of the Disease." Journal of Dermatological Science, vol. 55, no. 1, 2009, pp. 47-52.
Sugisaki H, Yamanaka K, Kakeda M, et al. Increased interferon-gamma, interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris: host response but not bacterial species is the determinant factor of the disease. J Dermatol Sci. 2009;55(1):47-52.
Sugisaki, H., Yamanaka, K., Kakeda, M., Kitagawa, H., Tanaka, K., Watanabe, K., ... Mizutani, H. (2009). Increased interferon-gamma, interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris: host response but not bacterial species is the determinant factor of the disease. Journal of Dermatological Science, 55(1), pp. 47-52. doi:10.1016/j.jdermsci.2009.02.015.
Sugisaki H, et al. Increased Interferon-gamma, Interleukin-12p40 and IL-8 Production in Propionibacterium Acnes-treated Peripheral Blood Mononuclear Cells From Patient With Acne Vulgaris: Host Response but Not Bacterial Species Is the Determinant Factor of the Disease. J Dermatol Sci. 2009;55(1):47-52. PubMed PMID: 19375895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased interferon-gamma, interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris: host response but not bacterial species is the determinant factor of the disease. AU - Sugisaki,Hitomi, AU - Yamanaka,Keiichi, AU - Kakeda,Masato, AU - Kitagawa,Hiroshi, AU - Tanaka,Kaori, AU - Watanabe,Kunitomo, AU - Gabazza,Esteban C, AU - Kurokawa,Ichiro, AU - Mizutani,Hitoshi, Y1 - 2009/04/17/ PY - 2008/12/01/received PY - 2009/02/25/revised PY - 2009/02/27/accepted PY - 2009/4/21/entrez PY - 2009/4/21/pubmed PY - 2009/8/22/medline SP - 47 EP - 52 JF - Journal of dermatological science JO - J. Dermatol. Sci. VL - 55 IS - 1 N2 - BACKGROUND: Acne vulgaris is a multifactorial inflammatory disease of the sebaceous follicles of the face and torso that frequently occurs in adolescence. Initially, acne starts as a non-inflammatory comedo. Subsequently, inflammatory reactions evolve to pustules, granulomas and cystic lesions. Many pathogenic mechanisms have been proposed including sebum excretion, obstruction of hair follicles, impaired keratinization of hair epithelium, bacterial overgrowth and immunological mechanisms; the role of Propionibacterium acnes (P. acnes) is particularly important. Facultative anaerobic gram-positive rods have been implicated in acne pathogenesis. However, the host immune response to P. acnes has not been as yet elucidated. OBJECTIVES: The aim of the present study is to evaluate the importance of the immune response to P. acnes and the bacteriological factor in the pathogenesis of acne. METHODS: P. acnes isolated from acne lesions and healthy volunteers skin were cultured. The peripheral blood mononuclear cells (PBMC) from acne patients or healthy volunteers were stimulated with viable P. acnes, and cytokine production was evaluated using RT-PCR and ELISA. RESULTS: IFN-gamma, IL-12p40, and IL-8 mRNA and protein production were significantly increased in PBMC from acne patients compared to that from normal donors. However, different P. acnes species isolated from acne lesions or normal subjects showed no difference in cytokines production from acne patients and normal subjects PBMC. CONCLUSIONS: The inflammatory response of acne appears to be attributable to P. acnes-induced host immune response rather than P. acnes strains from normal skin or acne lesions. SN - 1873-569X UR - https://www.unboundmedicine.com/medline/citation/19375895/Increased_interferon_gamma_interleukin_12p40_and_IL_8_production_in_Propionibacterium_acnes_treated_peripheral_blood_mononuclear_cells_from_patient_with_acne_vulgaris:_host_response_but_not_bacterial_species_is_the_determinant_factor_of_the_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-1811(09)00086-3 DB - PRIME DP - Unbound Medicine ER -