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Validated LC-MS/MS assay for the determination of felbinac: Application to a preclinical pharmacokinetics study of felbinac trometamol injection in rat.
J Pharm Biomed Anal. 2009 Aug 15; 50(1):41-5.JP

Abstract

A rapid and sensitive analytical method based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of felbinac in rat plasma, bile, urine, feces and tissue. Sample preparation involved liquid-liquid extraction with ethyl ether-dichloromethane (60:40, v/v). Chromatography of felbinac and the internal standard probenecid was performed within 2min on a Venusil MP C(18) column (100mmx4.6mm i.d., 5microm) with a mobile phase consisting of acetonitrile-5mM ammonium acetate containing 0.1% formic acid (pH 3.0) (80:20, v/v) at a flow rate of 1.2ml/min. Detection by electrospray negative ionization mass spectrometry and multiple-reaction monitoring of the transitions of felbinac at m/z 211.1-->167.0 and of probenecid at m/z 283.9-->239.9 was linear over the concentration range 5-5000ng/ml with a lower limit of quantitation of 5ng/ml using a sample volume of only 50microl. Intra- and inter-day precisions (as relative standard deviation, R.S.D.) were < or =7.3% and < or =6.4%, respectively, and accuracy (as relative error, R.E.) was in the range -2.1 to 7.4%. Recoveries and matrix effects were satisfactory in all the biological matrices examined. The method was applied to a preclinical pharmacokinetic study in rat involving a single intravenous injection of felbinac trometamol.

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Authors+Show Affiliations

Zhang C
Research Center for Drug Metabolism, Jilin University, Changchun, PR China.
Wang L
No affiliation info available
Yang W
No affiliation info available
Wang X
No affiliation info available
Fawcett JP
No affiliation info available
Sun Y
No affiliation info available
Gu J
No affiliation info available

MeSH

AnimalsAnti-Inflammatory Agents, Non-SteroidalChromatography, High Pressure LiquidPhenylacetatesRatsReference StandardsReproducibility of ResultsSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19376665

Citation

Zhang, Chao, et al. "Validated LC-MS/MS Assay for the Determination of Felbinac: Application to a Preclinical Pharmacokinetics Study of Felbinac Trometamol Injection in Rat." Journal of Pharmaceutical and Biomedical Analysis, vol. 50, no. 1, 2009, pp. 41-5.
Zhang C, Wang L, Yang W, et al. Validated LC-MS/MS assay for the determination of felbinac: Application to a preclinical pharmacokinetics study of felbinac trometamol injection in rat. J Pharm Biomed Anal. 2009;50(1):41-5.
Zhang, C., Wang, L., Yang, W., Wang, X., Fawcett, J. P., Sun, Y., & Gu, J. (2009). Validated LC-MS/MS assay for the determination of felbinac: Application to a preclinical pharmacokinetics study of felbinac trometamol injection in rat. Journal of Pharmaceutical and Biomedical Analysis, 50(1), 41-5. https://doi.org/10.1016/j.jpba.2009.03.019
Zhang C, et al. Validated LC-MS/MS Assay for the Determination of Felbinac: Application to a Preclinical Pharmacokinetics Study of Felbinac Trometamol Injection in Rat. J Pharm Biomed Anal. 2009 Aug 15;50(1):41-5. PubMed PMID: 19376665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated LC-MS/MS assay for the determination of felbinac: Application to a preclinical pharmacokinetics study of felbinac trometamol injection in rat. AU - Zhang,Chao, AU - Wang,Lu, AU - Yang,Wei, AU - Wang,Xisha, AU - Fawcett,J Paul, AU - Sun,Yantong, AU - Gu,Jingkai, Y1 - 2009/03/26/ PY - 2009/02/21/received PY - 2009/03/17/revised PY - 2009/03/18/accepted PY - 2009/4/21/entrez PY - 2009/4/21/pubmed PY - 2009/8/6/medline SP - 41 EP - 5 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 50 IS - 1 N2 - A rapid and sensitive analytical method based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of felbinac in rat plasma, bile, urine, feces and tissue. Sample preparation involved liquid-liquid extraction with ethyl ether-dichloromethane (60:40, v/v). Chromatography of felbinac and the internal standard probenecid was performed within 2min on a Venusil MP C(18) column (100mmx4.6mm i.d., 5microm) with a mobile phase consisting of acetonitrile-5mM ammonium acetate containing 0.1% formic acid (pH 3.0) (80:20, v/v) at a flow rate of 1.2ml/min. Detection by electrospray negative ionization mass spectrometry and multiple-reaction monitoring of the transitions of felbinac at m/z 211.1-->167.0 and of probenecid at m/z 283.9-->239.9 was linear over the concentration range 5-5000ng/ml with a lower limit of quantitation of 5ng/ml using a sample volume of only 50microl. Intra- and inter-day precisions (as relative standard deviation, R.S.D.) were < or =7.3% and < or =6.4%, respectively, and accuracy (as relative error, R.E.) was in the range -2.1 to 7.4%. Recoveries and matrix effects were satisfactory in all the biological matrices examined. The method was applied to a preclinical pharmacokinetic study in rat involving a single intravenous injection of felbinac trometamol. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/19376665/Validated_LC_MS/MS_assay_for_the_determination_of_felbinac:_Application_to_a_preclinical_pharmacokinetics_study_of_felbinac_trometamol_injection_in_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(09)00196-4 DB - PRIME DP - Unbound Medicine ER -