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Genetic evidence for key roles of decorin and biglycan in dentin mineralization.
Matrix Biol. 2009 Apr; 28(3):129-36.MB

Abstract

Targeted disruption of the dentin sialophosphoprotein (DSPP) gene in the mice (Dspp(-/-)) results in dentin mineralization defects with enlarged predentin phenotype similar to human dentinogenesis imperfecta type III. Using DSPP/biglycan (Dspp(-/-)Bgn(-/0)) and DSPP/decorin (Dspp(-/-)Dcn(-/-)) double knockout mice, here we determined that the enlarged predentin layer in Dspp(-/-) teeth is rescued in the absence of decorin, but not in the absence of biglycan. However, Fourier transform infrared (FTIR) spectroscopy analysis reveals similar hypomineralization of dentin in both Dspp(-/-)Bgn(-/0) and Dspp(-/-)Dcn(-/-) teeth. Atomic force microscopy (AFM) analysis of collagen fibrils in dentin shows subtle differences in the collagen fibril morphology in these genotypes. The reduction of enlarged predentin in Dspp(-/-)Dcn(-/-) mice suggests that the elevated level of decorin in Dspp(-/-) predentin interferes with the mineralization process at the dentin mineralization front. On the other hand, the lack of DSPP and biglycan leads to the increased number of calcospherites in Dspp(-/-)Bgn(-/0) predentin, suggesting that a failure in coalescence of calcospherites was augmented in Dspp(-/-)Bgn(-/0) teeth as compared to Dspp(-/-) teeth. These findings indicate that normal expression of small leucine rich proteoglycans, such as biglycan and decorin, plays an important role in the highly orchestrated process of dentin mineralization.

Authors+Show Affiliations

Functional Genomics Section, Laboratory of Cell and Developmental Biology, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

19379665

Citation

Haruyama, Naoto, et al. "Genetic Evidence for Key Roles of Decorin and Biglycan in Dentin Mineralization." Matrix Biology : Journal of the International Society for Matrix Biology, vol. 28, no. 3, 2009, pp. 129-36.
Haruyama N, Sreenath TL, Suzuki S, et al. Genetic evidence for key roles of decorin and biglycan in dentin mineralization. Matrix Biol. 2009;28(3):129-36.
Haruyama, N., Sreenath, T. L., Suzuki, S., Yao, X., Wang, Z., Wang, Y., Honeycutt, C., Iozzo, R. V., Young, M. F., & Kulkarni, A. B. (2009). Genetic evidence for key roles of decorin and biglycan in dentin mineralization. Matrix Biology : Journal of the International Society for Matrix Biology, 28(3), 129-36. https://doi.org/10.1016/j.matbio.2009.01.005
Haruyama N, et al. Genetic Evidence for Key Roles of Decorin and Biglycan in Dentin Mineralization. Matrix Biol. 2009;28(3):129-36. PubMed PMID: 19379665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic evidence for key roles of decorin and biglycan in dentin mineralization. AU - Haruyama,Naoto, AU - Sreenath,Taduru L, AU - Suzuki,Shigeki, AU - Yao,Xiaomei, AU - Wang,Zhigang, AU - Wang,Yong, AU - Honeycutt,Cherlita, AU - Iozzo,Renato V, AU - Young,Marian F, AU - Kulkarni,Ashok B, Y1 - 2009/02/12/ PY - 2008/08/01/received PY - 2008/12/25/revised PY - 2009/01/23/accepted PY - 2009/4/22/entrez PY - 2009/4/22/pubmed PY - 2009/6/30/medline SP - 129 EP - 36 JF - Matrix biology : journal of the International Society for Matrix Biology JO - Matrix Biol VL - 28 IS - 3 N2 - Targeted disruption of the dentin sialophosphoprotein (DSPP) gene in the mice (Dspp(-/-)) results in dentin mineralization defects with enlarged predentin phenotype similar to human dentinogenesis imperfecta type III. Using DSPP/biglycan (Dspp(-/-)Bgn(-/0)) and DSPP/decorin (Dspp(-/-)Dcn(-/-)) double knockout mice, here we determined that the enlarged predentin layer in Dspp(-/-) teeth is rescued in the absence of decorin, but not in the absence of biglycan. However, Fourier transform infrared (FTIR) spectroscopy analysis reveals similar hypomineralization of dentin in both Dspp(-/-)Bgn(-/0) and Dspp(-/-)Dcn(-/-) teeth. Atomic force microscopy (AFM) analysis of collagen fibrils in dentin shows subtle differences in the collagen fibril morphology in these genotypes. The reduction of enlarged predentin in Dspp(-/-)Dcn(-/-) mice suggests that the elevated level of decorin in Dspp(-/-) predentin interferes with the mineralization process at the dentin mineralization front. On the other hand, the lack of DSPP and biglycan leads to the increased number of calcospherites in Dspp(-/-)Bgn(-/0) predentin, suggesting that a failure in coalescence of calcospherites was augmented in Dspp(-/-)Bgn(-/0) teeth as compared to Dspp(-/-) teeth. These findings indicate that normal expression of small leucine rich proteoglycans, such as biglycan and decorin, plays an important role in the highly orchestrated process of dentin mineralization. SN - 1569-1802 UR - https://www.unboundmedicine.com/medline/citation/19379665/Genetic_evidence_for_key_roles_of_decorin_and_biglycan_in_dentin_mineralization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0945-053X(09)00007-9 DB - PRIME DP - Unbound Medicine ER -