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Varying abilities of recombinant polypeptides from different regions of hepatitis E virus ORF2 and ORF3 to detect anti-HEV immunoglobulin M.
J Med Virol. 2009 Jun; 81(6):1052-61.JM

Abstract

Following infection with hepatitis E virus (HEV), anti-HEV immunoglobulin (Ig) M is thought to develop before anti-HEV IgG and to be a better marker for differentiating between the acute and convalescent phases of infection. In order to select polypeptides for improved detection of anti-HEV IgM, six and three overlapping polypeptides from open reading frames (ORFs) 2 and 3, respectively, of HEV genotypes 1 and 4 were expressed as fusion proteins in Escherichia coli. The reactivities of the polypeptides with anti-HEV IgM were evaluated using immunoblotting and enzyme immunoassays (EIAs). The data indicated that polypeptides from the N-terminus of ORF3 and middle region of ORF2 were weakly or not reactive with anti-HEV IgM, while those from the remaining regions of ORF2 and ORF3 contained reactive epitopes. Anti-HEV IgM against the N- or C-terminus of ORF2 appeared earlier and disappeared faster than that against polypeptides from the C-terminus of ORF3, based on serum samples from rhesus monkeys infected experimentally, and from patients infected naturally, with HEV. The N- and C-terminal polypeptides from ORF2 complemented one another in detecting anti-HEV IgM and EIA sensitivity was improved significantly with a combination of these polypeptides. The reactivities of ORF2 polypeptides from genotypes 1 and 4 were similar but that of ORF3 differed with sera from monkeys infected by the two genotypes. Thus, a combination of N- and C-terminal polypeptides of ORF2 from one genotype may be effective in EIAs to detect anti-HEV IgM.

Authors+Show Affiliations

College of Life Science, Jilin University, Changchun, Jilin Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19382255

Citation

Ma, Hongxia, et al. "Varying Abilities of Recombinant Polypeptides From Different Regions of Hepatitis E Virus ORF2 and ORF3 to Detect anti-HEV Immunoglobulin M." Journal of Medical Virology, vol. 81, no. 6, 2009, pp. 1052-61.
Ma H, Song X, Li Z, et al. Varying abilities of recombinant polypeptides from different regions of hepatitis E virus ORF2 and ORF3 to detect anti-HEV immunoglobulin M. J Med Virol. 2009;81(6):1052-61.
Ma, H., Song, X., Li, Z., Harrison, T. J., Zhang, H., Huang, W., Hao, W., Kong, W., & Wang, Y. (2009). Varying abilities of recombinant polypeptides from different regions of hepatitis E virus ORF2 and ORF3 to detect anti-HEV immunoglobulin M. Journal of Medical Virology, 81(6), 1052-61. https://doi.org/10.1002/jmv.21484
Ma H, et al. Varying Abilities of Recombinant Polypeptides From Different Regions of Hepatitis E Virus ORF2 and ORF3 to Detect anti-HEV Immunoglobulin M. J Med Virol. 2009;81(6):1052-61. PubMed PMID: 19382255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Varying abilities of recombinant polypeptides from different regions of hepatitis E virus ORF2 and ORF3 to detect anti-HEV immunoglobulin M. AU - Ma,Hongxia, AU - Song,Xiaoguo, AU - Li,Zhuo, AU - Harrison,Tim J, AU - Zhang,Heqiu, AU - Huang,Weijin, AU - Hao,Wa, AU - Kong,Wei, AU - Wang,Youchun, PY - 2009/4/22/entrez PY - 2009/4/22/pubmed PY - 2009/6/9/medline SP - 1052 EP - 61 JF - Journal of medical virology JO - J Med Virol VL - 81 IS - 6 N2 - Following infection with hepatitis E virus (HEV), anti-HEV immunoglobulin (Ig) M is thought to develop before anti-HEV IgG and to be a better marker for differentiating between the acute and convalescent phases of infection. In order to select polypeptides for improved detection of anti-HEV IgM, six and three overlapping polypeptides from open reading frames (ORFs) 2 and 3, respectively, of HEV genotypes 1 and 4 were expressed as fusion proteins in Escherichia coli. The reactivities of the polypeptides with anti-HEV IgM were evaluated using immunoblotting and enzyme immunoassays (EIAs). The data indicated that polypeptides from the N-terminus of ORF3 and middle region of ORF2 were weakly or not reactive with anti-HEV IgM, while those from the remaining regions of ORF2 and ORF3 contained reactive epitopes. Anti-HEV IgM against the N- or C-terminus of ORF2 appeared earlier and disappeared faster than that against polypeptides from the C-terminus of ORF3, based on serum samples from rhesus monkeys infected experimentally, and from patients infected naturally, with HEV. The N- and C-terminal polypeptides from ORF2 complemented one another in detecting anti-HEV IgM and EIA sensitivity was improved significantly with a combination of these polypeptides. The reactivities of ORF2 polypeptides from genotypes 1 and 4 were similar but that of ORF3 differed with sera from monkeys infected by the two genotypes. Thus, a combination of N- and C-terminal polypeptides of ORF2 from one genotype may be effective in EIAs to detect anti-HEV IgM. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/19382255/Varying_abilities_of_recombinant_polypeptides_from_different_regions_of_hepatitis_E_virus_ORF2_and_ORF3_to_detect_anti_HEV_immunoglobulin_M_ L2 - https://doi.org/10.1002/jmv.21484 DB - PRIME DP - Unbound Medicine ER -