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Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections.
J Med Virol. 2009 Jun; 81(6):1102-8.JM

Abstract

Monkeypox virus (MPXV) causes a smallpox-like disease in non-human primates and humans. This infection is endemic to central and western Africa. MPXV is divided into two genetically different groups, Congo Basin and West African MPXV, with the former being the more virulent. A real-time quantitative MPXV genome amplification system was developed for the diagnosis of MPXV infections using loop-mediated isothermal amplification (LAMP) technology. Primers used for genome amplification of Congo Basin (C-LAMP), West African (W-LAMP), and both Congo Basin and West African (COM-LAMP) MPXV by LAMP were designed according to the nucleotide sequences of the Congo Basin-specific D14L gene, the West African-specific partial ATI gene, and the partial ATI gene that is shared by both groups, respectively. The sensitivity and specificity of the LAMP were evaluated with nested PCR using peripheral blood and throat swab specimens collected from Congo Basin MPXV or West African MPXV-infected monkeys. The sensitivity and specificity of COM-LAMP, C-LAMP, and W-LAMP were 80% (45/56) and 100% (64/64); 79% (19/24) and 100% (24/24); and 72% (23/32) and 100% (40/40), respectively. The viremia level determined by LAMP assays increased with increases in the severity of the monkeypox-associated symptoms. The newly developed LAMP assay was confirmed to be a rapid, quantifiable, and highly sensitive and specific system effective in the diagnosis of MPXV infections. The LAMP assays made it possible to discriminate between Congo Basin and West African MPXV. The LAMP developed in this study is useful not only for diagnosis of but also for the assessment of MPXV infections.

Authors+Show Affiliations

Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19382264

Citation

Iizuka, Itoe, et al. "Loop-mediated Isothermal Amplification-based Diagnostic Assay for Monkeypox Virus Infections." Journal of Medical Virology, vol. 81, no. 6, 2009, pp. 1102-8.
Iizuka I, Saijo M, Shiota T, et al. Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections. J Med Virol. 2009;81(6):1102-8.
Iizuka, I., Saijo, M., Shiota, T., Ami, Y., Suzaki, Y., Nagata, N., Hasegawa, H., Sakai, K., Fukushi, S., Mizutani, T., Ogata, M., Nakauchi, M., Kurane, I., Mizuguchi, M., & Morikawa, S. (2009). Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections. Journal of Medical Virology, 81(6), 1102-8. https://doi.org/10.1002/jmv.21494
Iizuka I, et al. Loop-mediated Isothermal Amplification-based Diagnostic Assay for Monkeypox Virus Infections. J Med Virol. 2009;81(6):1102-8. PubMed PMID: 19382264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections. AU - Iizuka,Itoe, AU - Saijo,Masayuki, AU - Shiota,Tomoyuki, AU - Ami,Yasushi, AU - Suzaki,Yuriko, AU - Nagata,Noriyo, AU - Hasegawa,Hideki, AU - Sakai,Kouji, AU - Fukushi,Shuetsu, AU - Mizutani,Tetsuya, AU - Ogata,Momoko, AU - Nakauchi,Mina, AU - Kurane,Ichiro, AU - Mizuguchi,Masashi, AU - Morikawa,Shigeru, PY - 2009/4/22/entrez PY - 2009/4/22/pubmed PY - 2009/6/9/medline SP - 1102 EP - 8 JF - Journal of medical virology JO - J Med Virol VL - 81 IS - 6 N2 - Monkeypox virus (MPXV) causes a smallpox-like disease in non-human primates and humans. This infection is endemic to central and western Africa. MPXV is divided into two genetically different groups, Congo Basin and West African MPXV, with the former being the more virulent. A real-time quantitative MPXV genome amplification system was developed for the diagnosis of MPXV infections using loop-mediated isothermal amplification (LAMP) technology. Primers used for genome amplification of Congo Basin (C-LAMP), West African (W-LAMP), and both Congo Basin and West African (COM-LAMP) MPXV by LAMP were designed according to the nucleotide sequences of the Congo Basin-specific D14L gene, the West African-specific partial ATI gene, and the partial ATI gene that is shared by both groups, respectively. The sensitivity and specificity of the LAMP were evaluated with nested PCR using peripheral blood and throat swab specimens collected from Congo Basin MPXV or West African MPXV-infected monkeys. The sensitivity and specificity of COM-LAMP, C-LAMP, and W-LAMP were 80% (45/56) and 100% (64/64); 79% (19/24) and 100% (24/24); and 72% (23/32) and 100% (40/40), respectively. The viremia level determined by LAMP assays increased with increases in the severity of the monkeypox-associated symptoms. The newly developed LAMP assay was confirmed to be a rapid, quantifiable, and highly sensitive and specific system effective in the diagnosis of MPXV infections. The LAMP assays made it possible to discriminate between Congo Basin and West African MPXV. The LAMP developed in this study is useful not only for diagnosis of but also for the assessment of MPXV infections. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/19382264/Loop_mediated_isothermal_amplification_based_diagnostic_assay_for_monkeypox_virus_infections_ L2 - https://doi.org/10.1002/jmv.21494 DB - PRIME DP - Unbound Medicine ER -