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Activin A, activin receptor type II, nodal, and cripto mRNA are expressed by eutopic and ectopic endometrium in women with ovarian endometriosis.
Reprod Sci. 2009 Aug; 16(8):727-33.RS

Abstract

Activin A is a dimeric protein that regulates endometrial functions by signaling at its receptors, namely type I (ActRI) and type II (ActRII). Nodal is an activin competitor that requires the coreceptor cripto to assemble its signaling pathway through ActRI and ActRII. In the current study, we evaluated the expression of activin A, ActRII, nodal, and cripto in eutopic and ectopic endometrium collected from women with ovarian endometrioma (n = 15) and in eutopic endometrium of healthy participants (n = 15). Eutopic endometrial samples were evaluated according to the stage of menstrual cycle. Total RNA was extracted from tissue homogenates and analyzed by real-time polymerase chain reaction (PCR). Activin A messenger RNA (mRNA) expression in eutopic endometrium of patients with endometriosis was significantly higher than in controls (P < .001) with a 10.2-fold and 7.3-fold increase in the proliferative and secretory phases, respectively. ActRII and nodal mRNA expression were found to be similar in patients with and without endometriosis, while cripto mRNA was markedly lower in eutopic (fold change = 0.03 at proliferative phase, P < .001) and ectopic endometrium (fold change = 0.14, P < .001) of women with endometriosis compared with eutopic endometrium from healthy controls. In conclusion, the altered endometrial expression of activin A and cripto during the menstrual cycle and the differences observed in the endometriotic tissue support the involvement of the activin system in endometrial changes of women with endometriosis.

Authors+Show Affiliations

Department of Pediatrics, Obstetrics and Reproductive Medicine, Section of Obstetrics and Gynecology, University of Siena, Siena, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19386982

Citation

Torres, Paulo B., et al. "Activin A, Activin Receptor Type II, Nodal, and Cripto mRNA Are Expressed By Eutopic and Ectopic Endometrium in Women With Ovarian Endometriosis." Reproductive Sciences (Thousand Oaks, Calif.), vol. 16, no. 8, 2009, pp. 727-33.
Torres PB, Florio P, Galleri L, et al. Activin A, activin receptor type II, nodal, and cripto mRNA are expressed by eutopic and ectopic endometrium in women with ovarian endometriosis. Reprod Sci. 2009;16(8):727-33.
Torres, P. B., Florio, P., Galleri, L., Reis, F. M., Borges, L. E., & Petraglia, F. (2009). Activin A, activin receptor type II, nodal, and cripto mRNA are expressed by eutopic and ectopic endometrium in women with ovarian endometriosis. Reproductive Sciences (Thousand Oaks, Calif.), 16(8), 727-33. https://doi.org/10.1177/1933719109334967
Torres PB, et al. Activin A, Activin Receptor Type II, Nodal, and Cripto mRNA Are Expressed By Eutopic and Ectopic Endometrium in Women With Ovarian Endometriosis. Reprod Sci. 2009;16(8):727-33. PubMed PMID: 19386982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activin A, activin receptor type II, nodal, and cripto mRNA are expressed by eutopic and ectopic endometrium in women with ovarian endometriosis. AU - Torres,Paulo B, AU - Florio,Pasquale, AU - Galleri,Letizia, AU - Reis,Fernando M, AU - Borges,Lavinia E, AU - Petraglia,Felice, Y1 - 2009/04/22/ PY - 2009/4/24/entrez PY - 2009/4/24/pubmed PY - 2009/9/18/medline SP - 727 EP - 33 JF - Reproductive sciences (Thousand Oaks, Calif.) JO - Reprod Sci VL - 16 IS - 8 N2 - Activin A is a dimeric protein that regulates endometrial functions by signaling at its receptors, namely type I (ActRI) and type II (ActRII). Nodal is an activin competitor that requires the coreceptor cripto to assemble its signaling pathway through ActRI and ActRII. In the current study, we evaluated the expression of activin A, ActRII, nodal, and cripto in eutopic and ectopic endometrium collected from women with ovarian endometrioma (n = 15) and in eutopic endometrium of healthy participants (n = 15). Eutopic endometrial samples were evaluated according to the stage of menstrual cycle. Total RNA was extracted from tissue homogenates and analyzed by real-time polymerase chain reaction (PCR). Activin A messenger RNA (mRNA) expression in eutopic endometrium of patients with endometriosis was significantly higher than in controls (P < .001) with a 10.2-fold and 7.3-fold increase in the proliferative and secretory phases, respectively. ActRII and nodal mRNA expression were found to be similar in patients with and without endometriosis, while cripto mRNA was markedly lower in eutopic (fold change = 0.03 at proliferative phase, P < .001) and ectopic endometrium (fold change = 0.14, P < .001) of women with endometriosis compared with eutopic endometrium from healthy controls. In conclusion, the altered endometrial expression of activin A and cripto during the menstrual cycle and the differences observed in the endometriotic tissue support the involvement of the activin system in endometrial changes of women with endometriosis. SN - 1933-7205 UR - https://www.unboundmedicine.com/medline/citation/19386982/Activin_A_activin_receptor_type_II_nodal_and_cripto_mRNA_are_expressed_by_eutopic_and_ectopic_endometrium_in_women_with_ovarian_endometriosis_ L2 - http://journals.sagepub.com/doi/full/10.1177/1933719109334967?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -