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Overexpression of seleno-glutathione peroxidase by gene transfer enhances the resistance of T47D human breast cells to clastogenic oxidants.
J Biol Chem 1991; 266(31):20752-60JB

Abstract

The role of seleno-glutathione peroxidase (GSHPx; EC 1.11.1.9) in the cellular defense against oxidative stress was selectively investigated in novel cell models. Expression vectors designed to overexpress human GSHPx efficiently in a broad range of mammalian cells were used to transfect T47D human breast cells which contain very low levels of endogenous GSHPx. Several stable transfectants expressing GSHPx to various extents, up to 10-100 times more than parental cells, were isolated and characterized. Growth inhibition kinetics following transient exposure to increasing concentrations of H2O2, cumene hydroperoxide or menadione (an intracellular source of free radicals and reactive oxygen intermediates) showed that transfectants overexpressing GSHPx were considerably more resistant than control T47D cell derivatives to each of these oxidants. A sensitive DNA end-labeling procedure was used as a novel approach to compare relative extents of DNA strand breakage in these cells. In contrast to the extensive DNA damage induced in control transfectants by 1-h exposure to cytotoxic concentrations of menadione, the extent of DNA breakage detected in GSHPx-rich transfectants was remarkably reduced (6- to 9-fold, p less than 0.005).

Authors+Show Affiliations

Department of Medicine, Centre Hospitalier, Université Laval Research Center, Ste-Foy, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1939125

Citation

Mirault, M E., et al. "Overexpression of Seleno-glutathione Peroxidase By Gene Transfer Enhances the Resistance of T47D Human Breast Cells to Clastogenic Oxidants." The Journal of Biological Chemistry, vol. 266, no. 31, 1991, pp. 20752-60.
Mirault ME, Tremblay A, Beaudoin N, et al. Overexpression of seleno-glutathione peroxidase by gene transfer enhances the resistance of T47D human breast cells to clastogenic oxidants. J Biol Chem. 1991;266(31):20752-60.
Mirault, M. E., Tremblay, A., Beaudoin, N., & Tremblay, M. (1991). Overexpression of seleno-glutathione peroxidase by gene transfer enhances the resistance of T47D human breast cells to clastogenic oxidants. The Journal of Biological Chemistry, 266(31), pp. 20752-60.
Mirault ME, et al. Overexpression of Seleno-glutathione Peroxidase By Gene Transfer Enhances the Resistance of T47D Human Breast Cells to Clastogenic Oxidants. J Biol Chem. 1991 Nov 5;266(31):20752-60. PubMed PMID: 1939125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of seleno-glutathione peroxidase by gene transfer enhances the resistance of T47D human breast cells to clastogenic oxidants. AU - Mirault,M E, AU - Tremblay,A, AU - Beaudoin,N, AU - Tremblay,M, PY - 1991/11/5/pubmed PY - 1991/11/5/medline PY - 1991/11/5/entrez SP - 20752 EP - 60 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 266 IS - 31 N2 - The role of seleno-glutathione peroxidase (GSHPx; EC 1.11.1.9) in the cellular defense against oxidative stress was selectively investigated in novel cell models. Expression vectors designed to overexpress human GSHPx efficiently in a broad range of mammalian cells were used to transfect T47D human breast cells which contain very low levels of endogenous GSHPx. Several stable transfectants expressing GSHPx to various extents, up to 10-100 times more than parental cells, were isolated and characterized. Growth inhibition kinetics following transient exposure to increasing concentrations of H2O2, cumene hydroperoxide or menadione (an intracellular source of free radicals and reactive oxygen intermediates) showed that transfectants overexpressing GSHPx were considerably more resistant than control T47D cell derivatives to each of these oxidants. A sensitive DNA end-labeling procedure was used as a novel approach to compare relative extents of DNA strand breakage in these cells. In contrast to the extensive DNA damage induced in control transfectants by 1-h exposure to cytotoxic concentrations of menadione, the extent of DNA breakage detected in GSHPx-rich transfectants was remarkably reduced (6- to 9-fold, p less than 0.005). SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/1939125/Overexpression_of_seleno_glutathione_peroxidase_by_gene_transfer_enhances_the_resistance_of_T47D_human_breast_cells_to_clastogenic_oxidants_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=1939125 DB - PRIME DP - Unbound Medicine ER -