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"Physiologic ICSI": hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality.
Fertil Steril. 2010 Feb; 93(2):598-604.FS

Abstract

OBJECTIVE

To evaluate the role of hyaluronic acid (HA) for sperm selection before intracytoplasmic sperm injection (ICSI).

DESIGN

Three prospective studies.

SETTING

Private assisted reproduction center in Italy.

PATIENT(S)

Study 1: 20 men. Study 2: 15 men. Study 3: 206 couples treated with ICSI on a limited number of oocytes per patient (1-3) in accordance with Italian IVF law.

INTERVENTION(S)

Study 1: determination of sperm DNA fragmentation of HA-bound spermatozoa versus spermatozoa in polyvinylpyrrolidone (PVP). Study 2: assessment of nuclear morphology of HA-bound spermatozoa versus spermatozoa in PVP. Study 3: randomized study comparing conventional PVP-ICSI to ICSI in which the spermatozoa are selected for their capacity to bind to HA (HA-ICSI).

MAIN OUTCOME MEASURE(S)

Study 1: sperm DNA fragmentation rate. Study 2: sperm nucleus normalcy rate according to motile sperm organellar morphology examination criteria. Study 3: fertilization, embryo quality and development, and implantation and pregnancy.

RESULT(S)

Spematozoa bound to HA show a significant reduction in DNA fragmentation (study 1) and a significant improvement in nucleus normalcy (study 2) compared with spermatozoa immersed in PVP. Furthermore, injection of HA-bound spermatozoa (HA-ICSI) significantly improves embryo quality and development (study 3).

CONCLUSION(S)

Hyaluronic acid may optimize ICSI outcome by favoring selection of spermatozoa without DNA fragmentation and with normal nucleus. Furthermore, HA may also be used to speed up the selection of spermatozoa with normal nucleus during intracytoplasmic morphologically selected sperm injection (IMSI).

Authors+Show Affiliations

Reproductive Medicine Unit, GynePro Medical Centers, Via T. Cremona, 8, 40137, Italy. l.parmegiani@gynepro.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

19393999

Citation

Parmegiani, Lodovico, et al. ""Physiologic ICSI": Hyaluronic Acid (HA) Favors Selection of Spermatozoa Without DNA Fragmentation and With Normal Nucleus, Resulting in Improvement of Embryo Quality." Fertility and Sterility, vol. 93, no. 2, 2010, pp. 598-604.
Parmegiani L, Cognigni GE, Bernardi S, et al. "Physiologic ICSI": hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil Steril. 2010;93(2):598-604.
Parmegiani, L., Cognigni, G. E., Bernardi, S., Troilo, E., Ciampaglia, W., & Filicori, M. (2010). "Physiologic ICSI": hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertility and Sterility, 93(2), 598-604. https://doi.org/10.1016/j.fertnstert.2009.03.033
Parmegiani L, et al. "Physiologic ICSI": Hyaluronic Acid (HA) Favors Selection of Spermatozoa Without DNA Fragmentation and With Normal Nucleus, Resulting in Improvement of Embryo Quality. Fertil Steril. 2010;93(2):598-604. PubMed PMID: 19393999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - "Physiologic ICSI": hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. AU - Parmegiani,Lodovico, AU - Cognigni,Graciela Estela, AU - Bernardi,Silvia, AU - Troilo,Enzo, AU - Ciampaglia,Walter, AU - Filicori,Marco, Y1 - 2009/04/25/ PY - 2008/12/22/received PY - 2009/02/19/revised PY - 2009/03/04/accepted PY - 2009/4/28/entrez PY - 2009/4/28/pubmed PY - 2010/2/25/medline SP - 598 EP - 604 JF - Fertility and sterility JO - Fertil. Steril. VL - 93 IS - 2 N2 - OBJECTIVE: To evaluate the role of hyaluronic acid (HA) for sperm selection before intracytoplasmic sperm injection (ICSI). DESIGN: Three prospective studies. SETTING: Private assisted reproduction center in Italy. PATIENT(S): Study 1: 20 men. Study 2: 15 men. Study 3: 206 couples treated with ICSI on a limited number of oocytes per patient (1-3) in accordance with Italian IVF law. INTERVENTION(S): Study 1: determination of sperm DNA fragmentation of HA-bound spermatozoa versus spermatozoa in polyvinylpyrrolidone (PVP). Study 2: assessment of nuclear morphology of HA-bound spermatozoa versus spermatozoa in PVP. Study 3: randomized study comparing conventional PVP-ICSI to ICSI in which the spermatozoa are selected for their capacity to bind to HA (HA-ICSI). MAIN OUTCOME MEASURE(S): Study 1: sperm DNA fragmentation rate. Study 2: sperm nucleus normalcy rate according to motile sperm organellar morphology examination criteria. Study 3: fertilization, embryo quality and development, and implantation and pregnancy. RESULT(S): Spematozoa bound to HA show a significant reduction in DNA fragmentation (study 1) and a significant improvement in nucleus normalcy (study 2) compared with spermatozoa immersed in PVP. Furthermore, injection of HA-bound spermatozoa (HA-ICSI) significantly improves embryo quality and development (study 3). CONCLUSION(S): Hyaluronic acid may optimize ICSI outcome by favoring selection of spermatozoa without DNA fragmentation and with normal nucleus. Furthermore, HA may also be used to speed up the selection of spermatozoa with normal nucleus during intracytoplasmic morphologically selected sperm injection (IMSI). SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/19393999/"Physiologic_ICSI":_hyaluronic_acid__HA__favors_selection_of_spermatozoa_without_DNA_fragmentation_and_with_normal_nucleus_resulting_in_improvement_of_embryo_quality_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(09)00598-6 DB - PRIME DP - Unbound Medicine ER -