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Attenuation of liver ischemia/reperfusion induced apoptosis by epigallocatechin-3-gallate via down-regulation of NF-kappaB and c-Jun expression.
J Surg Res. 2010 Apr; 159(2):720-8.JS

Abstract

INTRODUCTION

Hepatic ischemia/reperfusion (I/R) activates Kupffer cells and initiates severe oxidative stress with enhanced production of reactive oxygen species (ROS) and tumor necrosis factor-alpha (TNF-alpha). ROS and TNF-alpha mediate the expression of nuclear factors and kinases, activating the signal transduction pathway, and triggering apoptosis. The aim of our study was to evaluate the potential protective effect of (-)-epigallocatechin-3-gallate (EGCG) administration in inhibition of apoptosis by attenuating the expression of NF-kappaB, c-Jun, and caspase-3 in a model of severe hepatic I/R.

MATERIALS AND METHODS

Thirty Wistar rats were allocated into three groups. Sham operation, I/R, and I/R-EGCG 50mg/kg. Hepatic ischemia was induced for 60min by Pringle's maneuver. Malondialdehyde (MDA), myeloperoxidase (MPO), light histology, scanning electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and immunocytochemistry for NF-kappaB, c-Jun, caspase-3, analysis on liver specimens and aspartate (AST), and alanine (ALT) transferases analysis in serum, were performed 120min after reperfusion.

RESULTS

Apoptosis as indicated by TUNEL and caspase-3 was widely expressed in the I/R group but very limited in the EGCG treated group. Liver was stained positive for NF-kappaB and c-Jun in the I/R group but failed to be stained positive in the EGCG treated group. MDA, MPO, AST, and ALT showed marked increase in the I/R group and significant decrease in EGCG treated group. Significant alterations of liver specimens were observed by light histology and transmission electron microscopy whilst pretreatment with EGCG resulted in parenchymal preservation.

CONCLUSIONS

Administration of EGCG is likely to inhibit I/R-induced apoptosis and protect liver by down-regulating NF-kappaB and c-Jun signal transduction pathways.

Authors+Show Affiliations

Department of Transplant Surgery, Medical School, Aristotle University and Hippokration General Hospital, Thessaloniki, Greece. dgiak@auth.gr <dgiak@auth.gr>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19394642

Citation

Giakoustidis, Dimitrios E., et al. "Attenuation of Liver Ischemia/reperfusion Induced Apoptosis By Epigallocatechin-3-gallate Via Down-regulation of NF-kappaB and c-Jun Expression." The Journal of Surgical Research, vol. 159, no. 2, 2010, pp. 720-8.
Giakoustidis DE, Giakoustidis AE, Iliadis S, et al. Attenuation of liver ischemia/reperfusion induced apoptosis by epigallocatechin-3-gallate via down-regulation of NF-kappaB and c-Jun expression. J Surg Res. 2010;159(2):720-8.
Giakoustidis, D. E., Giakoustidis, A. E., Iliadis, S., Koliakou, K., Antoniadis, N., Kontos, N., Papanikolaou, V., Papageorgiou, G., Kaldrimidou, E., & Takoudas, D. (2010). Attenuation of liver ischemia/reperfusion induced apoptosis by epigallocatechin-3-gallate via down-regulation of NF-kappaB and c-Jun expression. The Journal of Surgical Research, 159(2), 720-8. https://doi.org/10.1016/j.jss.2008.08.038
Giakoustidis DE, et al. Attenuation of Liver Ischemia/reperfusion Induced Apoptosis By Epigallocatechin-3-gallate Via Down-regulation of NF-kappaB and c-Jun Expression. J Surg Res. 2010;159(2):720-8. PubMed PMID: 19394642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attenuation of liver ischemia/reperfusion induced apoptosis by epigallocatechin-3-gallate via down-regulation of NF-kappaB and c-Jun expression. AU - Giakoustidis,Dimitrios E, AU - Giakoustidis,Alexandros E, AU - Iliadis,Stavros, AU - Koliakou,Kokona, AU - Antoniadis,Nikolaos, AU - Kontos,Nikolaos, AU - Papanikolaou,Vasilios, AU - Papageorgiou,Georgios, AU - Kaldrimidou,Eleni, AU - Takoudas,Dimitrios, Y1 - 2008/09/29/ PY - 2008/06/27/received PY - 2008/08/19/revised PY - 2008/08/29/accepted PY - 2009/4/28/entrez PY - 2009/4/28/pubmed PY - 2010/4/24/medline SP - 720 EP - 8 JF - The Journal of surgical research JO - J Surg Res VL - 159 IS - 2 N2 - INTRODUCTION: Hepatic ischemia/reperfusion (I/R) activates Kupffer cells and initiates severe oxidative stress with enhanced production of reactive oxygen species (ROS) and tumor necrosis factor-alpha (TNF-alpha). ROS and TNF-alpha mediate the expression of nuclear factors and kinases, activating the signal transduction pathway, and triggering apoptosis. The aim of our study was to evaluate the potential protective effect of (-)-epigallocatechin-3-gallate (EGCG) administration in inhibition of apoptosis by attenuating the expression of NF-kappaB, c-Jun, and caspase-3 in a model of severe hepatic I/R. MATERIALS AND METHODS: Thirty Wistar rats were allocated into three groups. Sham operation, I/R, and I/R-EGCG 50mg/kg. Hepatic ischemia was induced for 60min by Pringle's maneuver. Malondialdehyde (MDA), myeloperoxidase (MPO), light histology, scanning electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and immunocytochemistry for NF-kappaB, c-Jun, caspase-3, analysis on liver specimens and aspartate (AST), and alanine (ALT) transferases analysis in serum, were performed 120min after reperfusion. RESULTS: Apoptosis as indicated by TUNEL and caspase-3 was widely expressed in the I/R group but very limited in the EGCG treated group. Liver was stained positive for NF-kappaB and c-Jun in the I/R group but failed to be stained positive in the EGCG treated group. MDA, MPO, AST, and ALT showed marked increase in the I/R group and significant decrease in EGCG treated group. Significant alterations of liver specimens were observed by light histology and transmission electron microscopy whilst pretreatment with EGCG resulted in parenchymal preservation. CONCLUSIONS: Administration of EGCG is likely to inhibit I/R-induced apoptosis and protect liver by down-regulating NF-kappaB and c-Jun signal transduction pathways. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/19394642/Attenuation_of_liver_ischemia/reperfusion_induced_apoptosis_by_epigallocatechin_3_gallate_via_down_regulation_of_NF_kappaB_and_c_Jun_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(08)00582-9 DB - PRIME DP - Unbound Medicine ER -