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Application of silver ion in the separation of macrolide antibiotic components by high-speed counter-current chromatography.
J Chromatogr A. 2009 May 29; 1216(22):4668-72.JC

Abstract

Three macrolide antibiotic components - ascomycin, tacrolimus and dihydrotacrolimus - were separated and purified by silver ion high-speed counter-current chromatography (HSCCC). The solvent system consisted of n-hexane-tert-butyl methyl ether-methanol-water (1:3:6:5, v/v) and silver nitrate (0.10mol/l). The silver ion acted as a pi-complexing agent with tacrolimus because of its extra side double bond compared with ascomycin and dihydrotacrolimus. This complexation modified the partition coefficient values and the separation factors of the three components. As a result, ascomycin, tacrolimus and dihydrotacrolimus were purified from 150mg extracted crude sample with purities of 97.6%, 98.7% and 96.5%, respectively, and yields over 80% (including their tautomers). These results cannot be achieved with the same solvent system but without the addition of silver ion.

Authors+Show Affiliations

Fujian Institute of Microbiology, Fuzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19394946

Citation

Wen, Yaoming, et al. "Application of Silver Ion in the Separation of Macrolide Antibiotic Components By High-speed Counter-current Chromatography." Journal of Chromatography. A, vol. 1216, no. 22, 2009, pp. 4668-72.
Wen Y, Wang J, Chen X, et al. Application of silver ion in the separation of macrolide antibiotic components by high-speed counter-current chromatography. J Chromatogr A. 2009;1216(22):4668-72.
Wen, Y., Wang, J., Chen, X., Le, Z., Chen, Y., & Zheng, W. (2009). Application of silver ion in the separation of macrolide antibiotic components by high-speed counter-current chromatography. Journal of Chromatography. A, 1216(22), 4668-72. https://doi.org/10.1016/j.chroma.2009.03.082
Wen Y, et al. Application of Silver Ion in the Separation of Macrolide Antibiotic Components By High-speed Counter-current Chromatography. J Chromatogr A. 2009 May 29;1216(22):4668-72. PubMed PMID: 19394946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of silver ion in the separation of macrolide antibiotic components by high-speed counter-current chromatography. AU - Wen,Yaoming, AU - Wang,Jiaoyan, AU - Chen,Xiuming, AU - Le,Zhanxian, AU - Chen,Yuxiang, AU - Zheng,Wei, Y1 - 2009/04/07/ PY - 2008/12/23/received PY - 2009/03/24/revised PY - 2009/03/30/accepted PY - 2009/4/28/entrez PY - 2009/4/28/pubmed PY - 2009/7/2/medline SP - 4668 EP - 72 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 22 N2 - Three macrolide antibiotic components - ascomycin, tacrolimus and dihydrotacrolimus - were separated and purified by silver ion high-speed counter-current chromatography (HSCCC). The solvent system consisted of n-hexane-tert-butyl methyl ether-methanol-water (1:3:6:5, v/v) and silver nitrate (0.10mol/l). The silver ion acted as a pi-complexing agent with tacrolimus because of its extra side double bond compared with ascomycin and dihydrotacrolimus. This complexation modified the partition coefficient values and the separation factors of the three components. As a result, ascomycin, tacrolimus and dihydrotacrolimus were purified from 150mg extracted crude sample with purities of 97.6%, 98.7% and 96.5%, respectively, and yields over 80% (including their tautomers). These results cannot be achieved with the same solvent system but without the addition of silver ion. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19394946/Application_of_silver_ion_in_the_separation_of_macrolide_antibiotic_components_by_high_speed_counter_current_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00516-0 DB - PRIME DP - Unbound Medicine ER -