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Continuous ethanol production from cassava through simultaneous saccharification and fermentation by self-flocculating yeast Saccharomyces cerevisiae CHFY0321.
Appl Biochem Biotechnol. 2010 Mar; 160(5):1517-27.AB

Abstract

In this study, a fermentor consisting of four linked stirred towers that can be used for simultaneous saccharification and fermentation (SSF) and for the accumulation of cell mass was applied to the continuous production of ethanol using cassava as the starchy material. For the continuous process with SSF, the pretreated cassava liquor and saccharification enzyme at total sugar concentrations of 175 g/L and 195 g/L were continuously fed to the fermentor with dilution rates of 0.014, 0.021, 0.031, 0.042, and 0.05 h(-1). Considering the maximum saccharification time, the highest volumetric productivity and ethanol yield were observed at a dilution rate of 0.042 h(-1). At dilution rates in the range of 0.014 h(-1) to 0.042 h(-1), high production rates were observed, and the yeast in the first to fourth fermentor showed long-term stability for 2 months with good performance. Under the optimal culture conditions with a feed sugar concentration of 195 g/L and dilution rate of 0.042 h(-1), the ethanol volumetric productivity and ethanol yield were 3.58 g/L x h and 86.2%, respectively. The cell concentrations in the first to fourth stirred tower fermentors were 74.3, 71.5, 71.2, and 70.1 g dry cell/L, respectively. The self-flocculating yeast, Saccharomyces cerevisiae CHFY0321, developed by our group showed excellent fermentation results under continuous ethanol production.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Choi GW
Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd., Palbok-Dong 829, Dukjin-Gu, Jeonju 561-203, Korea. changrd@chethanol.com
Kang HW
No affiliation info available
Moon SK
No affiliation info available
Chung BW
No affiliation info available

MeSH

BiomassBioreactorsBiotechnologyCarbohydratesEthanolFermentationFlocculationManihotSaccharomyces cerevisiae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19396636

Citation

Choi, Gi-Wook, et al. "Continuous Ethanol Production From Cassava Through Simultaneous Saccharification and Fermentation By Self-flocculating Yeast Saccharomyces Cerevisiae CHFY0321." Applied Biochemistry and Biotechnology, vol. 160, no. 5, 2010, pp. 1517-27.
Choi GW, Kang HW, Moon SK, et al. Continuous ethanol production from cassava through simultaneous saccharification and fermentation by self-flocculating yeast Saccharomyces cerevisiae CHFY0321. Appl Biochem Biotechnol. 2010;160(5):1517-27.
Choi, G. W., Kang, H. W., Moon, S. K., & Chung, B. W. (2010). Continuous ethanol production from cassava through simultaneous saccharification and fermentation by self-flocculating yeast Saccharomyces cerevisiae CHFY0321. Applied Biochemistry and Biotechnology, 160(5), 1517-27. https://doi.org/10.1007/s12010-009-8653-5
Choi GW, et al. Continuous Ethanol Production From Cassava Through Simultaneous Saccharification and Fermentation By Self-flocculating Yeast Saccharomyces Cerevisiae CHFY0321. Appl Biochem Biotechnol. 2010;160(5):1517-27. PubMed PMID: 19396636.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous ethanol production from cassava through simultaneous saccharification and fermentation by self-flocculating yeast Saccharomyces cerevisiae CHFY0321. AU - Choi,Gi-Wook, AU - Kang,Hyun-Woo, AU - Moon,Se-Kwon, AU - Chung,Bong-Woo, Y1 - 2009/04/28/ PY - 2009/01/23/received PY - 2009/04/14/accepted PY - 2009/4/28/entrez PY - 2009/4/28/pubmed PY - 2010/5/4/medline SP - 1517 EP - 27 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 160 IS - 5 N2 - In this study, a fermentor consisting of four linked stirred towers that can be used for simultaneous saccharification and fermentation (SSF) and for the accumulation of cell mass was applied to the continuous production of ethanol using cassava as the starchy material. For the continuous process with SSF, the pretreated cassava liquor and saccharification enzyme at total sugar concentrations of 175 g/L and 195 g/L were continuously fed to the fermentor with dilution rates of 0.014, 0.021, 0.031, 0.042, and 0.05 h(-1). Considering the maximum saccharification time, the highest volumetric productivity and ethanol yield were observed at a dilution rate of 0.042 h(-1). At dilution rates in the range of 0.014 h(-1) to 0.042 h(-1), high production rates were observed, and the yeast in the first to fourth fermentor showed long-term stability for 2 months with good performance. Under the optimal culture conditions with a feed sugar concentration of 195 g/L and dilution rate of 0.042 h(-1), the ethanol volumetric productivity and ethanol yield were 3.58 g/L x h and 86.2%, respectively. The cell concentrations in the first to fourth stirred tower fermentors were 74.3, 71.5, 71.2, and 70.1 g dry cell/L, respectively. The self-flocculating yeast, Saccharomyces cerevisiae CHFY0321, developed by our group showed excellent fermentation results under continuous ethanol production. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/19396636/Continuous_ethanol_production_from_cassava_through_simultaneous_saccharification_and_fermentation_by_self_flocculating_yeast_Saccharomyces_cerevisiae_CHFY0321_ L2 - https://dx.doi.org/10.1007/s12010-009-8653-5 DB - PRIME DP - Unbound Medicine ER -