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Mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents.
J Ethnopharmacol. 2009 Jul 15; 124(2):233-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Phyllanthus niruri Linn. (Euphorbiaceae) is used as folk medicine in South America to treat excess uric acid. Our initial study showed that the methanol extract of Phyllanthus niruri and its lignans were able to reverse the plasma uric acid of hyperuricemic animals.

AIM OF THE STUDY

The study was undertaken to investigate the mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents.

MATERIAL AND METHODS

The mechanisms were investigated using xanthine oxidase assay and uricosuric studies in potassium oxonate- and uric acid-induced hyperuricemic rats.

RESULTS

Phyllanthus niruri methanol extract exhibited in vitro xanthine oxidase inhibition with an IC50 of 39.39 microg/mL and a moderate in vivo xanthine oxidase inhibitory activity. However, the lignans display poor xanthine oxidase inhibition in vitro and a relatively weak in vivo inhibitory activity at 10mg/kg. On the other hand, intraperitoneal treatment with Phyllanthus niruri methanol extract showed 1.69 folds increase in urinary uric acid excretion when compared to the hyperuricemic control animals. Likewise, the lignans, phyllanthin, hypophyllanthin and phyltetralin exhibited up to 2.51 and 11.0 folds higher in urinary uric acid excretion and clearance, respectively. The co-administration of pyrazinamide with phyllanthin exhibited a significant suppression of phyllanthin's uricosuric activity resembling that of pyrazinamide with benzbromarone.

CONCLUSIONS

The present study showed that the antihyperuricemic effect of Phyllanthus niruri methanol extract may be mainly due to its uricosuric action and partly through xanthine oxidase inhibition, whereas the antihyperuricemic effect of the lignans was attributed to their uricosuric action.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19397979

Citation

Murugaiyah, Vikneswaran, and Kit-Lam Chan. "Mechanisms of Antihyperuricemic Effect of Phyllanthus Niruri and Its Lignan Constituents." Journal of Ethnopharmacology, vol. 124, no. 2, 2009, pp. 233-9.
Murugaiyah V, Chan KL. Mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents. J Ethnopharmacol. 2009;124(2):233-9.
Murugaiyah, V., & Chan, K. L. (2009). Mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents. Journal of Ethnopharmacology, 124(2), 233-9. https://doi.org/10.1016/j.jep.2009.04.026
Murugaiyah V, Chan KL. Mechanisms of Antihyperuricemic Effect of Phyllanthus Niruri and Its Lignan Constituents. J Ethnopharmacol. 2009 Jul 15;124(2):233-9. PubMed PMID: 19397979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents. AU - Murugaiyah,Vikneswaran, AU - Chan,Kit-Lam, Y1 - 2009/05/03/ PY - 2008/12/03/received PY - 2009/04/03/revised PY - 2009/04/19/accepted PY - 2009/4/29/entrez PY - 2009/4/29/pubmed PY - 2009/10/13/medline SP - 233 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 124 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Phyllanthus niruri Linn. (Euphorbiaceae) is used as folk medicine in South America to treat excess uric acid. Our initial study showed that the methanol extract of Phyllanthus niruri and its lignans were able to reverse the plasma uric acid of hyperuricemic animals. AIM OF THE STUDY: The study was undertaken to investigate the mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents. MATERIAL AND METHODS: The mechanisms were investigated using xanthine oxidase assay and uricosuric studies in potassium oxonate- and uric acid-induced hyperuricemic rats. RESULTS: Phyllanthus niruri methanol extract exhibited in vitro xanthine oxidase inhibition with an IC50 of 39.39 microg/mL and a moderate in vivo xanthine oxidase inhibitory activity. However, the lignans display poor xanthine oxidase inhibition in vitro and a relatively weak in vivo inhibitory activity at 10mg/kg. On the other hand, intraperitoneal treatment with Phyllanthus niruri methanol extract showed 1.69 folds increase in urinary uric acid excretion when compared to the hyperuricemic control animals. Likewise, the lignans, phyllanthin, hypophyllanthin and phyltetralin exhibited up to 2.51 and 11.0 folds higher in urinary uric acid excretion and clearance, respectively. The co-administration of pyrazinamide with phyllanthin exhibited a significant suppression of phyllanthin's uricosuric activity resembling that of pyrazinamide with benzbromarone. CONCLUSIONS: The present study showed that the antihyperuricemic effect of Phyllanthus niruri methanol extract may be mainly due to its uricosuric action and partly through xanthine oxidase inhibition, whereas the antihyperuricemic effect of the lignans was attributed to their uricosuric action. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19397979/Mechanisms_of_antihyperuricemic_effect_of_Phyllanthus_niruri_and_its_lignan_constituents_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00262-1 DB - PRIME DP - Unbound Medicine ER -