Tags

Type your tag names separated by a space and hit enter

The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in Saccharomyces cerevisiae.
Yeast. 2009 Jun; 26(6):339-47.Y

Abstract

Saccharomyces cerevisiae lacks enzymes that contain the molybdopterin co-factor and therefore any requirement for molybdenum as a trace mineral supplement. Instead, high molybdate levels are inhibitory to its growth. Low cellular levels of heat shock protein 90 (Hsp90), an essential chaperone, were found to enhance this sensitivity to molybdate. Certain Hsp90 point mutations and co-chaperone protein defects that partially compromise the function of the Hsp90/Cdc37p chaperone system also rendered S. cerevisiae hypersensitive to high molybdate levels. Sensitivity was especially apparent with mutations close to the Hsp90 nucleotide binding site, with the loss of the non-essential co-chaperone Sti1p (the equivalent of mammalian Hop), and with the abolition of residue Ser14 phosphorylation on the essential co-chaperone Cdc37p. While it remains to be proved that these effects reflect direct inhibition of the Hsp90 of the cell by the MoO(4) (2+) oxyanion in vivo; this possibility is suggested by molybdate sensitivity arising with a mutation in the Hsp90 nucleotide binding site that does not generate stress sensitivity or an impaired stress response. Molybdate sensitivity may therefore be a useful phenotype to score when studying mutations in this chaperone system.

Authors+Show Affiliations

Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19399909

Citation

Millson, Stefan H., et al. "The Hsp90/Cdc37p Chaperone System Is a Determinant of Molybdate Resistance in Saccharomyces Cerevisiae." Yeast (Chichester, England), vol. 26, no. 6, 2009, pp. 339-47.
Millson SH, Nuttall JM, Mollapour M, et al. The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in Saccharomyces cerevisiae. Yeast. 2009;26(6):339-47.
Millson, S. H., Nuttall, J. M., Mollapour, M., & Piper, P. W. (2009). The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in Saccharomyces cerevisiae. Yeast (Chichester, England), 26(6), 339-47. https://doi.org/10.1002/yea.1670
Millson SH, et al. The Hsp90/Cdc37p Chaperone System Is a Determinant of Molybdate Resistance in Saccharomyces Cerevisiae. Yeast. 2009;26(6):339-47. PubMed PMID: 19399909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in Saccharomyces cerevisiae. AU - Millson,Stefan H, AU - Nuttall,James M, AU - Mollapour,Mehdi, AU - Piper,Peter W, PY - 2009/4/29/entrez PY - 2009/4/29/pubmed PY - 2009/7/10/medline SP - 339 EP - 47 JF - Yeast (Chichester, England) JO - Yeast VL - 26 IS - 6 N2 - Saccharomyces cerevisiae lacks enzymes that contain the molybdopterin co-factor and therefore any requirement for molybdenum as a trace mineral supplement. Instead, high molybdate levels are inhibitory to its growth. Low cellular levels of heat shock protein 90 (Hsp90), an essential chaperone, were found to enhance this sensitivity to molybdate. Certain Hsp90 point mutations and co-chaperone protein defects that partially compromise the function of the Hsp90/Cdc37p chaperone system also rendered S. cerevisiae hypersensitive to high molybdate levels. Sensitivity was especially apparent with mutations close to the Hsp90 nucleotide binding site, with the loss of the non-essential co-chaperone Sti1p (the equivalent of mammalian Hop), and with the abolition of residue Ser14 phosphorylation on the essential co-chaperone Cdc37p. While it remains to be proved that these effects reflect direct inhibition of the Hsp90 of the cell by the MoO(4) (2+) oxyanion in vivo; this possibility is suggested by molybdate sensitivity arising with a mutation in the Hsp90 nucleotide binding site that does not generate stress sensitivity or an impaired stress response. Molybdate sensitivity may therefore be a useful phenotype to score when studying mutations in this chaperone system. SN - 1097-0061 UR - https://www.unboundmedicine.com/medline/citation/19399909/The_Hsp90/Cdc37p_chaperone_system_is_a_determinant_of_molybdate_resistance_in_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1002/yea.1670 DB - PRIME DP - Unbound Medicine ER -