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Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model.
World J Gastroenterol. 2009 Apr 28; 15(16):1977-84.WJ

Abstract

AIM

To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium, and zinc bioavailability by estimating the uptake, retention, and transport by Caco-2 cells.

METHODS

Both dephytinized (by adding an exogenous phytase) and non-dephytinized infant cereals were digested using an in vitro digestion protocol adapted to the gastrointestinal conditions of infants younger than 6 mo. Mineral cell retention, transport, and uptake from infant cereals were measured using the soluble fraction of the simulated digestion and the Caco-2 cells.

RESULTS

Dephytinization of infant cereals significantly increased (P < 0.05) the cell uptake efficiency (from 0.66%-6.05% to 3.93%-13%), retention (from 6.04%-16.68% to 14.75%-20.14%) and transport efficiency (from 0.14%-2.21% to 1.47%-6.02%), of iron, and the uptake efficiency (from 5.0%-35.4% to 7.3%-41.6%) and retention (from 4.05%-20.53% to 14.45%-61.3%) of zinc, whereas calcium only cell uptake showed a significant increase (P < 0.05) after removing phytate from most of the samples analyzed. A positive relationship (P < 0.05) between mineral solubility and the cell uptake and transport efficiencies was observed.

CONCLUSION

Removing phytate from infant cereals had a beneficial effect on iron and zinc bioavailability when infant cereals were reconstituted with water. Since in developing countries cereal-based complementary foods for infants are usually consumed mixed with water, exogenous phytase additions could improve the nutritional value of this weaning food.

Authors+Show Affiliations

Department of Food Science and Nutrition, Faculty of Veterinary Science and Food Science and Technology, Murcia University, Murcia, Spain. carmenfr@um.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19399930

Citation

Frontela, Carmen, et al. "Effect of Dephytinization On Bioavailability of Iron, Calcium and Zinc From Infant Cereals Assessed in the Caco-2 Cell Model." World Journal of Gastroenterology, vol. 15, no. 16, 2009, pp. 1977-84.
Frontela C, Scarino ML, Ferruzza S, et al. Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model. World J Gastroenterol. 2009;15(16):1977-84.
Frontela, C., Scarino, M. L., Ferruzza, S., Ros, G., & Martínez, C. (2009). Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model. World Journal of Gastroenterology, 15(16), 1977-84.
Frontela C, et al. Effect of Dephytinization On Bioavailability of Iron, Calcium and Zinc From Infant Cereals Assessed in the Caco-2 Cell Model. World J Gastroenterol. 2009 Apr 28;15(16):1977-84. PubMed PMID: 19399930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model. AU - Frontela,Carmen, AU - Scarino,Maria-Laura, AU - Ferruzza,Simonetta, AU - Ros,Gaspar, AU - Martínez,Carmen, PY - 2009/4/29/entrez PY - 2009/4/29/pubmed PY - 2009/5/27/medline SP - 1977 EP - 84 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 15 IS - 16 N2 - AIM: To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium, and zinc bioavailability by estimating the uptake, retention, and transport by Caco-2 cells. METHODS: Both dephytinized (by adding an exogenous phytase) and non-dephytinized infant cereals were digested using an in vitro digestion protocol adapted to the gastrointestinal conditions of infants younger than 6 mo. Mineral cell retention, transport, and uptake from infant cereals were measured using the soluble fraction of the simulated digestion and the Caco-2 cells. RESULTS: Dephytinization of infant cereals significantly increased (P < 0.05) the cell uptake efficiency (from 0.66%-6.05% to 3.93%-13%), retention (from 6.04%-16.68% to 14.75%-20.14%) and transport efficiency (from 0.14%-2.21% to 1.47%-6.02%), of iron, and the uptake efficiency (from 5.0%-35.4% to 7.3%-41.6%) and retention (from 4.05%-20.53% to 14.45%-61.3%) of zinc, whereas calcium only cell uptake showed a significant increase (P < 0.05) after removing phytate from most of the samples analyzed. A positive relationship (P < 0.05) between mineral solubility and the cell uptake and transport efficiencies was observed. CONCLUSION: Removing phytate from infant cereals had a beneficial effect on iron and zinc bioavailability when infant cereals were reconstituted with water. Since in developing countries cereal-based complementary foods for infants are usually consumed mixed with water, exogenous phytase additions could improve the nutritional value of this weaning food. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/19399930/Effect_of_dephytinization_on_bioavailability_of_iron_calcium_and_zinc_from_infant_cereals_assessed_in_the_Caco_2_cell_model_ L2 - https://www.wjgnet.com/1007-9327/full/v15/i16/1977.htm DB - PRIME DP - Unbound Medicine ER -