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SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice.
Brain Res 2009; 1261:100-8BR

Abstract

SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Abeta(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Abeta(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Abeta antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H(2)O(2) in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

Authors+Show Affiliations

Department of Chemistry and Nano Science, and Brain Disease Research Institute, Ewha Womans University, Seodaemoon-Gu, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19401163

Citation

Seo, Ji-Seon, et al. "SK-PC-B70M Confers Anti-oxidant Activity and Reduces Abeta Levels in the Brain of Tg2576 Mice." Brain Research, vol. 1261, 2009, pp. 100-8.
Seo JS, Kim TK, Leem YH, et al. SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice. Brain Res. 2009;1261:100-8.
Seo, J. S., Kim, T. K., Leem, Y. H., Lee, K. W., Park, S. K., Baek, I. S., ... Han, P. L. (2009). SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice. Brain Research, 1261, pp. 100-8. doi:10.1016/j.brainres.2009.01.019.
Seo JS, et al. SK-PC-B70M Confers Anti-oxidant Activity and Reduces Abeta Levels in the Brain of Tg2576 Mice. Brain Res. 2009 Mar 19;1261:100-8. PubMed PMID: 19401163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice. AU - Seo,Ji-Seon, AU - Kim,Tae-Kyung, AU - Leem,Yea-Hyun, AU - Lee,Kang-Woo, AU - Park,Sun-Kyu, AU - Baek,In-Sun, AU - Kim,Kyoung-Shim, AU - Im,Guang-Jin, AU - Lee,Soo Min, AU - Park,Yang Hae, AU - Han,Pyung-Lim, Y1 - 2009/01/21/ PY - 2008/10/29/received PY - 2009/01/11/revised PY - 2009/01/12/accepted PY - 2009/4/30/entrez PY - 2009/4/30/pubmed PY - 2010/1/15/medline SP - 100 EP - 8 JF - Brain research JO - Brain Res. VL - 1261 N2 - SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Abeta(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Abeta(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Abeta antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H(2)O(2) in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/19401163/SK_PC_B70M_confers_anti_oxidant_activity_and_reduces_Abeta_levels_in_the_brain_of_Tg2576_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(09)00091-2 DB - PRIME DP - Unbound Medicine ER -