Tags

Type your tag names separated by a space and hit enter

A comparative study of the effects of genistein, estradiol and raloxifene on the murine skeletal system.
Acta Biochim Pol. 2009; 56(2):261-70.AB

Abstract

Genistein, a major phytoestrogen of soy, is considered a potential drug for prevention and treatment of postmenopausal osteoporosis. The aim of the present study was to compare the effects of genistein, estradiol and raloxifene on the skeletal system in vivo and in vitro. Genistein (5 mg/kg), estradiol (0.1 mg/kg) or raloxifene hydrochloride (5 mg/kg) were administered daily by a stomach tube to mature ovariectomized Wistar rats for 4 weeks. Bone mass, mineral and calcium content, macrometric parameters and mechanical properties were examined. Also the effects of genistein, estradiol and raloxifene (10(-9)-10(-7) M) on the formation of osteoclasts from neonatal mouse bone marrow cells and the activity of osteoblasts isolated from neonatal mouse calvariae were compared. In vivo, estrogen deficiency resulted in the impairment of bone mineralization and bone mechanical properties. Raloxifene but not estradiol or genistein improved bone mineralization. Estradiol fully normalized the bone mechanical properties, whereas genistein augmented the deleterious effect of estrogen-deficiency on bone strength. In vitro, genistein, estradiol and raloxifene inhibited osteoclast formation from mouse bone marrow cells, decreasing the ratio of RANKL mRNA to osteoprotegerin mRNA expression in osteoblasts. Genistein, but not estradiol or raloxifene, decreased the ratio of alkaline phosphatase mRNA to ectonucleotide pyrophosphatase phosphodiesterase 1 mRNA expression in osteoblasts. This difference may explain the lack of genistein effect on bone mineralization observed in ovariectomized rats in the in vivo study. Concluding, our experiments demonstrated profound differences between the activities of genistein, estradiol and raloxifene towards the osseous tissue in experimental conditions.

Authors+Show Affiliations

Department of Pharmacology, Medical University of Silesia, Sosnowiec, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19401787

Citation

Sliwiński, Leszek, et al. "A Comparative Study of the Effects of Genistein, Estradiol and Raloxifene On the Murine Skeletal System." Acta Biochimica Polonica, vol. 56, no. 2, 2009, pp. 261-70.
Sliwiński L, Folwarczna J, Nowińska B, et al. A comparative study of the effects of genistein, estradiol and raloxifene on the murine skeletal system. Acta Biochim Pol. 2009;56(2):261-70.
Sliwiński, L., Folwarczna, J., Nowińska, B., Cegieła, U., Pytlik, M., Kaczmarczyk-Sedlak, I., Trzeciak, H., & Trzeciak, H. I. (2009). A comparative study of the effects of genistein, estradiol and raloxifene on the murine skeletal system. Acta Biochimica Polonica, 56(2), 261-70.
Sliwiński L, et al. A Comparative Study of the Effects of Genistein, Estradiol and Raloxifene On the Murine Skeletal System. Acta Biochim Pol. 2009;56(2):261-70. PubMed PMID: 19401787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study of the effects of genistein, estradiol and raloxifene on the murine skeletal system. AU - Sliwiński,Leszek, AU - Folwarczna,Joanna, AU - Nowińska,Barbara, AU - Cegieła,Urszula, AU - Pytlik,Maria, AU - Kaczmarczyk-Sedlak,Ilona, AU - Trzeciak,Hanna, AU - Trzeciak,Henryk I, Y1 - 2009/04/30/ PY - 2009/01/20/received PY - 2009/03/26/revised PY - 2009/04/24/accepted PY - 2009/4/30/entrez PY - 2009/4/30/pubmed PY - 2009/10/15/medline SP - 261 EP - 70 JF - Acta biochimica Polonica JO - Acta Biochim Pol VL - 56 IS - 2 N2 - Genistein, a major phytoestrogen of soy, is considered a potential drug for prevention and treatment of postmenopausal osteoporosis. The aim of the present study was to compare the effects of genistein, estradiol and raloxifene on the skeletal system in vivo and in vitro. Genistein (5 mg/kg), estradiol (0.1 mg/kg) or raloxifene hydrochloride (5 mg/kg) were administered daily by a stomach tube to mature ovariectomized Wistar rats for 4 weeks. Bone mass, mineral and calcium content, macrometric parameters and mechanical properties were examined. Also the effects of genistein, estradiol and raloxifene (10(-9)-10(-7) M) on the formation of osteoclasts from neonatal mouse bone marrow cells and the activity of osteoblasts isolated from neonatal mouse calvariae were compared. In vivo, estrogen deficiency resulted in the impairment of bone mineralization and bone mechanical properties. Raloxifene but not estradiol or genistein improved bone mineralization. Estradiol fully normalized the bone mechanical properties, whereas genistein augmented the deleterious effect of estrogen-deficiency on bone strength. In vitro, genistein, estradiol and raloxifene inhibited osteoclast formation from mouse bone marrow cells, decreasing the ratio of RANKL mRNA to osteoprotegerin mRNA expression in osteoblasts. Genistein, but not estradiol or raloxifene, decreased the ratio of alkaline phosphatase mRNA to ectonucleotide pyrophosphatase phosphodiesterase 1 mRNA expression in osteoblasts. This difference may explain the lack of genistein effect on bone mineralization observed in ovariectomized rats in the in vivo study. Concluding, our experiments demonstrated profound differences between the activities of genistein, estradiol and raloxifene towards the osseous tissue in experimental conditions. SN - 1734-154X UR - https://www.unboundmedicine.com/medline/citation/19401787/A_comparative_study_of_the_effects_of_genistein_estradiol_and_raloxifene_on_the_murine_skeletal_system_ L2 - http://www.actabp.pl/pdf/2_2009/261.pdf DB - PRIME DP - Unbound Medicine ER -