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Comparison of the phenolic component profiles of skullcap (Scutellaria lateriflora) and germander (Teucrium canadense and T. chamaedrys), a potentially hepatotoxic adulterant.
Phytochem Anal. 2009 Jul-Aug; 20(4):298-306.PA

Abstract

INTRODUCTION

Scutellaria lateriflora, commonly known as skullcap, is used as an ingredient in numerous herbal products. Unfortunately, it has occasionally been adulterated with Teucrium canadense or T. chamaedrys, commonly known as germander, which contains potentially hepatotoxic diterpenes. Chromatographic profiles of the phenolic components provide a means of distinguishing between these plants and enhancing public safety.

OBJECTIVE

To develop a chromatographic method for the identification of Scutellaria lateriflora and two Teucrium species and to quantify the latter as adulterants.

METHODOLOGY

Samples were extracted with aqueous methanol and the extracts were analysed using a standardised LC-DAD-ESI/MS profiling method to obtain their phenolic profiles.

RESULTS

Skullcap contained primarily flavonoids, while the major phenolic components of the two Teucrium species were the phenylethanoids, verbascoside and teucrioside. Using the phenylethanoids as markers, it was possible to clearly distinguish between the two genus and to determine 5% Teucrium mixed with Scutellaria using either ultraviolet absorption spectrometry or mass spectrometry in the total ion count mode. Using MS in the selective ion monitoring (SIM) mode, 1% Teucrium could be measured.

CONCLUSIONS

This study showed that chromatographic profiling was able to identify Scutellaria and Teucrium, separately and when mixed together.

Authors+Show Affiliations

Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, 103000 Baltimore Avenue, Building-161, BARC-East, Beltsville, MD 20705, USA. longze.lin@ ars.usda.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19402188

Citation

Lin, Long-Ze, et al. "Comparison of the Phenolic Component Profiles of Skullcap (Scutellaria Lateriflora) and Germander (Teucrium Canadense and T. Chamaedrys), a Potentially Hepatotoxic Adulterant." Phytochemical Analysis : PCA, vol. 20, no. 4, 2009, pp. 298-306.
Lin LZ, Harnly JM, Upton R. Comparison of the phenolic component profiles of skullcap (Scutellaria lateriflora) and germander (Teucrium canadense and T. chamaedrys), a potentially hepatotoxic adulterant. Phytochem Anal. 2009;20(4):298-306.
Lin, L. Z., Harnly, J. M., & Upton, R. (2009). Comparison of the phenolic component profiles of skullcap (Scutellaria lateriflora) and germander (Teucrium canadense and T. chamaedrys), a potentially hepatotoxic adulterant. Phytochemical Analysis : PCA, 20(4), 298-306. https://doi.org/10.1002/pca.1127
Lin LZ, Harnly JM, Upton R. Comparison of the Phenolic Component Profiles of Skullcap (Scutellaria Lateriflora) and Germander (Teucrium Canadense and T. Chamaedrys), a Potentially Hepatotoxic Adulterant. Phytochem Anal. 2009 Jul-Aug;20(4):298-306. PubMed PMID: 19402188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the phenolic component profiles of skullcap (Scutellaria lateriflora) and germander (Teucrium canadense and T. chamaedrys), a potentially hepatotoxic adulterant. AU - Lin,Long-Ze, AU - Harnly,James M, AU - Upton,Roy, PY - 2009/4/30/entrez PY - 2009/4/30/pubmed PY - 2009/7/25/medline SP - 298 EP - 306 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 20 IS - 4 N2 - INTRODUCTION: Scutellaria lateriflora, commonly known as skullcap, is used as an ingredient in numerous herbal products. Unfortunately, it has occasionally been adulterated with Teucrium canadense or T. chamaedrys, commonly known as germander, which contains potentially hepatotoxic diterpenes. Chromatographic profiles of the phenolic components provide a means of distinguishing between these plants and enhancing public safety. OBJECTIVE: To develop a chromatographic method for the identification of Scutellaria lateriflora and two Teucrium species and to quantify the latter as adulterants. METHODOLOGY: Samples were extracted with aqueous methanol and the extracts were analysed using a standardised LC-DAD-ESI/MS profiling method to obtain their phenolic profiles. RESULTS: Skullcap contained primarily flavonoids, while the major phenolic components of the two Teucrium species were the phenylethanoids, verbascoside and teucrioside. Using the phenylethanoids as markers, it was possible to clearly distinguish between the two genus and to determine 5% Teucrium mixed with Scutellaria using either ultraviolet absorption spectrometry or mass spectrometry in the total ion count mode. Using MS in the selective ion monitoring (SIM) mode, 1% Teucrium could be measured. CONCLUSIONS: This study showed that chromatographic profiling was able to identify Scutellaria and Teucrium, separately and when mixed together. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/19402188/Comparison_of_the_phenolic_component_profiles_of_skullcap__Scutellaria_lateriflora__and_germander__Teucrium_canadense_and_T__chamaedrys__a_potentially_hepatotoxic_adulterant_ L2 - https://doi.org/10.1002/pca.1127 DB - PRIME DP - Unbound Medicine ER -