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The polyadenylation site of Mimivirus transcripts obeys a stringent 'hairpin rule'.
Genome Res. 2009 Jul; 19(7):1233-42.GR

Abstract

Mimivirus, a giant DNA virus infecting Acanthamoeba, is revealing an increasing list of unique features such as a 1.2-Mb genome with numerous genes not found in other viruses, a uniquely conserved promoter signal, and a particle of unmatched complexity using two distinct portals for genome delivery and packaging. Herein, we contribute a further Mimivirus distinctive feature discovered by sequencing a panel of viral cDNAs produced for probing the structure of Mimivirus transcripts. All Mimivirus mRNAs are polyadenylated at a site coinciding exactly with unrelated, but strongly palindromic, genomic sequences. The analysis of 454 Life Sciences (Roche) FLX cDNA tags (150,651) confirmed this finding for all Mimivirus genes independent of their transcription timings and expression levels. The absence of a suitable palindromic signal between adjacent genes results in transcripts encompassing multiple ORFs in the same or even in opposite orientations. Surprisingly, Mimivirus tRNAs are expressed as polyadenylated messengers, including an ORF/tRNA composite mRNA. To our knowledge, both the nature and the stringency of the "hairpin rule" defining the location of polyadenylation sites are unique, raising once more the question of Mimivirus's evolutionary origin. The precise molecular mechanisms implementing the hairpin rule into the 3'-end processing of Mimivirus pre-mRNAs remain to be elucidated.

Authors+Show Affiliations

Structural and Genomic Information Laboratory, CNRS-UPR 2589, IFR-88, Aix-Marseille University, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19403753

Citation

Byrne, Deborah, et al. "The Polyadenylation Site of Mimivirus Transcripts Obeys a Stringent 'hairpin Rule'." Genome Research, vol. 19, no. 7, 2009, pp. 1233-42.
Byrne D, Grzela R, Lartigue A, et al. The polyadenylation site of Mimivirus transcripts obeys a stringent 'hairpin rule'. Genome Res. 2009;19(7):1233-42.
Byrne, D., Grzela, R., Lartigue, A., Audic, S., Chenivesse, S., Encinas, S., Claverie, J. M., & Abergel, C. (2009). The polyadenylation site of Mimivirus transcripts obeys a stringent 'hairpin rule'. Genome Research, 19(7), 1233-42. https://doi.org/10.1101/gr.091561.109
Byrne D, et al. The Polyadenylation Site of Mimivirus Transcripts Obeys a Stringent 'hairpin Rule'. Genome Res. 2009;19(7):1233-42. PubMed PMID: 19403753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The polyadenylation site of Mimivirus transcripts obeys a stringent 'hairpin rule'. AU - Byrne,Deborah, AU - Grzela,Renata, AU - Lartigue,Audrey, AU - Audic,Stéphane, AU - Chenivesse,Sabine, AU - Encinas,Stéphanie, AU - Claverie,Jean-Michel, AU - Abergel,Chantal, Y1 - 2009/04/29/ PY - 2009/5/1/entrez PY - 2009/5/1/pubmed PY - 2009/9/5/medline SP - 1233 EP - 42 JF - Genome research JO - Genome Res VL - 19 IS - 7 N2 - Mimivirus, a giant DNA virus infecting Acanthamoeba, is revealing an increasing list of unique features such as a 1.2-Mb genome with numerous genes not found in other viruses, a uniquely conserved promoter signal, and a particle of unmatched complexity using two distinct portals for genome delivery and packaging. Herein, we contribute a further Mimivirus distinctive feature discovered by sequencing a panel of viral cDNAs produced for probing the structure of Mimivirus transcripts. All Mimivirus mRNAs are polyadenylated at a site coinciding exactly with unrelated, but strongly palindromic, genomic sequences. The analysis of 454 Life Sciences (Roche) FLX cDNA tags (150,651) confirmed this finding for all Mimivirus genes independent of their transcription timings and expression levels. The absence of a suitable palindromic signal between adjacent genes results in transcripts encompassing multiple ORFs in the same or even in opposite orientations. Surprisingly, Mimivirus tRNAs are expressed as polyadenylated messengers, including an ORF/tRNA composite mRNA. To our knowledge, both the nature and the stringency of the "hairpin rule" defining the location of polyadenylation sites are unique, raising once more the question of Mimivirus's evolutionary origin. The precise molecular mechanisms implementing the hairpin rule into the 3'-end processing of Mimivirus pre-mRNAs remain to be elucidated. SN - 1088-9051 UR - https://www.unboundmedicine.com/medline/citation/19403753/The_polyadenylation_site_of_Mimivirus_transcripts_obeys_a_stringent_'hairpin_rule'_ L2 - http://genome.cshlp.org/cgi/pmidlookup?view=long&pmid=19403753 DB - PRIME DP - Unbound Medicine ER -