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Direct metabolic fingerprinting of commercial herbal tinctures by nuclear magnetic resonance spectroscopy and mass spectrometry.
Phytochem Anal. 2009 Jul-Aug; 20(4):328-34.PA

Abstract

INTRODUCTION

Tinctures are widely used liquid pharmaceutical preparations traditionally obtained by maceration of one or more medicinal plants in ethanol-water solutions. Such a process results in the extraction of virtually hundreds of structurally diverse compounds with different polarities. Owing to the large chemical diversity of the constituents present in the herbal tinctures, the analytical tools used for the quality control of tinctures are usually optimised only for the detection of single chemical entities or specific class of compounds.

OBJECTIVE

In order to overcome the major limitations of the current methods used for analysis of tinctures, a new methodological approach based on NMR spectroscopy and MS spectrometry has been tested with different commercial tinctures.

METHODOLOGY

Diffusion-edited 1H-NMR (1D DOSY) and 1H-NMR with suppression of the ethanol and water signals have been applied here for the first time to the direct analysis of commercial herbal tinctures derived from Echinacea purpurea, Hypericum perforatum, Ginkgo biloba and Valeriana officinalis. The direct injection of the tinctures in the MS detector in order to obtain the corresponding metabolic profiles was also performed.

RESULTS

Using both NMR and MS methods it was possible, without evaporation or separation steps, to obtain a metabolic fingerprint able to distinguish between tinctures prepared with different plants. Batch-to-batch homogeneity, as well as degradation after the expiry date of a batch, was also investigated.

CONCLUSION

The techniques proposed here represent fast and convenient direct analyses of medicinal herbal tinctures.

Authors+Show Affiliations

Universidade Catolica Portuguesa, Escola Superior de Biotecnologia, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. mpoliti@esb.ucp.ptNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19405164

Citation

Politi, Matteo, et al. "Direct Metabolic Fingerprinting of Commercial Herbal Tinctures By Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry." Phytochemical Analysis : PCA, vol. 20, no. 4, 2009, pp. 328-34.
Politi M, Zloh M, Pintado ME, et al. Direct metabolic fingerprinting of commercial herbal tinctures by nuclear magnetic resonance spectroscopy and mass spectrometry. Phytochem Anal. 2009;20(4):328-34.
Politi, M., Zloh, M., Pintado, M. E., Castro, P. M., Heinrich, M., & Prieto, J. M. (2009). Direct metabolic fingerprinting of commercial herbal tinctures by nuclear magnetic resonance spectroscopy and mass spectrometry. Phytochemical Analysis : PCA, 20(4), 328-34. https://doi.org/10.1002/pca.1131
Politi M, et al. Direct Metabolic Fingerprinting of Commercial Herbal Tinctures By Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry. Phytochem Anal. 2009 Jul-Aug;20(4):328-34. PubMed PMID: 19405164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct metabolic fingerprinting of commercial herbal tinctures by nuclear magnetic resonance spectroscopy and mass spectrometry. AU - Politi,Matteo, AU - Zloh,Mire, AU - Pintado,Manuela E, AU - Castro,Paula M L, AU - Heinrich,Michael, AU - Prieto,Jose M, PY - 2009/5/1/entrez PY - 2009/5/1/pubmed PY - 2009/7/25/medline SP - 328 EP - 34 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 20 IS - 4 N2 - INTRODUCTION: Tinctures are widely used liquid pharmaceutical preparations traditionally obtained by maceration of one or more medicinal plants in ethanol-water solutions. Such a process results in the extraction of virtually hundreds of structurally diverse compounds with different polarities. Owing to the large chemical diversity of the constituents present in the herbal tinctures, the analytical tools used for the quality control of tinctures are usually optimised only for the detection of single chemical entities or specific class of compounds. OBJECTIVE: In order to overcome the major limitations of the current methods used for analysis of tinctures, a new methodological approach based on NMR spectroscopy and MS spectrometry has been tested with different commercial tinctures. METHODOLOGY: Diffusion-edited 1H-NMR (1D DOSY) and 1H-NMR with suppression of the ethanol and water signals have been applied here for the first time to the direct analysis of commercial herbal tinctures derived from Echinacea purpurea, Hypericum perforatum, Ginkgo biloba and Valeriana officinalis. The direct injection of the tinctures in the MS detector in order to obtain the corresponding metabolic profiles was also performed. RESULTS: Using both NMR and MS methods it was possible, without evaporation or separation steps, to obtain a metabolic fingerprint able to distinguish between tinctures prepared with different plants. Batch-to-batch homogeneity, as well as degradation after the expiry date of a batch, was also investigated. CONCLUSION: The techniques proposed here represent fast and convenient direct analyses of medicinal herbal tinctures. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/19405164/Direct_metabolic_fingerprinting_of_commercial_herbal_tinctures_by_nuclear_magnetic_resonance_spectroscopy_and_mass_spectrometry_ L2 - https://doi.org/10.1002/pca.1131 DB - PRIME DP - Unbound Medicine ER -