Tags

Type your tag names separated by a space and hit enter

Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions.
Int J Syst Evol Microbiol. 2009 May; 59(Pt 5):987-91.IJ

Abstract

Bovine digital dermatitis (BDD) is a debilitating infection that is being increasingly recognized in livestock worldwide. Several treponeme phylotypes have been identified in BDD lesions, although only a single BDD-associated treponeme taxon (Treponema brennaborense) has been proposed thus far. In a previous study, we observed that four BDD-associated spirochaete isolates formed a distinct phylogenetic cluster on the basis of 16S rRNA gene sequence analysis and shared less than 97 % sequence similarity with recognized treponeme species. Further characterization of these isolates on the basis of growth characteristics, flaB2 gene sequences, enzyme profiles and cell morphology confirmed that they formed a coherent taxonomic group displaying marked genotypic and phenotypic differences with respect to recognized treponeme species. The four novel isolates displayed a novel 3 : 6 : 3 flagellar pattern rather than the 2 : 4 : 2 pattern shown by their closest relatives and exhibited esterase C4, esterase lipase C8, trypsin and chymotrypsin enzyme activities. Therefore these four new isolates represent a novel species of the genus Treponema, for which the name Treponema pedis sp. nov. is proposed. The type strain is T3552B(T) (=DSM 18691(T)=NCTC 13403(T)).

Authors+Show Affiliations

Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 3BX, UK. evansnj@liverpool.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19406779

Citation

Evans, Nicholas J., et al. "Treponema Pedis Sp. Nov., a Spirochaete Isolated From Bovine Digital Dermatitis Lesions." International Journal of Systematic and Evolutionary Microbiology, vol. 59, no. Pt 5, 2009, pp. 987-91.
Evans NJ, Brown JM, Demirkan I, et al. Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions. Int J Syst Evol Microbiol. 2009;59(Pt 5):987-91.
Evans, N. J., Brown, J. M., Demirkan, I., Murray, R. D., Birtles, R. J., Hart, C. A., & Carter, S. D. (2009). Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions. International Journal of Systematic and Evolutionary Microbiology, 59(Pt 5), 987-91. https://doi.org/10.1099/ijs.0.002287-0
Evans NJ, et al. Treponema Pedis Sp. Nov., a Spirochaete Isolated From Bovine Digital Dermatitis Lesions. Int J Syst Evol Microbiol. 2009;59(Pt 5):987-91. PubMed PMID: 19406779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions. AU - Evans,Nicholas J, AU - Brown,Jennifer M, AU - Demirkan,Ibrahim, AU - Murray,Richard D, AU - Birtles,Richard J, AU - Hart,C Anthony, AU - Carter,Stuart D, PY - 2009/5/2/entrez PY - 2009/5/2/pubmed PY - 2009/7/8/medline SP - 987 EP - 91 JF - International journal of systematic and evolutionary microbiology JO - Int. J. Syst. Evol. Microbiol. VL - 59 IS - Pt 5 N2 - Bovine digital dermatitis (BDD) is a debilitating infection that is being increasingly recognized in livestock worldwide. Several treponeme phylotypes have been identified in BDD lesions, although only a single BDD-associated treponeme taxon (Treponema brennaborense) has been proposed thus far. In a previous study, we observed that four BDD-associated spirochaete isolates formed a distinct phylogenetic cluster on the basis of 16S rRNA gene sequence analysis and shared less than 97 % sequence similarity with recognized treponeme species. Further characterization of these isolates on the basis of growth characteristics, flaB2 gene sequences, enzyme profiles and cell morphology confirmed that they formed a coherent taxonomic group displaying marked genotypic and phenotypic differences with respect to recognized treponeme species. The four novel isolates displayed a novel 3 : 6 : 3 flagellar pattern rather than the 2 : 4 : 2 pattern shown by their closest relatives and exhibited esterase C4, esterase lipase C8, trypsin and chymotrypsin enzyme activities. Therefore these four new isolates represent a novel species of the genus Treponema, for which the name Treponema pedis sp. nov. is proposed. The type strain is T3552B(T) (=DSM 18691(T)=NCTC 13403(T)). SN - 1466-5026 UR - https://www.unboundmedicine.com/medline/citation/19406779/Treponema_pedis_sp__nov__a_spirochaete_isolated_from_bovine_digital_dermatitis_lesions_ L2 - http://ijs.microbiologyresearch.org/pubmed/content/journal/ijsem/10.1099/ijs.0.002287-0 DB - PRIME DP - Unbound Medicine ER -