Tags

Type your tag names separated by a space and hit enter

Purification, crystallization and preliminary X-ray analysis of isocitrate dehydrogenase kinase/phosphatase from Escherichia coli.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 01; 65(Pt 5):536-9.AC

Abstract

The Escherichia coli aceK gene encodes isocitrate dehydrogenase kinase/phosphatase (EC 2.7.11.5), a bifunctional protein that phosphorylates and dephosphorylates isocitrate dehydrogenase (IDH), resulting in its inactivation and activation, respectively. This reversible (de)phosphorylation directs isocitrate, an intermediate of the citric acid cycle, to either go through the full cycle or to enter the glyoxylate bypass. In the present study, the AceK protein from E. coli has been purified and crystallized. Three crystal forms were obtained from very similar crystallization conditions. The crystals belong to space groups P4(1)2(1)2, P3(2)21 and P2(1)2(1)2(1) and diffracted X-rays to resolutions of 2.9, 3.0 and 2.7 A, respectively.

Authors+Show Affiliations

Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19407397

Citation

Zheng, Jimin, et al. "Purification, Crystallization and Preliminary X-ray Analysis of Isocitrate Dehydrogenase Kinase/phosphatase From Escherichia Coli." Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 65, no. Pt 5, 2009, pp. 536-9.
Zheng J, Lee DC, Jia Z. Purification, crystallization and preliminary X-ray analysis of isocitrate dehydrogenase kinase/phosphatase from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65(Pt 5):536-9.
Zheng, J., Lee, D. C., & Jia, Z. (2009). Purification, crystallization and preliminary X-ray analysis of isocitrate dehydrogenase kinase/phosphatase from Escherichia coli. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 65(Pt 5), 536-9. https://doi.org/10.1107/S1744309109014729
Zheng J, Lee DC, Jia Z. Purification, Crystallization and Preliminary X-ray Analysis of Isocitrate Dehydrogenase Kinase/phosphatase From Escherichia Coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt 5):536-9. PubMed PMID: 19407397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, crystallization and preliminary X-ray analysis of isocitrate dehydrogenase kinase/phosphatase from Escherichia coli. AU - Zheng,Jimin, AU - Lee,Daniel C, AU - Jia,Zongchao, Y1 - 2009/04/28/ PY - 2009/01/21/received PY - 2009/04/20/accepted PY - 2009/5/2/entrez PY - 2009/5/2/pubmed PY - 2009/6/20/medline SP - 536 EP - 9 JF - Acta crystallographica. Section F, Structural biology and crystallization communications JO - Acta Crystallogr Sect F Struct Biol Cryst Commun VL - 65 IS - Pt 5 N2 - The Escherichia coli aceK gene encodes isocitrate dehydrogenase kinase/phosphatase (EC 2.7.11.5), a bifunctional protein that phosphorylates and dephosphorylates isocitrate dehydrogenase (IDH), resulting in its inactivation and activation, respectively. This reversible (de)phosphorylation directs isocitrate, an intermediate of the citric acid cycle, to either go through the full cycle or to enter the glyoxylate bypass. In the present study, the AceK protein from E. coli has been purified and crystallized. Three crystal forms were obtained from very similar crystallization conditions. The crystals belong to space groups P4(1)2(1)2, P3(2)21 and P2(1)2(1)2(1) and diffracted X-rays to resolutions of 2.9, 3.0 and 2.7 A, respectively. SN - 1744-3091 UR - https://www.unboundmedicine.com/medline/citation/19407397/Purification_crystallization_and_preliminary_X_ray_analysis_of_isocitrate_dehydrogenase_kinase/phosphatase_from_Escherichia_coli_ L2 - http://scripts.iucr.org/cgi-bin/paper?S1744309109014729 DB - PRIME DP - Unbound Medicine ER -